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Human CCL5/RANTES DuoSet ELISA

Catalog #: DY278
67 Citations 3 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DY278
DY278-05
Ancillary Products Available
DY008B: DuoSet ELISA Ancillary Reagent Kit 2
DY999B: Substrate Reagent Pack
DY999: Substrate Reagent Pack
Human CCL5 / RANTES ELISA Standard Curve
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Product Details
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Citations (67)
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Supplemental Products
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Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Human CCL5/RANTES DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
15.6 - 1,000 pg/mL
Sufficient Materials
For five or fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human CCL5/RANTES. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

 

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data

N/A Human CCL5 ELISA / RANTES ELISA Standard Curve View Larger

Human CCL5 / RANTES ELISA Standard Curve

Product Datasheets

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Product Datasheet
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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL5/RANTES

RANTES (Regulated upon Activation, Normal T cell Expressed and presumably Secreted), also known as CCL5, is a member of the "CC" subfamily of chemokines. It plays a primary role in the inflammatory immune response via its ability to chemoattract leukocytes and modulate their function. The cDNA for RANTES was initially discovered by subtractive hybridization as a T cell specific sequence (1, 2). Human RANTES cDNA encodes a highly basic 91 amino acid (aa) residue precursor polypeptide with a 23 aa hydrophobic signal peptide that is cleaved to generate the 68 aa mature protein (1, 2). Human RANTES exhibits approximately 85% homology with mouse RANTES at the deduced aa level (3, 4). 

RANTES is a potent chemoattractant for a number of different cell types including unstimulated CD4+/CD45RO+ memory T cells and stimulated CD4+ and CD8+ T cells with naive and memory phenotypes, NK cells, basophils, eosinophils, dendritic cells, mast cells, monocytes, and microglia (5-13). In addition to its effects on migration, RANTES can activate a number of cell types including T cells (14-16), monocytes (17), neutrophils (17), NK cells (7), dendritic cells (18), and astrocytes (19). T cell activation generally requires relatively high RANTES concentrations (~ 1 μM) and is dependent upon aggregation of the molecule and association with cell surface glycosaminoglycans (GAGs) (15-17). Whether this activity occurs in vivo remains unclear although in mice, intraperitoneally injected RANTES mutants that are unable to aggregate and/or bind GAG, are not capable of attracting leukocytes when compared to wild-type controls (20). Other in vivo studies show that RANTES knockout mice exhibit deficient recruitment of leukocytes to sites of acute inflammation (21). 
RANTES, is known to interact with four identified seven transmembrane G-protein coupled receptors: CCR1, CCR3, CCR4, and CCR5 (22-25). RANTES stimulation can initiate a variety of signaling cascades that are cell context dependent. For instance, in T-cells, RANTES can stimulate elevations of intracellular Ca2+ (26), and activation of focal adhesion kinase (FAK) (27), protein kinase A (28), PI3-kinase (14), Rho GTPase (29), and JAK/STAT signaling pathways (30). The cytomegalovirus protein US28 exhibits significant homology with CC chemokine receptors and is capable of binding RANTES (31). Membrane-spanning US28 can, depending on the context, signal in a constitutive manner (32), bind RANTES and initiate G-protein-mediated signaling cascades (33), or sequester RANTES and potentially alter inflammatory responses (34-36). 
The RANTES receptor CCR5 is also the primary co-receptor for R5 (M-tropic) variants of HIV-1 (37, 38). It has been demonstrated that RANTES, as well as the other CCR5 ligands, macrophage inflammatory protein (MIP)-1 alpha and MIP-1 beta, can competitively inhibit CCR5/HIV-1 interaction and suppress viral infection in vitro (39, 40). These effects apparently do not require fully intact signaling from the CCR5 receptor (41). Consequently, modified forms of RANTES and non-peptide compounds that block the interaction of HIV-1 with CCR5 show promise for future therapies (41-44). In contrast, several reports show that RANTES can enhance in vitro replication of X4 (T-tropic) variants of HIV-1 that use CXCR4 as a co-receptor rather than CCR5 (45, 46). This activity usually requires relatively high RANTES concentrations (~μM) and is dependent upon interaction with cell surface GAGs, oligomerization, and activation of tyrosine kinase and MAP kinase signaling cascades (46, 47).

