3-Azido-7-hydroxycoumarin
Chemical Name: 3-Azido-7-hydroxy-2H-1-benzopyran-2-one
Purity: ≥95%
Biological Activity
Key information: 3-Azido-7-hydroxycoumarin is a water-soluble fluorogenic fluorescent dye. Supplied with an azide reactive handle for click chemistry. It emits intense fluorescence only after 'click' reaction when it forms triazoles at the site of alkyne modified biomolecules. Suitable for live-cell imaging.Application: Fluorescence microscopy. Can be used to label DNA in proliferating cells following 5-Ethynyl-2'-deoxyuridine (Cat.No. 7207) incorporation. Cell permeable.
Properties and Photophysical Data: Excitation and emission maxima (λ) are 404 nm and 477 nm, respectively, after click reaction.
Optical Data for 3-Azido-7-hydroxycoumarin
| Emission Color | Blue |
|---|---|
| λabs | 404 nm |
| λem | 477 nm |
| Application | Fluorescence Microscopy |
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Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
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