Bovine IL-2 Biotinylated Antibody

Catalog #: BAF2465 Datasheet / COA / SDS

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BAF2465

All IL-2 Antibodies

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Bovine IL-2 Biotinylated Antibody Summary

Species Reactivity

Bovine

Specificity

Detects bovine IL-2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human IL-2, recombinant mouse IL-2, recombinant rat IL-2, recombinant feline IL-2, recombinant canine IL-2, recombinant equine IL-2, recombinant cotton rat IL-2, and recombinant porcine IL-2 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant bovine IL‑2
Ala21-Thr155
Accession # P05016

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Bovine IL‑2 (Catalog # 2465-BL)

Bovine IL-2 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Bovine IL‑2 Antibody (Catalog # AF2465)

Standard: Recombinant Bovine IL-2 Protein (Catalog # 2465-BL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-2

Bovine Interleukin-2 (IL-2) is a 15 kDa, alpha -helical, single chain, potentially glycosylated polypeptide that has potent stimulatory activity for antigen-activated T cells (1‑5). The molecule is synthesized as a 155 amino acid (aa) precursor that contains a 20 aa signal peptide plus a 135 aa mature segment that is possibly O‑glycosylated (4, 5). The mature region has multiple alpha -helices and one intrachain disulfide bond. Mature bovine IL-2 is 64%, 60%, 49%, 50%, 72%, 63% and 67% to mature human, canine, mouse, rat, porcine, equine, and feline IL-2, respectively. Mammalian cells known to express IL-2 include CD4⁺ and CD8⁺ T cells, visceral smooth muscle cells, eosinophils, gamma δ T cells, B cells and dendritic cells. The receptor for IL-2 is complex and consists of three distinct subunits in varying combinations (6, 7). Two of these are ligand-binding and are termed IL-2 R alpha and IL-2 R beta. IL-2 R alpha is 55 kDa and binds IL-2 with low affinity. IL-2 R beta is 75 kDa and binds IL-2 with intermediate affinity. Signal transduction is performed by both IL-2 R beta and a 64 kDa common gamma chain ( gamma _c). This signal transducing common gamma chain does not bind IL-2, but does heterodimerize with IL-2 R beta to form a functional IL-2 receptor. The complex heterotrimeric alpha -beta -gamma c receptor may arise from IL-2 binding to preformed R alpha -R beta complexes (8). Functionally, IL-2 is best known for its autocrine and paracrine activity on T cells. It drives resting T cells into active G1, inducing IL-2 and IL-2 R alpha synthesis and cell proliferation (7). It also promotes Fas-induced death of naïve CD4⁺ T cells, while having minimal effect on activated CD4⁺ memory lymphocytes. Finally, IL-2 seems to play a central role in the expansion and maintenance of CD4⁺ CD25⁺ regulatory T cells. Thus, IL-2 may be a key cytokine in the natural suppression of autoimmunity (9, 10).

References

Smith, K.A. (1992) Curr. Opin. Immunol. 4:271.
Smith, K.A. (1988) Science 240:1169.
Waldmann, T.A. et al. (2001) Immunity 14:105.
Cerretti, D.P. et al. (1986) Proc. Natl. Acad. Sci. USA 83:3223.
Reeves, R. et al. (1986) Proc. Natl. Acad. Sci. USA 83:3228.
Ellery, J.M. and P.J. Nicholls (2002) Cytokine Growth Factor Rev. 13:27.
Nelson, B.H. and D.M. Willerford (1998) Adv. Immunol. 70:1.
Liparoto, S.F. et al. (2002) Biochemistry 41:2543.
Jaleco, S. et al. (2003) J. Immunol. 171:61.
Malek, T.R. (2003) J. Leukoc. Biol. 74:961.

Long Name

Interleukin 2

Entrez Gene IDs

3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)

Alternate Names

Aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; Proleukin; T cell growth factor; T-cell growth factor; TCGF