Entrez Gene IDs:
6352 (Human); 20304 (Mouse); 403522 (Canine); 493689 (Feline)
Alternate Names:
CCL5; chemokine (C-C motif) ligand 5; D17S136Enormally T-expressed, and presumably secreted; EoCP; Eosinophil chemotactic cytokine; RANTES; SISd; SIS-delta; small inducible cytokine A5 (RANTES); small inducible cytokine subfamily A (Cys-Cys), member 5; Small-inducible cytokine A5; T cell-specific protein P228; T-cell specific protein p288; TCP228T-cell-specific protein RANTES

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

 

Citations for Human CCL5/RANTES DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

67 Citations: Showing 1 - 10
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  1. Effect of compressive force combined with vibration on CCL2 and CCL5 in human periodontal ligament cells
    Authors: Pongtiwattanakul, S;Leethanakul, C;Rattanaporn, O;Thammanichanon, P;Tannukit, S;
    Journal of oral biology and craniofacial research
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Effect of progestin-based contraceptives on HIV-associated vaginal immune biomarkers and microbiome in adolescent girls
    Authors: Nasr, MA;Aldous, A;Daniels, J;Joy, C;Capozzi, E;Yang, M;Moriarty, P;Emmanuel-Baker, V;Malcolm, S;Green, SJ;Gomez-Lobo, V;Ghosh, M;
    PloS one
    Species: Human
    Sample Types: Vaginal Lavage Fluid
  3. Baseline circulating biomarkers, their changes, and subsequent suicidal ideation and depression severity at 6 months: A prospective analysis in patients with mood disorders
    Authors: Lengvenyte, A;Cognasse, F;Hamzeh-Cognasse, H;Sénèque, M;Strumila, R;Olié, E;Courtet, P;
    Psychoneuroendocrinology
    Species: Human
    Sample Types: Plasma
  4. A conformation-locking inhibitor of SLC15A4 with TASL proteostatic anti-inflammatory activity
    Authors: Boeszoermenyi, A;Bernaleau, L;Chen, X;Kartnig, F;Xie, M;Zhang, H;Zhang, S;Delacrétaz, M;Koren, A;Hopp, AK;Dvorak, V;Kubicek, S;Aletaha, D;Yang, M;Rebsamen, M;Heinz, LX;Superti-Furga, G;
    Nature communications
    Species: Human
    Sample Types: Cell Culture Supernates
  5. Impulsivity across severe mental disorders: a cross-sectional study of immune markers and psychopharmacotherapy
    Authors: Hjell, G;Rokicki, J;Szabo, A;Holst, R;Tesli, N;Bell, C;Fischer-Vieler, T;Werner, MCF;Lunding, SH;Ormerod, MBEG;Johansen, IT;Djurovic, S;Ueland, T;Andreassen, OA;Melle, I;Lagerberg, TV;Mørch-Johnsen, L;Steen, NE;Haukvik, UK;
    BMC psychiatry
    Species: Human
    Sample Types: Plasma
  6. Three-Dimensional Breast Cancer Model to Investigate CCL5/CCR1 Expression Mediated by Direct Contact between Breast Cancer Cells and Adipose-Derived Stromal Cells or Adipocytes
    Authors: Watzling, M;Klaus, L;Weidemeier, T;Horder, H;Ebert, R;Blunk, T;Bauer-Kreisel, P;
    Cancers
    Species: Human
    Sample Types: Cell Culture Supernates
  7. Mild Cognitive Impairment Is Associated with Enhanced Activation of Th17 Lymphocytes in Non-Alcoholic Fatty Liver Disease
    Authors: Fiorillo, A;Gallego, JJ;Casanova-Ferrer, F;Giménez-Garzó, C;Urios, A;Ballester, MP;Durbán, L;Rios, MP;Megías, J;San Miguel, T;Kosenko, E;Escudero-García, D;Benlloch, S;Felipo, V;Montoliu, C;
    International journal of molecular sciences
    Species: Human
    Sample Types: Plasma
  8. The Endogenous Dual Retinoid Receptor Agonist Alitretinoin Exhibits Immunoregulatory Functions on Antigen-Presenting Cells
