Recombinant Human Siglec-2/CD22 Fc Chimera Protein, CF

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1968-SL-050 In Stock50 ug / $400

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Recombinant Human Siglec-2/CD22 Fc Chimera Protein, CF Summary

Why choose R&D Systems Siglec-2 Protein?

  • Guaranteed Bioactivity and High Purity: Bioactivity tested by functional ELISA and purity determined by SDS-PAGE to be greater than 95%.
  • Lot-to-Lot Consistency: Stringent QC testing performed on each lot to ensure consistent activity and purity.
  • Bulk Quantities Available: Bulk up and save with large mass quantities to meet your research needs. Supply agreements available, partner with us. Please contact us.
  • Most Respected, Most Cited Brand in Proteins: With over 35 years of providing the best recombinant proteins to the scientific community, R&D Systems continues to lead the industry in quality, activity, and purity.

NEW! Fluorescent labeled Siglec-2 designed for validating expression of a Siglec-2 chimeric antigen receptor (CAR). Avi-tag Siglec-2 also available. Siglec-2 Proteins

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of human red blood cells. Kelm, S. et al. (1994) Current Biology 4:965. The ED50 for this effect is 0.03-0.15 µg/mL.
Source
Mouse myeloma cell line, NS0-derived human Siglec-2/CD22 protein
Human Siglec-2
(Asp20-Arg687)
Accession # CAA42006
DIEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Asp20
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
101.9 kDa (monomer)
SDS-PAGE
122-127 kDa, reducing conditions

Product Datasheets

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1968-SL

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1968-SL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Background: Siglec-2/CD22

Siglecs (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins belonging to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains (1, 2). Eleven human Siglecs have been cloned and characterized. They are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3, Myelin-Associated Glycoprotein (MAG/Siglec-4a) and the identified Siglecs 5 to 11 (1 ‑ 3). To date, no Siglec has been shown to recognize any cell surface ligand other than sialic acid, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs.

Human Siglec-2, also known as B-cell antigen CD22 or B-lymphocyte cell adhesion molecule (BL-CAM), is a B-cell restricted glycoprotein that is expressed in the cytoplasm of progenitor B and pre-B cells and on the surface of mature B cells. Two distinct human Siglec-2/CD22 cDNAs that arise from differential RNA processing of the same gene have been isolated. The predominant Siglec-2/CD22 beta encodes an 847 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-terminal Ig-like V-type domain, six Ig-like C2-type domains, a transmembrane region and a cytoplasmic tail with 4 immunoreceptor tyrosine-based inhibition motifs (ITIMs) (4). The variant Siglec-2/CD22 alpha encodes a 647 aa polypeptide missing two Ig-like C2-type domains and has a truncated (23 aa) cytoplasmic tail (5). Siglec-2/CD22 is an adhesion molecule that preferentially binds alpha 2,6- linked sialic acid on the same (cis) or adjacent (trans) cells. Interaction of CD22 with trans ligands on opposing cells was found to be favored over the binding of ligands in cis (9). Besides its role as an adhesion molecule, Siglec-2/CD22 is a coreceptor that physically interacts with B-cell receptor (BCR) and is rapidly phosphorylated upon BCR ligation. It negatively regulates BCR signals by recruiting tyrosine phosphatase SHP-1 to its ITIMs. Phosphorylated Siglec-2/CD22 can also interact with other intracellular effector proteins such as Syk, PLC gamma, PI3 kinase and Grb-2, suggesting it may play a role in positive signaling (2, 7, 8).

References
  1. Crocker, P.R. and A. Varki (2001) Trends Immunol. 22:337.
  2. Crocker, P.R. and A. Varki (2001) Immunology 103:137.
  3. Angata, T. et al. (2002) J. Biol. Chem. 277:24466.
  4. Wilson, G.L et al. (1991) J. Exp. Med. 173:137.
  5. Stamenkovic, I. and B. Seed (1990) Nature 345:74.
  6. Kelm, S. et al. (1994) Current Bio. 4:965.
  7. Ravetch, J.V. and L.L. Lanier (2000) Science 290:84.
  8. Wienands, Y.J. et al. (1999) J. Biol. Chem. 274:18769.
  9. Collins, B.E. et al. (2004) Proc. Natl. Acad. Sci. 101:6104.
Long Name
Sialic Acid Binding Ig-like Lectin 2
Entrez Gene IDs
933 (Human); 12483 (Mouse); 308501 (Rat); 102130968 (Cynomolgus Monkey)
Alternate Names
B-cell receptor CD22; BL-CAM; B-lymphocyte cell adhesion molecule; CD22 antigenMGC130020; CD22 molecule; CD22; sialic acid binding Ig-like lectin 2; Sialic acid-binding Ig-like lectin 2; Siglec2; Siglec-2; SIGLEC2FLJ22814; T-cell surface antigen Leu-14

