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Goat IgG PE-conjugated Antibody

Catalog #: IC108P
24 Citations 5 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
IC108P
Detection of Goat IgG Isotype Control by Flow Cytometry
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Product Details
Citations (24)
FAQs
Supplemental Products
Reviews (5)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Goat IgG PE-conjugated Antibody Summary

Specificity
Serum was obtained from naive (non-immunized) goats.
Source
Polyclonal Goat IgG
Purification
Protein A or G purified
Immunogen
NA
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Control

Negative control for use in conjunction with R&D Systems antibodies for flow cytometry applications. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems specific polyclonal antibodies. The recommended concentration is 10 μL/106 cells.

 

This reagent has not been tested for immunohistochemistry.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Control Detection of Goat IgG Isotype Control by Flow Cytometry View Larger

Detection of Goat IgG Control by Flow Cytometry bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Mouse VEGF R3/Flt-4 PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB743P, filled histogram) or Goat IgG PE-conjugated Isotype Control Antibody (Catalog # IC108P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; Immunoglobulin G; ImmunoglobulinG

Product Datasheets

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Citations for Goat IgG PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

24 Citations: Showing 1 - 10
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  1. GATA6 regulates WNT and BMP programs to pattern precardiac mesoderm during the earliest stages of human cardiogenesis
    Authors: Bisson, JA;Gordillo, M;Kumar, R;de Silva, N;Yang, E;Banks, KM;Shi, ZD;Lee, K;Yang, D;Chung, WK;Huangfu, D;Evans, T;
    bioRxiv : the preprint server for biology
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  2. Upper Airway Alarmin Cytokine Expression in Asthma of Different Severities
    Authors: Marriott, H;Duchesne, M;Moitra, S;Okoye, I;Gerla, L;Mayers, I;Moolji, J;Adatia, A;Lacy, P;
    Journal of clinical medicine
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  3. Inhibition of interleukin-1beta reduces myelofibrosis and osteosclerosis in mice with JAK2-V617F driven myeloproliferative neoplasm
    Authors: S Rai, E Grockowiak, N Hansen, D Luque Paz, CB Stoll, H Hao-Shen, G Mild-Schne, S Dirnhofer, CJ Farady, S Méndez-Fer, RC Skoda
    Nature Communications, 2022-09-13;13(1):5346.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. TCF-1 controls Treg cell functions that regulate inflammation, CD8+ T cell cytotoxicity and severity of colon cancer
    Authors: A Osman, B Yan, Y Li, KD Pavelko, J Quandt, A Saadalla, MP Singh, M Kazemian, F Gounari, K Khazaie
    Nature Immunology, 2021-08-12;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. A variable-gain stochastic pooling motif mediates information transfer from receptor assemblies into NF-kappa B
    Authors: J. Agustin Cruz, Chaitanya S. Mokashi, Gabriel J. Kowalczyk, Yue Guo, Qiuhong Zhang, Sanjana Gupta et al.
    Science Advances
  6. JAK1 Inhibition Blocks Lethal Immune Hypersensitivity in a Mouse Model of Down Syndrome
    Authors: KD Tuttle, KA Waugh, P Araya, R Minter, DJ Orlicky, M Ludwig, Z Andrysik, MA Burchill, BAJ Tamburini, C Sempeck, K Smith, R Granrath, D Tracy, J Baxter, JM Espinosa, KD Sullivan
    Cell Rep, 2020-11-17;33(7):108407.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  7. Radiation Triggers a Dynamic Sequence of Transient Microglial Alterations in Juvenile Brain
    Authors: AM Osman, Y Sun, TC Burns, L He, N Kee, N Oliva-Vila, A Alevyzaki, K Zhou, L Louhivuori, P Uhlén, E Hedlund, C Betsholtz, VM Lauschke, J Kele, K Blomgren
    Cell Rep, 2020-06-02;31(9):107699.
    Species: Mouse
    Sample Types:
    Applications: IHC
  8. Different immunological effects of the molecular targeted agents sunitinib, everolimus and temsirolimus in patients with renal cell carcinoma
    Authors: Yukari Kobayashi, Daisuke Yamada, Taketo Kawai, Yusuke Sato, Taro Teshima, Yuta Yamada et al.
    International Journal of Oncology
  9. Damage- and pathogen-associated molecular patterns play differential roles in late mortality after critical illness
    Authors: John Eppensteiner, Jean Kwun, Uwe Scheuermann, Andrew Barbas, Alexander T. Limkakeng, Maggie Kuchibhatla et al.
    JCI Insight
  10. Mycobacterium tuberculosis Inhibits Autocrine Type I IFN Signaling to Increase Intracellular Survival
    Authors: DA Banks, SE Ahlbrand, VK Hughitt, S Shah, KD Mayer-Barb, SN Vogel, NM El-Sayed, V Briken
    J. Immunol., 2019-03-04;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Regulation of radiosensitivity by 4-methylumbelliferone via the suppression of interleukin-1 in fibrosarcoma cells
    Authors: R Saga, K Hasegawa, K Murata, M Chiba, T Nakamura, K Okumura, E Tsuruga, Y Hosokawa
    Oncol Lett, 2019-01-29;17(3):3555-3561.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Cancer-associated mutations in the canonical cleavage site do not influence CD99 shedding by the metalloprotease meprin beta but alter cell migration in vitro
    Authors: Tillmann Bedau, Neele Schumacher, Florian Peters, Johannes Prox, Philipp Arnold, Tomas Koudelka et al.
    Oncotarget
  13. Elevated expression of Tie1 is accompanied by acquisition of cancer stemness properties in colorectal cancer
    Authors: M Torigata, D Yamakawa, N Takakura
    Cancer Med, 2017-05-02;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Structural characterisation of high affinity Siglec-2 (CD22) ligands in complex with whole Burkitt's lymphoma (BL) Daudi cells by NMR spectroscopy
    Sci Rep, 2016-11-03;6(0):36012.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  15. Capsaicin induces immunogenic cell death in human osteosarcoma cells
    Authors: Tao Jin, Hongyan Wu, Yanlin Wang, Hao Peng
    Experimental and Therapeutic Medicine
  16. Transgenic Overexpression of Tissue‐Nonspecific Alkaline Phosphatase (TNAP) in Vascular Endothelium Results in Generalized Arterial Calcification
    Authors: Alexei Y. Savinov, Maryam Salehi, Manisha C. Yadav, Ilian Radichev, José Luis Millán, Olga V. Savinova
    Journal of the American Heart Association
  17. Mesendogen, a novel inhibitor of TRPM6, promotes mesoderm and definitive endoderm differentiation of human embryonic stem cells through alteration of magnesium homeostasis
    Authors: Yijie Geng, Bradley Feng
    Heliyon
  18. Prerequisites for Functional Interleukin 31 Signaling and Its Feedback Regulation by Suppressor of Cytokine Signaling 3 (SOCS3).
    Authors: Maier E, Mittermeir M, Ess S, Neuper T, Schmiedlechner A, Duschl A, Horejs-Hoeck J
    J Biol Chem, 2015-08-25;290(41):24747-59.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  19. Trafficking of LAG-3 to the surface on activated T cells via its cytoplasmic domain and protein kinase C signaling.
    Authors: Bae J, Lee S, Park C, Lee Y, Chun T
    J Immunol, 2014-08-08;193(6):3101-12.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  20. Co-targeting the IGF system and HIF-1 inhibits migration and invasion by (triple-negative) breast cancer cells.
    Authors: Mancini M, Gariboldi M, Taiana E, Bonzi M, Craparotta I, Pagin M, Monti E
    Br J Cancer, 2014-05-22;110(12):2865-73.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry Control
  21. Human mesenchymal stem/stromal cells cultured as spheroids are self-activated to produce prostaglandin E2 that directs stimulated macrophages into an anti-inflammatory phenotype.
    Authors: Ylostalo J, Bartosh T, Coble K, Prockop D
    Stem Cells, 2012-10-01;30(10):2283-96.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Control
  22. Neurotrophins regulate bone marrow stromal cell IL-6 expression through the MAPK pathway.
    Authors: Rezaee F, Rellick SL, Piedimonte G
    PLoS ONE, 2010-03-15;5(3):e9690.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  23. CD4(+)CD25(+) T regulatory cells inhibit CD8(+) IFN-gamma production during acute and chronic FIV infection utilizing a membrane TGF-beta-dependent mechanism.
    Authors: Fogle JE, Mexas AM, Tompkins WA, Tompkins MB
    AIDS Res. Hum. Retroviruses, 2010-02-01;26(2):201-16.
    Species: Feline
    Sample Types: Whole Cells
    Applications: Control
  24. Human Cytomegalovirus interleukin-10 promotes proliferation and migration of MCF-7 breast cancer cells.
    Authors: Bishop R, Valle Oseguera C, Spencer J
    Cancer Cell Microenviron, 2015-01-01;2(1):.