    Authors: Kislat, A;Olah, P;Kuchner, M;Gerber, PA;Schrader, J;Meller, S;Homey, B;
    International journal of molecular sciences
    Species: Human
    Sample Types: Cell Culture Supernates
  9. Salmonella Type III Secretion Effector SrfJ: A Glucosylceramidase Affecting the Lipidome and the Transcriptome of Mammalian Host Cells
    Authors: Aguilera-Herce, J;Panadero-Medianero, C;S�nchez-Romero, MA;Balbont�n, R;Bernal-Bayard, J;Ramos-Morales, F;
    International journal of molecular sciences
    Species: Human
    Sample Types: Cell Culture Supernates
  10. MYC promotes immune-suppression in triple-negative breast cancer via inhibition of interferon signaling
    Authors: D Zimmerli, CS Brambillas, F Talens, J Bhin, R Linstra, L Romanens, A Bhattachar, SEP Joosten, AM Da Silva, N Padrao, MD Wellenstei, K Kersten, M de Boo, M Roorda, L Henneman, R de Bruijn, S Annunziato, E van der Bu, AP Drenth, C Lutz, T Endres, M van de Ven, M Eilers, L Wessels, KE de Visser, W Zwart, RSN Fehrmann, MATM van Vugt, J Jonkers
    Nature Communications, 2022-11-02;13(1):6579.
    Species: Human
    Sample Types: Cell Culture Supernates
  11. Development of an Electrochemical CCL5 Chemokine Immunoplatform for Rapid Diagnosis of Multiple Sclerosis
    Authors: S Guerrero, E Sánchez-Ti, L Agüí, A González-C, P Yáñez-Sede, JM Pingarrón
    Biosensors, 2022-08-07;12(8):.
    Species: Human
    Sample Types: Serum
  12. RANTES and CD40L under Conditions of Long-Term Physical Exercise: A Potential Link to Adaptive Immunity
    Authors: M Lenz, R Schönbauer, S Stojkovic, J Lee, C Gatterer, M Lichtenaue, V Paar, M Emich, M Fritzer-Sz, J Strametz-J, S Graf, M Sponder
    Oncogene, 2022-07-16;19(14):.
    Species: Human
    Sample Types: Whole Blood
  13. Th1 cytokines synergize to change gene expression and promote corticosteroid insensitivity in pediatric airway smooth muscle
    Authors: D Jackson, J Walum, P Banerjee, BW Lewis, YS Prakash, V Sathish, Z Xu, RD Britt
    Respiratory Research, 2022-05-16;23(1):126.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. Blockade of Autocrine CCL5 Responses Inhibits Zika Virus Persistence and Spread in Human Brain Microvascular Endothelial Cells
    Authors: MC Mladinich, JN Conde, WR Schutt, SY Sohn, ER Mackow
    MBio, 2021-08-17;12(4):e0196221.
    Species: Human
    Sample Types: Cell Culture Supernates
  15. Rapid Internalization and Nuclear Translocation of CCL5 and CXCL4 in Endothelial Cells
    Authors: A Dickhout, DM Kaczor, ACA Heinzmann, SLN Brouns, JWM Heemskerk, MAMJ van Zandvo, RR Koenen
    International Journal of Molecular Sciences, 2021-07-08;22(14):.
    Species: Human
    Sample Types: Cell Lysates
  16. Adipocytokines in Untreated Newly Diagnosed Rheumatoid Arthritis: Association with Circulating Chemokines and Markers of Inflammation
    Authors: GK Vasileiadi, AC Lundell, Y Zhang, K Andersson, I Gjertsson, A Rudin, C Maglio
    Biomolecules, 2021-02-21;11(2):.
    Species: Human
    Sample Types: Plasma
  17. Resveratrol treatment reduces expression of MCP-1, IL-6, IL-8 and RANTES in endometriotic stromal cells
    Authors: R Kolahdouz-, F Shidfar, S Khodaverdi, T Arablou, S Heidari, N Rashidi, AA Delbandi
    Journal of Cellular and Molecular Medicine, 2020-12-15;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  18. Clinical Relevance of the Anti-inflammatory Effects of Roflumilast on Human Bronchus: Potentiation by a Long-Acting Beta-2-Agonist
