BI dihydrochloride
Chemical Name: (5Z)-3-(1H-Benzimidazol-7-ylmethyl)-5-[(3,5-difluoro-4-hydroxyphenyl)methylene]-3,5-dihydro-2-methyl-4H-imidazol-4-one dihydrochloride
Purity: ≥98%
Biological Activity
BI is a DFHBI derivative for imaging of RNA in cells. BI increases brightness and photostability of Broccoli aptamers in cells. BI enables imaging of single Broccoli-tagged mRNAs in living cells. BI exhibits peak excitation of 470 nm and peak fluorescence emission of 505 nm when bound to Broccoli. BI exhibits higher affinity for Broccoli aptamers and a 10.5-fold increase in cellular fluorescence compared with DFHBI-1T (Cat. No. 5610) (KD values are 51 and 305 nM, respectively).Optical Data for BI dihydrochloride
| Emission Color | Green |
|---|---|
| λabs | 470 nm |
| λem | 505 nm |
| Extinction Coefficient (ε) | 33600 M-1cm-1 |
| Quantum Yield (φ) | 0.669 |
| Cell Permeable | Yes |
| Application | DFHBI Derivative for Imaging of RNA in Living Cells |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
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