FAQs

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Reviews for Goat IgG PE-conjugated Antibody

Average Rating: 5 (Based on 5 Reviews)

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Goat IgG PE-conjugated Antibody
By Anonymous on 05/12/2022
Application: Flow Sample Tested: Peritoneal resident macrophages Species: Mouse

As a control of Mouse TGF-beta RII PE-conjugated Antibody (Catlog# FAB532P-025UG), it worked well.

Flow Cytometry Goat IgG PE-conjugated Antibody IC108P
Goat IgG PE-conjugated Antibody
By Liang Lin on 09/30/2020
Application: Flow Sample Tested: cell line Species: Human
Flow Cytometry Goat IgG PE-conjugated Antibody IC108P
Goat IgG PE-conjugated Antibody
By Anonymous on 07/26/2020
Application: Flow Sample Tested: Blood mononuclear cells (PBMCs) Species: Human
Flow Cytometry Goat IgG PE-conjugated Antibody IC108P
Goat IgG PE-conjugated Antibody
By Anonymous on 01/10/2020
Application: Flow Sample Tested: T cells Species: Human
Flow Cytometry Goat IgG PE-conjugated Antibody IC108P
Goat IgG PE-conjugated Antibody
By Lucy Quach on 04/02/2018
Application: Flow Sample Tested: HCT-116 human colorectal carcinoma cell line Species: Human
Flow Cytometry Goat IgG PE-conjugated Antibody IC108P