    Authors: H Salvator, A Buenestado, M Brollo, E Naline, T Victoni, E Longchamp, H Tenor, S Grassin-De, P Devillier
    Frontiers in Pharmacology, 2020-12-08;11(0):598702.
    Species: Human
    Sample Types: Cell Culture Supernates
  19. TASL is the SLC15A4-associated adaptor for IRF5 activation by TLR7-9
    Authors: LX Heinz, J Lee, U Kapoor, F Kartnig, V Sedlyarov, K Papakostas, A César-Razq, P Essletzbic, U Goldmann, A Stefanovic, JW Bigenzahn, S Scorzoni, MD Pizzagalli, A Bensimon, AC Müller, FJ King, J Li, E Girardi, ML Mbow, CE Whitehurst, M Rebsamen, G Superti-Fu
    Nature, 2020-05-13;581(7808):316-322.
    Species: Human
    Sample Types: Cell Culture Supernates
  20. Human BCL-G regulates secretion of inflammatory chemokines but is dispensable for induction of apoptosis by IFN-gamma and TNF-alpha in intestinal epithelial cells
    Authors: JA Woznicki, P Flood, M Bustamante, P Stamou, G Moloney, A Fanning, SA Zulquernai, J McCarthy, F Shanahan, S Melgar, K Nally
    Cell Death Dis, 2020-01-27;11(1):68.
    Species: Human
    Sample Types: Cell Culture Supernates
  21. HIV and HCV augments inflammatory responses through increased TREM-1 expression and signaling in Kupffer and Myeloid cells
    Authors: J Hyun, RS McMahon, AL Lang, JS Edwards, AD Badilla, ME Greene, GW Stone, S Pallikkuth, M Stevenson, DM Dykxhoorn, S Kottilil, S Pahwa, E Thomas
    PLoS Pathog., 2019-07-01;15(7):e1007883.
    Species: Human
    Sample Types: Cell Culture Supernates
  22. Bone marrow-derived mesenchymal stem cells promote colorectal cancer progression via CCR5
    Authors: G Nishikawa, K Kawada, J Nakagawa, K Toda, R Ogawa, S Inamoto, R Mizuno, Y Itatani, Y Sakai
    Cell Death Dis, 2019-03-19;10(4):264.
    Species: Human
    Sample Types: Serum
  23. Toll-like receptor 3 regulates Zika virus infection and associated host inflammatory response in primary human astrocytes
    Authors: CR Ojha, M Rodriguez, MKM Karuppan, J Lapierre, F Kashanchi, N El-Hage
    PLoS ONE, 2019-02-08;14(2):e0208543.
    Species: Human
    Sample Types: Cell Culture Supernates
  24. Tick-Borne Encephalitis Virus Nonstructural Protein NS5 Induces RANTES Expression Dependent on the RNA-Dependent RNA Polymerase Activity
    Authors: Z Zheng, J Yang, X Jiang, Y Liu, X Zhang, M Li, M Zhang, M Fu, K Hu, H Wang, MH Luo, P Gong, Q Hu
    J. Immunol., 2018-05-14;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  25. Genetic Polymorphism at CCL5 Is Associated With Protection in Chagas' Heart Disease: Antagonistic Participation of CCR1+ and CCR5+ Cells in Chronic Chagasic Cardiomyopathy.
    Authors: Angelica Martins Batista, Lucia Elena Alvarado-, Silvia Marinho Alves, Gloria Melo, Isabela Resende Pereira, Leonardo Alexandre de Sou Ruivo, Andrea Alice Da Silva, Daniel Gibaldi, Thayse Do E S Protásio Da Silva, Virginia Maria Barros De Lorena, Adriene Siqueira De Melo, Ana Karine De Araújo, Michelle Da Silva Barros, Vláudia Maria Assis Costa, Cynthia C Cardoso, Antonio G Pacheco, Cristina Carrazzon, Wilson Oliveira, Milton Ozório Moraes, Joseli Lannes-Vi
    Frontiers in Immunology, 2018-04-11;0(0):1664-3224.
    Species: Human
    Sample Types: Serum
  26. The effects of repeated Toll-like receptors 2 and 4 stimulation in COPD alveolar macrophages
    Authors: SR Lea, SL Reynolds, M Kaur, KD Simpson, SR Hall, EM Hessel, D Singh
    Int J Chron Obstruct Pulmon Dis, 2018-03-02;13(0):771-780.
    Species: Human
