Canine CD44 APC-conjugated Antibody

Name: Canine CD44 APC-conjugated Antibody
Brand: R&D Systems
SKU: FAB5449A
Rating: 5 (1 reviews)

Catalog #: FAB5449A 1 Review Datasheet / COA / SDS

Newer Version Available: FAB5449G

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Discontinued Product

FAB5449A has been discontinued and is replaced by FAB5449G.

Detection of CD44 in Canine Blood-Derived Lymphocytes by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Canine CD44 APC-conjugated Antibody Summary

Species Reactivity

Canine

Specificity

Detects canine CD44.

Source

Monoclonal Mouse IgG₁ Clone # 69-S5

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Canine marrow cells
Accession # Q28284

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD44 in Canine Blood-Derived Lymphocytes by Flow Cytometry. Canine blood-derived lymphocytes were stained with Mouse Anti-Canine CD44 APC-conjugated Monoclonal Antibody (Catalog # FAB5449A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CD44

Canine CD44 (also known as HCAM and Hermes) is a ubiquitously expressed 85‑90 kDa type I transmembrane glycoprotein that binds to hyaluronan, laminin, fibronectin, selectins, osteopontin, and chondroitin sulfate, and is involved in matrix adhesion, lymphocyte activation, and lymph node homing. The CD44 protein is expressed as a family of molecular isoforms that, in human and presumably in canine, are generated by alternative splicing and variable posttranslational modification. It is reported to form a complex with CD147. Within the N‑terminal invariant portion of the ECD (aa 14‑191), canine CD44 shares 90%, 83%, and 82% identity with human, mouse, and rat CD44, respectively.

Entrez Gene IDs

960 (Human); 12505 (Mouse); 25406 (Rat); 100126860 (Porcine)

Alternate Names

CD44 antigen; CD44 molecule (Indian blood group); CD44; CD44R; CDw44; cell surface glycoprotein CD44; chondroitin sulfate proteoglycan 8; CSPG8; ECMR-III; epican; Extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; HCAM; HCELL; hematopoietic cell E- and L-selectin ligand; Heparan sulfate proteoglycan; Hermes antigen; homing function and Indian blood group system; HUTCH-I; Hyaluronate receptor; IN; LHR; MC56; MDU2; MDU2CD44 antigen (homing function and Indian blood group system); MDU3; MDU3CDW44; MIC4; MIC4MGC10468; MUTCH-I; Pgp1; PGP-1; PGP-I; Phagocytic glycoprotein 1; Phagocytic glycoprotein I