Recombinant Mouse Siglec-2/CD22 Fc Chimera Protein, CF
Recombinant Mouse Siglec-2/CD22 Fc Chimera Protein, CF Summary
Product Specifications
| Mouse Siglec-2 (Ser22 - Arg702) Accession # P35329 |
IEGRMD | Human IgG1 (Pro100 - Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
2296-SL
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Siglec-2/CD22
Siglecs (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins belonging to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains (1, 2). Eleven human Siglecs have been cloned and characterized. Among these are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2 and CD33/Siglec-3. To date, no Siglec has been shown to recognize any cell surface ligand other than sialic acid, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs. The cDNA of mouse Siglec-2 (also known as B-cell antigen CD22), encodes an 862 amino acid (aa) protein that contains a 21 aa signal peptide, a 681 aa extracellular region, a 19 aa transmembrane region and a 141 aa cytoplasmic tail (3, 4). The extracellular region contains one N-terminal V-type Ig-like domain followed by six Ig-like C2-type domains. The cytoplasmic domain has 3 immunoreceptor tyrosine-based inhibition motifs (ITIMs). Two splice forms exist, both showing deletions in the V-type Ig domain of 30 aa and 60 aa each. There are also two alleles in mouse that account for a difference of 10 aa in the extracellular region. The extracellular region of mouse Siglec-2 is 60% aa identity to human extracellular Siglec-2. Expression of mouse Siglec-2/CD22 generates a 140 kDa integral membrane glycoprotein that is limited to the B cell compartment of lymphoid tissues. Its expression is upregulated by LPS activtion (5, 6). Siglec-2/CD22 is an adhesion molecule that preferentially binds alpha 2,6- linked sialic acid on the same (cis) or adjacent (trans) cells. Interaction of CD22 with trans ligands on opposing cells was found to be favored over the binding of ligands in cis (7).
- Nitschke, L. et al. (2001) Scand. J. Immunol. 53:227.
- Crocker, P.R. and A. Varki (2001) Immunology 103:137.
- Law, C-L. et al. (1993) J. Immunol. 151:175.
- Wienands, Y.J. et al. (1999) J. Biol. Chem. 274:18769.
- Wilson, G.L et al. (1991) J. Exp. Med. 173:137.
- Torres, P.M. (1992) J. Immunol. 194:2641.
- Collins, B.E. et al. (2004) Proc. Natl. Acad. Sci. 101:6104.
Citations for Recombinant Mouse Siglec-2/CD22 Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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CD22 blockade restores homeostatic microglial phagocytosis in ageing brains
Authors: JV Pluvinage, MS Haney, BAH Smith, J Sun, T Iram, L Bonanno, L Li, DP Lee, DW Morgens, AC Yang, SR Shuken, D Gate, M Scott, P Khatri, J Luo, CR Bertozzi, MC Bassik, T Wyss-Coray
Nature, 2019-04-03;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Broad and direct interaction between TLR and Siglec families of pattern recognition receptors and its regulation by Neu1.
Authors: Chen, Guo-Yun, Brown, Nicholas, Wu, Wei, Khedri, Zahra, Yu, Hai, Chen, Xi, van de Vlekkert, Diantha, D'Azzo, Alessand, Zheng, Pan, Liu, Yang
Elife, 2014-09-03;3(0):e04066.
Species: Mouse
Sample Types: Cell Lysates
Applications: Bioassay -
Transcriptional programs of lymphoid tissue capillary and high endothelium reveal control mechanisms for lymphocyte homing.
Authors: Lee, Mike, Kiefel, Helena, LaJevic, Melissa, Macauley, Matthew, Kawashima, Hiroto, O'Hara, Edward, Pan, Junliang, Paulson, James C, Butcher, Eugene C
Nat Immunol, 2014-08-31;15(10):982-95.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
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