    Sample Types: Cell Culture Supernates
  27. Pivotal role for the ESCRT-II complex subunit EAP30/SNF8 in IRF3-dependent innate antiviral defense
    Authors: K Kumthip, D Yang, NL Li, Y Zhang, M Fan, A Sethuraman, K Li
    PLoS Pathog., 2017-10-30;13(10):e1006713.
    Species: Human
    Sample Types: Cell Culture Supernates
  28. Zika Virus Persistently Infects and Is Basolaterally Released from Primary Human Brain Microvascular Endothelial Cells
    Authors: MC Mladinich, J Schwedes, ER Mackow
    MBio, 2017-07-11;8(4):.
    Species: Human
    Sample Types: Cell Culture Supernates
  29. Malaria pigment stimulates chemokine production by human microvascular endothelium
    Authors: N Basilico, Y Corbett, S Alessandro, S Parapini, M Prato, D Girelli, P Misiano, P Olliaro, D Taramelli
    Acta Trop., 2017-05-02;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  30. IFI16 and cGAS cooperate in the activation of STING during DNA sensing in human keratinocytes
    Authors: JF Almine, CA O'Hare, G Dunphy, IR Haga, RJ Naik, A Atrih, DJ Connolly, J Taylor, IR Kelsall, AG Bowie, PM Beard, L Unterholzn
    Nat Commun, 2017-02-13;8(0):14392.
    Species: Human
    Sample Types: Cell Culture Supernates
  31. Mitochondrial proteins NIP-SNAP-1 and -2 are a target for the immunomodulatory activity of clarithromycin, which involves NF-?B-mediated cytokine production
    Authors: Soh Yamamoto
    Biochem. Biophys. Res. Commun, 2016-12-18;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  32. Forsythia suspensa Suppresses House Dust Mite Extract-Induced Atopic Dermatitis in NC/Nga Mice
    PLoS ONE, 2016-12-09;11(12):e0167687.
    Species: Human
    Sample Types: Cell Culture Supernates
  33. A Role for Human Skin Mast Cells in Dengue Virus Infection and Systemic Spread
    Authors: Tonya M Colpitts
    J. Immunol., 2016-10-31;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  34. Inflammatory and immunological profiles in patients with COPD: relationship with FEV 1 reversibility
    Authors: Cleriston Farias Queiroz
    J Bras Pneumol, 2016-07-01;42(4):241-247.
    Species: Human
    Sample Types: Serum
  35. Unfractionated Heparin Selectively Modulates the Expression of CXCL8, CCL2 and CCL5 in Endometrial Carcinoma Cells
    Authors: A Doster, U Schwarzig, M Zygmunt, J Rom, F Schütz, H Fluhr
    Anticancer Res, 2016-04-01;36(4):1535-44.
    Species: Human
    Sample Types: Cell Culture Supernates
  36. Experimental hyperglycemia induces an increase of monocyte and T-lymphocyte content in adipose tissue of healthy obese women.
    Authors: Tencerova M, Kracmerova J, Krauzova E, Malisova L, Kovacova Z, Wedellova Z, Siklova M, Stich V, Rossmeislova L
    PLoS ONE, 2015-04-20;10(3):e0122872.
    Species: Human
    Sample Types: Plasma
  37. Role of hydrogen sulfide in paramyxovirus infections.
    Authors: Li H, Ma Y, Escaffre O, Ivanciuc T, Komaravelli N, Kelley J, Coletta C, Szabo C, Rockx B, Garofalo R, Casola A
    J Virol, 2015-03-04;89(10):5557-68.
    Species: Human
    Sample Types: Cell Culture Supernates
  38. Necroptosis suppresses inflammation via termination of TNF- or LPS-induced cytokine and chemokine production.
    Authors: Kearney C, Cullen S, Tynan G, Henry C, Clancy D, Lavelle E, Martin S
    Cell Death Differ, 2015-01-23;22(8):1313-27.
    Species: Human
    Sample Types: Cell Culture Supernates
  39. Resolving cancer-stroma interfacial signalling and interventions with micropatterned tumour-stromal assays.
