Recombinant Mouse E-Cadherin Fc Chimera Protein, CF

Name: Recombinant Mouse E-Cadherin Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 748-EC

Catalog #: 748-EC
8 Citations Datasheet / COA / SDS

Newer Version Available: 8875-EC

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Discontinued Product

748-EC has been discontinued and is replaced by 8875-EC.

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Citations (8)

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse E-Cadherin Fc Chimera Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by the ability of the immobilized protein to support the adhesion of the MCF‑7 human breast cancer cells. When 5 x 10⁴ cells/well are added to Recombinant Mouse E-Cadherin Fc Chimera coated plates (1.5 µg/mL with 100 µL/well), >50% cells will adhere after 90 minutes at 37 °C.
Optimal dilutions should be determined by each laboratory for each application.

Source

Mouse myeloma cell line, NS0-derived mouse E-Cadherin protein

Mouse E-Cadherin (Asp157-Val709) Accession # P09803	IEGRMD	Human IgG₁ (Pro100-Lys330)	6-His tag
N-terminus			C-terminus

Accession #

P09803

N-terminal Sequence
Analysis

Asp157

Predicted Molecular Mass

88.2 kDa (monomer)

SDS-PAGE

120 kDa, reducing conditions

Product Datasheets

748-EC

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

748-EC

Formulation	Lyophilized from a 0.2 μm filtered solution in Tris-Citrate, NaCl and CaCl₂.
Reconstitution	Reconstitute at 100 μg/mL in sterile DPBS with Ca²⁺ and Mg²⁺.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: E-Cadherin

E-Cadherin/Cadherin-1, also known as Uvomorulin in the mouse and rat, is a 120 kDa member of the Cadherin family of cell surface glycoproteins that mediate cell adhesion (1). Mouse E-Cadherin shares 92% and 81% amino acid sequence identity with the rat and human proteins, respectively (2). It is a single-pass transmembrane protein that mediates calcium-dependent epithelial cell adhesion. E-Cadherin has five extracellular EC domains that form homophilic cis-clusters between adjacent epithelial cells and trans-clusters within the same cell. E-Cadherin clusters are critical components of adherens junctions between epithelial cells and act in the formation and maintenance of the epithelial cell barrier (3, 4). The intracellular domain of E-Cadherin binds to the Catenin cytoskeletal complex, which includes p120 Catenin, beta-Catenin, alpha-Catenin, and Vinculin. E-Cadherin expression is critical for epithelial tissue homeostasis. Decreased E-Cadherin is associated with physiological and pathological epithelial-to-mesenchymal transition and cell migration, and E-Cadherin loss contributes to cancer metastasis (5). The extracellular E-Cadherin N-terminal domain can be cleaved by several proteases and is released as a soluble factor that enhances cancer cell motility and EGF R‑dependent survival and proliferation (6).

References

Gumbiner, B.M. (2005) Nat. Rev. Mol. Cell Biol. 6:622.
Nagafuchi, A. et al. (1987) Nature 329:341.
Guillot, C. and T. Lecuit (2013) Science 340:1185.
Tian, X. et al. (2011) J. Biomed. Biotechnol. 2011:567305.
Stemmler, M.P. (2008) Mol. Biosyst. 4:835.
David, J.M. and A.K. Rajasekaran (2012) Cancer Res. 72:2917.

Entrez Gene IDs

999 (Human); 12550 (Mouse); 83502 (Rat)

Alternate Names

Arc-1; CAD1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1, E-cadherin (epithelial); Cadherin-1; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324 antigen; CD324; CDH1; CDHE; cell-CAM 120/80; Cell-CAM120/80; ECAD; ECadherin; E-Cadherin; Epithelial cadherin; LCAM; L-CAM; UVOE-Cadherin; Uvomorulin

Related Research Areas

Citations for Recombinant Mouse E-Cadherin Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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DamC reveals principles of chromatin folding in vivo without crosslinking and ligation
Authors: J Redolfi, Y Zhan, C Valdes-Que, M Kryzhanovs, I Guerreiro, V Iesmantavi, T Pollex, RS Grand, E Mulugeta, J Kind, G Tiana, SA Smallwood, W de Laat, L Giorgetti
Nat. Struct. Mol. Biol., 2019-05-27;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture
Local Integrin Activation in Pancreatic ? Cells Targets Insulin Secretion to the Vasculature
Authors: WJ Gan, OH Do, L Cottle, W Ma, E Kosobrodov, J Cooper-Whi, M Bilek, P Thorn
Cell Rep, 2018-09-11;24(11):2819-2826.e3.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
Network plasticity of pluripotency transcription factors in embryonic stem cells.
Authors: Filipczyk A, Marr C, Hastreiter S, Feigelman J, Schwarzfischer M, Hoppe P, Loeffler D, Kokkaliaris K, Endele M, Schauberger B, Hilsenbeck O, Skylaki S, Hasenauer J, Anastassiadis K, Theis F, Schroeder T
Nat Cell Biol, 2015-09-21;17(10):1235-46.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
Chondroitin sulfate is indispensable for pluripotency and differentiation of mouse embryonic stem cells.
Authors: Izumikawa, Tomomi, Sato, Ban, Kitagawa, Hiroshi
Sci Rep, 2014-01-15;4(0):3701.
Species: Mouse
Sample Types: Protein
Applications: Binding Assay
Soluble E-cadherin: a critical oncogene modulating receptor tyrosine kinases, MAPK and PI3K/Akt/mTOR signaling.
Authors: Brouxhon, S M, Kyrkanides, S, Teng, X, Athar, M, Ghazizadeh, S, Simon, M, O'Banion, M K, Ma, L
Oncogene, 2013-01-14;33(2):225-35.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
The ectodomain shedding of E-cadherin by ADAM15 supports ErbB receptor activation.
Authors: Najy AJ, Day KC, Day ML
J. Biol. Chem., 2008-04-22;283(26):18393-401.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
Early loss of E-cadherin from cell-cell contacts is involved in the onset of Anoikis in enterocytes.
Authors: Fouquet S, Lugo-Martinez VH, Faussat AM, Renaud F, Cardot P, Chambaz J, Pincon-Raymond M, Thenet S
J. Biol. Chem., 2004-08-02;279(41):43061-9.
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay
The alpha 1 beta 1 and alpha E beta 7 integrins define a subset of dendritic cells in peripheral lymph nodes with unique adhesive and antigen uptake properties.
Authors: Pribila JT, Itano AA, Mueller KL, Shimizu Y
J. Immunol., 2004-01-01;172(1):282-91.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay