dTAG Janelia Fluor® 525 New
Chemical Name: 2-(3-(3,3-Difluoroazetidin-1-ium-1-ylidene)-6-(3,3-difluoroazetidin-1-yl)-3H-xanthen-9-yl)-4-((2-(2-(3-((R)-3-(3,4-dimethoxyphenyl)-1-(((S)-1-((S)-2-(3,4,5-trimethoxyphenyl)butanoyl)piperidine-2-carbonyl)oxy)propyl)phenoxy)acetamido)ethyl)carbamoyl)benzoate
Purity: ≥95%
Biological Activity
Key Information: dTAG Janelia Fluor® 525 is a fluorogenic srTAG probe for live cell imaging of FKBP12F36V/L fusion proteins.Application: Suitable for confocal microscopy and Super Resolution Microscopy (SRM) including STED. dTAG Janelia Fluor® 525 is cell permeable.
Properties and Photophysical Data: In FRAP (fluorescence recovery after photobleaching) experiment, the highest maximum recovery ratio (73%) was shown when U2OS cells expressing FKBPF36L were incubated with 10 μM dTAG Janelia Fluor® 525; excitation and emission maxima (λ) are 530 nm and 560 nm, respectively; quantum yield = 0.47; extinction coefficient = 82,800 M-1cm-1 (in 4% TFA).
It is recommended to use a working concentration of 10 μM in live cell imaging application.
Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.
Optical Data for dTAG Janelia Fluor® 525
| Emission Color | Yellow |
|---|---|
| Brightness | |
| λabs | 530 nm |
| λem | 560 nm |
| Extinction Coefficient (ε) | 82800 M-1cm-1 |
| Quantum Yield (φ) | 0.47 |
| Closest Laser line | 488 nm |
| Cell Permeable | Yes |
| Application | Confocal Microscopy, Super Resolution Microscopy |
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Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
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