    Authors: Shen K, Luk S, Hicks D, Elman J, Bohr S, Iwamoto Y, Murray R, Pena K, Wang F, Seker E, Weissleder R, Yarmush M, Toner M, Sgroi D, Parekkadan B
    Nat Commun, 2014-12-09;5(0):5662.
    Species: Human
    Sample Types: Cell Culture Supernates
  40. Human osteoarthritic cartilage shows reduced in vivo expression of IL-4, a chondroprotective cytokine that differentially modulates IL-1beta-stimulated production of chemokines and matrix-degrading enzymes in vitro.
    Authors: Assirelli E, Pulsatelli L, Dolzani P, Platano D, Olivotto E, Filardo G, Trisolino G, Facchini A, Borzi R, Meliconi R
    PLoS ONE, 2014-05-12;9(5):e96925.
    Species: Human
    Sample Types: Cell Culture Supernates
  41. Controlled intramyocardial release of engineered chemokines by biodegradable hydrogels as a treatment approach of myocardial infarction.
    Authors: Projahn D, Simsekyilmaz S, Singh S, Kanzler I, Kramp B, Langer M, Burlacu A, Bernhagen J, Klee D, Zernecke A, Hackeng T, Groll J, Weber C, Liehn E, Koenen R
    J Cell Mol Med, 2014-02-06;18(5):790-800.
    Species: Human
    Sample Types: Cell Culture Supernates
  42. Proteome-wide analysis and CXCL4 as a biomarker in systemic sclerosis
    Authors: L van Bon, AJ Affandi, J Broen, RB Christmann, RJ Marijnisse, L Stawski, GA Farina, G Stifano, AL Mathes, M Cossu, M York, C Collins, M Wenink, R Huijbens, R Hesselstra, T Saxne, M DiMarzio, D Wuttge, SK Agarwal, JD Reveille, S Assassi, M Mayes, Y Deng, JP Drenth, J de Graaf, M den Heijer, CG Kallenberg, M Bijl, A Loof, WB van den Be, LA Joosten, V Smith, F de Keyser, R Scorza, C Lunardi, PL van Riel, M Vonk, W van Heerde, S Meller, B Homey, L Beretta, M Roest, M Trojanowsk, R Lafyatis, TR Radstake
    N. Engl. J. Med, 2013-12-18;370(5):433-43.
    Species: Human
    Sample Types: Plasma
  43. The small GTPase Arf6 is essential for the Tram/Trif pathway in TLR4 signaling.
    Authors: Van Acker T, Eyckerman S, Vande Walle L, Gerlo S, Goethals M, Lamkanfi M, Bovijn C, Tavernier J, Peelman F
    J Biol Chem, 2013-12-02;289(3):1364-76.
    Species: Human
    Sample Types: Cell Culture Supernates
  44. 1,25-dihydroxyvitamin D3 protects against macrophage-induced activation of NFkappaB and MAPK signalling and chemokine release in human adipocytes.
    Authors: Ding C, Wilding J, Bing C
    PLoS ONE, 2013-04-24;8(4):e61707.
    Species: Human
    Sample Types: Cell Culture Supernates
  45. Human cytomegalovirus induces a biphasic inflammatory response in primary endothelial cells.
    Authors: Jeffery H, Soderberg-Naucler C, Butler L
    J Virol, 2013-03-27;87(11):6530-5.
    Species: Human
    Sample Types: Cell Culture Supernates
  46. Intradermal delivery of TLR agonists in a human explant skin model: preferential activation of migratory dendritic cells by polyribosinic-polyribocytidylic acid and peptidoglycans.
    Authors: Oosterhoff D, Heusinkveld M, Lougheed S, Kosten I, Lindstedt M, Bruijns S, van Es T, van Kooyk Y, van der Burg S, de Gruijl T
    J Immunol, 2013-03-06;190(7):3338-45.
    Species: Human
    Sample Types: Cell Culture Supernates
  47. Characterisation of P2Y(12) receptor responsiveness to cysteinyl leukotrienes.
    Authors: Foster H, Fuerst E, Lee T, Cousins D, Woszczek G
    PLoS ONE, 2013-03-05;8(3):e58305.
    Species: Human
    Sample Types: Plasma
  48. Measles virus causes immunogenic cell death in human melanoma.
