Canine IL-21 Antibody Summary
His18-Ser146
Accession # NP_001003347
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
![Cell Proliferation Induced by IL‑21 and Neutralization by Canine IL‑21 Antibody. Cell Proliferation Induced by IL‑21 and Neutralization by Canine IL‑21 Antibody.](https://resources.rndsystems.com/images/datasheets/antibody/IL-21_AF5849_Block_Neutralize_10993.jpg)
Cell Proliferation Induced by IL‑21 and Neutralization by Canine IL‑21 Antibody. Recombinant Canine IL-21 (Catalog # 5849-ML) induces proliferation in the N1186 human T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine IL-21 (10 ng/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Canine IL-21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5849). The ND50 is typically 2-10 µg/mL.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-21
Interleukin-21 (IL-21) is a 14 kDa (predicted) four-helix-bundle cytokine and member of the IL-15/IL-21 family. It is made by activated CD4+ T cells, activated NKT cells, T helper (Th) cells, and Th17 cells (1‑5). Canine IL-21 is synthesized as a 146 amino acid (aa) precursor that contains a 17 aa signal sequence and a 129 aa mature chain. Mature canine IL-21 is 86%, 73%, 66%, and 64% aa identical to mature bovine, human, mouse and rat IL-21, respectively. IL-21 binds to a heterodimeric receptor, which is formed by the common gamma -chain subunit (CD134), shared with IL-2, IL-4, IL-7, IL-9, IL-13, and IL-15 receptors, and its own receptor, IL‑21 R, a member of the class I cytokine receptor family (1‑2). IL-21 R is expressed on a variety of immune cells, including B cells, T cells, dendritic cells, and NK cells, as well as non-immune cells, such as fibroblasts, epithelial cells, and endothelial cells (1‑2, 5). Binding of IL-21 to its receptor leads to the activation of members of the JAK-family protein tyrosine kinases, JAK1 and JAK3, and signal transducer and activator of transcription (Stat) molecules (1). IL-21 regulates activation, proliferation, and survival of both CD4+ T cells and B cells, the functional activity of CD8+ T cells and NK cells, and limits the differentiation of inducible regulatory T cells and counteracts their suppressive properties on effector T cells (1, 5‑6). IL-21 also negatively regulates the maturation and function of dendritic cells (1, 5‑6). IL‑21 also plays a role in many inflammatory and autoimmune diseases, such as Crohn’s disease, Helicobacter pylori‑related gastritis, celiac disease, type I diabetes mellitus, psoriasis, rheumatoid arthritis, and systemic lupus erythematosus (1). IL‑21 is also involved in controlling chronic viral infections (7).
- Monteleone, G. et al. (2009) Cytokine Growth Factor Rev. 20:185.
- Parrish-Novak, J. et al. (2000) Nature 408:57.
- Coquet, J.M. et al. (2007) J. Immunol.178:2827.
- Vogelzang, A. et al. (2008) Immunity 29:127.
- Monteleone, G. et al. (2008) Trends Immunol. 29:290.
- Spolski, R. et al. (2008) Curr. Opin. Immunol. 20:295.
- Yi, J.S. et al. (2009) Science 324:1572.
Product Datasheets
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Canine IL-21 Antibody
There are currently no reviews for this product. Be the first to review Canine IL-21 Antibody and earn rewards!
Have you used Canine IL-21 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image