Infection of mosquito-borne West Nile Virus can cause severe neurological disease and can be epidemic. Two non-structural proteins, NS3 and NS2b, play an essential role in viral replication and are therefore a potential target for treatment and prevention of West Nile Virus disease. NS3 consists of a trypsin-like serine protease with a catalytic triad (His51, Asp75, Ser135) and a putative helicase. Requiring NS2b as the cofactor, NS3 protease processes viral polyprotein precursor (1, 2). The purified recombinant protein used as an immunogen consists of three forms: the full-length fusion protein, the N-terminal NS2b, and the C-terminal NS3 with a G4SG4 linker. NS3 protease has a relatively narrow substrate specificity that prefers Arg in P1 and Lys in P2.
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
sequences: rwnvNS2b 1-48, rwnvNS3 1-184
Specificity
Clonality
Host
Isotype
Scientific Data Images for Viral wnvNS3 Protease Antibody
Viral wnvNS3 Protease ELISA Standard Curve.
Recombinant Viral wnvNS3 Protease protein was serially diluted 2-fold and captured by Mouse Anti-Viral wnvNS3 Protease Monoclonal Antibody (Catalog # MAB29074) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Viral wnvNS3 Protease Monoclonal Antibody (Catalog # MAB29073) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Applications for Viral wnvNS3 Protease Antibody
ELISA
This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Viral wnvNS3 Protease Monoclonal Antibody (Catalog # MAB29074).
This product is intended for assay development on various assay platforms requiring antibody pairs.
Formulation, Preparation, and Storage
Purification
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: wnvNS3 Protease
References
- Nall, T.A. et al. (2004) J. Biol. Chem. 279:48535.
- Chappell, K.J. et al. (2005) J. Biol. Chem. 274:2896.
Long Name
Entrez Gene IDs
Gene Symbol
Additional wnvNS3 Protease Products
Product Documents for Viral wnvNS3 Protease Antibody
Product Specific Notices for Viral wnvNS3 Protease Antibody
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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