ErbB2/Her2 (Research Grade Trastuzumab
Biosimilar) Biotinylated Antibody

Detection of ErbB2/Her2 in MCF-7 human cell line by Flow Cytometry. MCF-7 human cell line was stained with Biotinylated Human Anti-Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Monoclonal Antibody (Catalog # FAB9589B, filled histogram) or irrelevant antibody (open histogram) followed by APC-conjugated Streptavidin (Catalog # F0050). View our protocol for Staining Membrane-associated Proteins.

ErbB2/Her2 in SK-BR-3 Human Cell Line. ErbB2/Her2 was detected in both live and fixed SK‑BR‑3 human breast cancer cell line and but not detected in fixed U937 human histiocytic lymphoma cell line. SK-BR-3 live cells were incubated for 1 hour at 4°C with Anti-Human ErbB2/Her2 Biotinylated Monoclonal Antibody (FAB9589B) diluted at 5 µg/mL with HBSS. SK-BR-3 and U937 fixed cells were incubated for 2 hours at room temperature with Anti-Human ErbB2/Her2 Biotinylated Monoclonal Antibody (FAB9589B) diluted with antibody diluent. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; NL999) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.