Recombinant Mouse CRISP-3 Protein, CF

Catalog #: 7056-CR Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
7056-CR-050

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Recombinant Mouse CRISP-3 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit proliferation/survival of the 3A‑sub E human placenta cell line.

The ED₅₀ for this effect is 1.3-5.2 μg/mL.

Source

Mouse myeloma cell line, NS0-derived mouse CRISP-3 protein
Gln20-Cys241, with a C-terminal 6-His tag

Accession #

Q03402

N-terminal Sequence
Analysis

No results obtained: Gln20 predicted (N-sequencing might be blocked)

Predicted Molecular Mass

26 kDa

SDS-PAGE

30-40 kDa, reducing conditions

Product Datasheets

7056-CR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7056-CR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: CRISP-3

CRISP-3 is one of three CRISPs (cysteine-rich secretory proteins) found in mammalian exocrine secretions and granulocytes that may play a role in innate immunity (1 - 3). CRISPs and several snake, insect, and lizard venom proteins are characterized by 16 invariant cysteine residues (4). Structurally, they consist of an N-terminal SCP domain, a hinge region, and a cysteine-rich domain (5). Human CRISP-3 is produced by salivary, pancreas, prostate, and lacrimal glands, as well as spermatozoa and mature spermatids (2, 6, 7). In mouse, CRISP-3 has not been detected in the male genital tract, although its salivary gland expression is androgen-dependent (8, 9). CRISP-3 is up‑regulated in human epithelial prostate cancer and chronic pancreatitis (10, 11). It is present as 30 kDa and 28 kDa species, corresponding to glycosylated and nonglycosylated forms (1, 3, 7, 10, 12). In human serum and seminal fluid, CRISP-3 forms high affinity noncovalent complexes with the more abundant alpha 1B-glycoprotein and beta ‑microseminoprotein/PSP94, respectively (12, 13). Binding is mediated by the SCP domain of CRISP-3 and is independent of glycosylation (12). CRISP-3 is also expressed in pre-B cells but not in T cells or monocytes and is released from human neutrophil and eosinophil granules following cell stimulation (1, 14, 15). Mature mouse CRISP-3 shares 48% and 64% amino acid (aa) sequence identity with human and equine CRISP-3, respectively. It shares 73% and 47% aa sequence identity with mouse CRISP-1 and -2, respectively.

References

Kjeldsen, L. et al. (1996) FEBS Lett. 380:246.
Kratzschmar, J. et al. (1996) Eur. J. Biochem. 236:827.
Udby, L. et al. (2002) J. Immunol. Meth. 263:43.
Yamazaki, Y. and Morita, T. (2004) Toxicon 44:227.
Guo, M. et al. (2005) J. Biol. Chem. 280:12405.
Haendler, B. et al. (1999) J. Cell. Physiol. 178:371.
Udby, L. et al. (2005) J. Androl. 26:333.
Haendler, B. et al. (1993) Endocrinology 133:192.
Haendler, B. et al. (1997) Eur. J. Biochem. 250:440.
Bjartell, A. et al. (2006) Prostate 66:591.
Liao, Q. et al. (2003) Histol. Histopathol. 18:425.
Udby, L. et al. (2005) Biochem. Biophys. Res. Commun. 333:555.
Udby, L. et al. (2004) Biochemistry 43:12877.
Pfisterer, P. et al. (1996) Mol. Cell. Biol. 16:6160.
Udby, L. et al. (2002) J. Leukoc. Biol. 72:462.

Long Name

Cysteine-rich Secretory Protein 3

Entrez Gene IDs

10321 (Human); 11572 (Mouse)

Alternate Names

Aeg2; CRISP3; CRISP-3; CRISP-3MGC126588; CRS3; cysteine-rich secretory protein 3; cysteine-rich secretory protein-3; dJ442L6.3; SGP28; SGP28Aeg2; specific granule protein (28 kDa); Specific granule protein of 28 kDa