    Authors: Donnelly, O G, Errington-Mais, F, Steele, L, Hadac, E, Jennings, V, Scott, K, Peach, H, Phillips, R M, Bond, J, Pandha, H, Harrington, K, Vile, R, Russell, S, Selby, P, Melcher, A A
    Gene Ther, 2011-12-15;20(1):7-15.
    Species: Human
    Sample Types: Cell Culture Supernates
  49. IRF5 promotes inflammatory macrophage polarization and T(H)1-T(H)17 responses.
    Authors: Krausgruber T, Blazek K, Smallie T
    Nat. Immunol., 2011-01-16;12(3):231-8.
    Species: Human
    Sample Types: Cell Culture Supernates
  50. Thrombocytopenia in early malaria is associated with GP1b shedding in absence of systemic platelet activation and consumptive coagulopathy.
    Authors: de Mast Q, de Groot PG, van Heerde WL, Roestenberg M, van Velzen JF, Verbruggen B, Roest M, McCall M, Nieman AE, Westendorp J, Syafruddin D, Fijnheer R, van Dongen-Lases EC, Sauerwein RW, van der Ven AJ
    Br. J. Haematol., 2010-10-19;151(5):495-503.
    Species: Human
    Sample Types: Plasma
  51. A role for the human nucleotide-binding domain, leucine-rich repeat-containing family member NLRC5 in antiviral responses.
    Authors: Neerincx A, Lautz K, Menning M, Kremmer E, Zigrino P, Hosel M, Buning H, Schwarzenbacher R, Kufer TA
    J. Biol. Chem., 2010-06-10;285(34):26223-32.
    Species: Human
    Sample Types: Cell Culture Supernates
  52. IL-17 amplifies human contact hypersensitivity by licensing hapten nonspecific Th1 cells to kill autologous keratinocytes.
    Authors: Pennino D, Eyerich K, Scarponi C, Carbone T, Eyerich S, Nasorri F, Garcovich S, Traidl-Hoffmann C, Albanesi C, Cavani A
    J. Immunol., 2010-03-31;184(9):4880-8.
    Species: Human
    Sample Types: Cell Culture Supernates
  53. Novel markers of inflammation identified in tumor necrosis factor receptor-associated periodic syndrome (TRAPS) by transcriptomic analysis of effects of TRAPS-associated tumor necrosis factor receptor type I mutations in an endothelial cell line.
    Authors: Rebelo SL, Amel-Kashipaz MR, Radford PM, Bainbridge SE, Fiets R, Fang J, McDermott EM, Powell RJ, Todd I, Tighe PJ
    Arthritis Rheum., 2009-01-01;60(1):269-80.
    Species: Human
    Sample Types: Cell Culture Supernates
  54. CD8 chemokine receptors in chronic obstructive pulmonary disease.
    Authors: Smyth LJ, Starkey C, Gordon FS, Vestbo J, Singh D
    Clin. Exp. Immunol., 2008-08-22;154(1):56-63.
    Species: Human
    Sample Types: BALF
  55. Altered levels of CC chemokines during pulmonary CMV predict BOS and mortality post-lung transplantation.
    Authors: Weigt SS, Elashoff RM, Keane MP, Strieter RM, Gomperts BN, Xue YY, Ardehali A, Gregson AL, Kubak B, Fishbein MC, Saggar R, Ross DJ, Lynch JP, Zisman DA, Belperio JA
    Am. J. Transplant., 2008-07-01;8(7):1512-22.
    Species: Human
    Sample Types: BALF
  56. Vitamin D and glucocorticoids differentially modulate chemokine expression in human airway smooth muscle cells.
    Authors: Banerjee A, Damera G, Bhandare R, Gu S, Lopez-Boado Y, Panettieri R, Tliba O
    Br. J. Pharmacol., 2008-06-16;155(1):84-92.
    Species: Human
    Sample Types: Cell Culture Supernates
  57. Expanded-polyglutamine huntingtin protein suppresses the secretion and production of a chemokine (CCL5/RANTES) by astrocytes.
    Authors: Chou SY, Weng JY, Lai HL, Liao F, Sun SH, Tu PH, Dickson DW, Chern Y
    J. Neurosci., 2008-03-26;28(13):3277-90.
    Species: Human
    Sample Types: Cell Culture Supernates