Citations for Recombinant Human Siglec-2/CD22 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Efficient chimeric antigen receptor (CAR) targeting of a central epitope of CD22
    Authors: Casey, NP;Klee, CH;Fåne, A;Caulier, B;Graczyk-Jarzynka, A;Krawczyk, M;Fidyt, K;Josefsson, SE;Köksal, H;Dillard, P;Patkowska, E;Firczuk, M;Smeland, EB;Winiarska, M;Myklebust, JH;Inderberg, EM;Wälchli, S;
    The Journal of biological chemistry
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Massively parallel knock-in engineering of human T cells
    Authors: X Dai, JJ Park, Y Du, Z Na, SZ Lam, RD Chow, PA Renauer, J Gu, S Xin, Z Chu, C Liao, P Clark, H Zhao, S Slavoff, S Chen
    Nature Biotechnology, 2023-01-26;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Antigen-independent activation enhances the efficacy of 4-1BB-costimulated CD22 CAR T cells
    Authors: N Singh, NV Frey, B Engels, DM Barrett, O Shestova, P Ravikumar, KD Cummins, YG Lee, R Pajarillo, I Chun, A Shyu, SL Highfill, A Price, L Zhao, L Peng, B Granda, M Ramones, XM Lu, DA Christian, J Perazzelli, SF Lacey, NH Roy, JK Burkhardt, F Colomb, M Damra, M Abdel-Mohs, T Liu, D Liu, DM Standley, RM Young, JL Brogdon, SA Grupp, CH June, SL Maude, S Gill, M Ruella
    Nature Medicine, 2021-04-22;0(0):.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  4. Lectin nanoparticle assays for detecting breast cancer-associated glycovariants of cancer antigen 15-3 (CA15-3) in human plasma
    Authors: J Terävä, L Tiainen, U Lamminmäki, PL Kellokumpu, K Pettersson, K Gidwani
    PLoS ONE, 2019-07-25;14(7):e0219480.
    Species: Human
    Sample Types: Natural Protein
    Applications: Bioassay
  5. CD22-targeted CAR T cells induce remission in B-ALL that is naive or resistant to CD19-targeted CAR immunotherapy
    Authors: TJ Fry, NN Shah, RJ Orentas, M Stetler-St, CM Yuan, S Ramakrishn, P Wolters, S Martin, C Delbrook, B Yates, H Shalabi, TJ Fountaine, JF Shern, RG Majzner, DF Stroncek, M Sabatino, Y Feng, DS Dimitrov, L Zhang, S Nguyen, H Qin, B Dropulic, DW Lee, CL Mackall
    Nat. Med., 2017-11-20;24(1):20-28.
    Species: Human
    Sample Types: Whole Cells
    Applications: CAR-T (Flow Cytometry)
  6. Broad and direct interaction between TLR and Siglec families of pattern recognition receptors and its regulation by Neu1.
    Authors: Chen, Guo-Yun, Brown, Nicholas, Wu, Wei, Khedri, Zahra, Yu, Hai, Chen, Xi, van de Vlekkert, Diantha, D'Azzo, Alessand, Zheng, Pan, Liu, Yang
    Elife, 2014-09-03;3(0):e04066.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  7. Anti-CD22-chimeric antigen receptors targeting B-cell precursor acute lymphoblastic leukemia.
    Authors: Haso W, Lee D, Shah N, Stetler-Stevenson M, Yuan C, Pastan I, Dimitrov D, Morgan R, Fitzgerald D, Barrett D, Wayne A, Mackall C, Orentas R
    Blood, 2012-12-14;121(7):1165-74.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Generation, affinity maturation, and characterization of a human anti-human NKG2D monoclonal antibody with dual antagonistic and agonistic activity.
    Authors: Kwong KY, Baskar S, Zhang H, Mackall CL, Rader C
    J. Mol. Biol., 2008-09-16;384(5):1143-56.
    Species: Human
    Sample Types: Antibody
    Applications: Bioassay

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Reviews for Recombinant Human Siglec-2/CD22 Fc Chimera Protein, CF

Average Rating: 4.7 (Based on 3 Reviews)

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Recombinant Human Siglec-2/CD22 Fc Chimera Protein, CF
By Anonymous on 11/21/2019
Application: Characterization prior to use in assay.
Reason for Rating: SEC percent monomer about 83%

Recombinant Human Siglec-2/CD22 Fc Chimera Protein, CF
By Jenna Nguyen on 11/17/2018
Application: In vivo study

Recombinant Human Siglec-2/CD22 Fc Chimera Protein, CF
By Jenna Nguyen on 07/11/2018
Application: Apoptosis assay