Viral wnvNS3 Protease Antibody

Catalog #: MAB29073 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB29073-100
MAB29073-SP

Viral wnvNS3 Protease ELISA Standard Curve.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Viral wnvNS3 Protease Antibody Summary

Species Reactivity

Viral

Specificity

Detects viral wnvNS3 Protease in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 991926

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived Viral wnvNS3 Protease
sequences: rwnvNS2b 1-48, rwnvNS3 1-184

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Viral wnvNS3 Protease Monoclonal Antibody (Catalog # MAB29074).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Viral wnvNS3 Protease ELISA Standard Curve. Recombinant Viral wnvNS3 Protease protein was serially diluted 2-fold and captured by Mouse Anti-Viral wnvNS3 Protease Monoclonal Antibody (Catalog # MAB29074) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Viral wnvNS3 Protease Monoclonal Antibody (Catalog # MAB29073) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: wnvNS3 Protease

Infection of mosquito-borne West Nile Virus can cause severe neurological disease and can be epidemic. Two non-structural proteins, NS3 and NS2b, play an essential role in viral replication and are therefore a potential target for treatment and prevention of West Nile Virus disease. NS3 consists of a trypsin-like serine protease with a catalytic triad (His51, Asp75, Ser135) and a putative helicase. Requiring NS2b as the cofactor, NS3 protease processes viral polyprotein precursor (1, 2). The purified recombinant protein used as an immunogen consists of three forms: the full-length fusion protein, the N-terminal NS2b, and the C-terminal NS3 with a G₄SG₄ linker. NS3 protease has a relatively narrow substrate specificity that prefers Arg in P1 and Lys in P2.

References