  58. Preadipocyte response and impairment of differentiation in an inflammatory environment.
    Authors: Poulain-Godefroy O, Froguel P
    Biochem. Biophys. Res. Commun., 2007-03-19;356(3):662-7.
    Species: Human
    Sample Types: Cell Culture Supernates
  59. PAPP-A and osteoprotegerin, together with interleukin-8 and RANTES, are elevated in the peritoneal fluid of women with endometriosis.
    Authors: Bersinger NA, von Roten S, Wunder DM, Raio L, Dreher E, Mueller MD
    Am. J. Obstet. Gynecol., 2006-04-25;195(1):103-8.
    Species: Human
    Sample Types: Peritoneal Fluid
  60. The cytokine IL-1beta activates IFN response factor 3 in human fetal astrocytes in culture.
    Authors: Rivieccio MA, John GR, Song X, Suh HS, Zhao Y, Lee SC, Brosnan CF
    J. Immunol., 2005-03-15;174(6):3719-26.
    Species: Human
    Sample Types: Cell Culture Supernates
  61. Manipulation of host cytokine network by ticks: a potential gateway for pathogen transmission.
    Authors: Hajnicka V, Vancova I, Kocakova P, Slovak M, Gasperik J, Slavikova M, Hails RS, Labuda M, Nuttall PA
    Parasitology, 2005-03-01;130(0):333-42.
    Species: Human
    Sample Types: Saliva
  62. Involvement of Fc gamma RI (CD64) in the mechanism of HIV-1 inhibition by polyclonal IgG purified from infected patients in cultured monocyte-derived macrophages.
    Authors: Holl V, Hemmerter S, Burrer R, Schmidt S, Bohbot A, Aubertin AM, Moog C
    J. Immunol., 2004-11-15;173(10):6274-83.
    Species: Human
    Sample Types: Cell Culture Supernates
  63. Rapid chemokine secretion from endothelial cells originates from 2 distinct compartments.
    Authors: Oynebraten I, Bakke O, Brandtzaeg P, Johansen FE, Haraldsen G
    Blood, 2004-03-25;104(2):314-20.
    Species: Human
    Sample Types: Cell Culture Supernates
  64. Virally stimulated plasmacytoid dendritic cells produce chemokines and induce migration of T and NK cells.
    Authors: Megjugorac NJ, Young HA, Amrute SB, Olshalsky SL, Fitzgerald-Bocarsly P
    J. Leukoc. Biol., 2004-01-23;75(3):504-14.
    Species: Human
    Sample Types: Cell Culture Supernates
  65. Chemokine secretion of rheumatoid arthritis synovial fibroblasts stimulated by Toll-like receptor 2 ligands.
    Authors: Pierer M, Rethage J, Seibl R, Lauener R, Brentano F, Wagner U, Hantzschel H, Michel BA, Gay RE, Gay S, Kyburz D
    J. Immunol., 2004-01-15;172(2):1256-65.
    Species: Human
    Sample Types: Cell Culture Supernates
  66. Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS.
    Authors: Giunti D, Borsellino G, Benelli R, Marchese M, Capello E, Valle MT, Pedemonte E, Noonan D, Albini A, Bernardi G, Mancardi GL, Battistini L, Uccelli A
    J. Leukoc. Biol., 2003-05-01;73(5):584-90.
    Species: Human
    Sample Types: CSF
  67. Differential production of RANTES and MCP-1 in synovial fluid from the inflamed human knee.
    Authors: Conti P, Reale M, Barbacane RC, Castellani ML, Orso C
    Immunol. Lett., 2002-02-01;80(2):105-11.
    Species: Human
    Sample Types: Serum

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Human CCL5/RANTES DuoSet ELISA
By Teresa Williams on 12/14/2020
Sample Tested: Cell culture supernatant
Human CCL5/RANTES DuoSet ELISA DY278
Human CCL5/RANTES DuoSet ELISA
By Anonymous on 09/11/2019
Sample Tested: Mouse macrophages
Human CCL5/RANTES DuoSet ELISA
By Anonymous on 07/17/2018
Sample Tested: EDTA Plasma
Human CCL5/RANTES DuoSet ELISA DY278