Feline IL-2 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF1890
IL‑2 in Feline PBMCs.
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Feline IL-2 Biotinylated Antibody Summary

Species Reactivity
Feline
Specificity
Detects feline IL‑2 in ELISAs and Western blots. In sandwich immunoassays, approximately 3% cross‑reactivity with recombinant canine IL-2 is observed, approximately 1% cross-reactivity with recombinant porcine IL-2 and recombinant human IL-2 is observed, and less than 0.2% cross‑reactivity with recombinant mouse IL-2, recombinant equine IL-2, recombinant bovine IL-2, recombinant rat IL-2, and recombinant cotton rat IL-2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant feline IL‑2
Ala21-Thr154 (Cys146Ser)
Accession # Q07885
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Feline IL‑2 (Cys146Ser) (Catalog # 1890-FL)
Immunocytochemistry
5-15 µg/mL
See below

Feline IL-2 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Feline IL‑2 Antibody (Catalog # AF1890)

Standard: Recombinant Feline IL-2 (Cys146Ser) Protein (Catalog # 1890-FL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry IL-2 antibody in Feline PBMCs by Immunocytochemistry (ICC). View Larger

IL‑2 in Feline PBMCs. IL-2 was detected in immersion fixed feline peripheral blood mononuclear cells (PBMCs) using Goat Anti-Feline IL-2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1890) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-2

Interleukin-2 (IL-2) is a secreted, single chain alpha -helical polypeptide that has potent stimulatory activity for antigen-activated T cells. The feline IL-2 gene encodes a 154 amino acid (aa) precursor protein with a 20 aa signal peptide plus a 134 aa mature segment. There are suggestions that the mature protein may be O‑glycosylated. At the aa sequence level, mature feline IL-2 is 78%, 82%, 60%, 64%, 62%, 75%, 62%, and 76% identical to mature human, canine, mouse, rat, cotton rat, porcine, goat, and equine IL-2, respectively. Mammalian cells known to express IL-2 include CD4+ and CD8+ T cells, visceral smooth muscle cells, eosinophils, gamma δ T cells, B cells and dendritic cells. The biological activity of IL-2 is mediated by IL-2 receptor complexes consisting of three distinct subunits ( alpha, beta, gamma ) in two combinations. The high-affinity signaling IL-2 receptor complex is a heterotrimer of the IL-2 receptor alpha, beta, gamma  subunits. The intermediate signaling complex is a heterodimer of the IL-2 R beta  and gamma  subunits. The non-ligand binding   gamma  subunit, referred to as the common gamma  subunit ( gamma c), is also a subunit of the receptor complexes of IL-4, IL-7, IL-9 and IL-15. Functionally, IL-2 is best known for its autocrine and paracrine activity on T cells. On naïve CD8+ T cells, high IL-2 levels can induce cell proliferation with a bias towards cytotoxicity. In the presence of low levels of IL-2, CD8+ T cells preferentially undergo apoptosis with a bias towards cytokine secretion. IL-2 also seems to play a central role in the expansion and maintenance of CD4+ CD25+ regulatory T cells. This indicates IL-2 may be a key cytokine in the natural suppression of autoimmunity (1‑9).

References
  1. Cozzi, P.J. et al. (1993) Biochem. Biophys. Res. Commun. 194:1038.
  2. Smith, K.A. (1992) Curr. Opin. Immunol. 4:271.
  3. Conradt, H.S. et al. (1989) J. Biol. Chem. 264:17368.
  4. Matesanz, F. et al. (1993) Immunogenetics 38:300.
  5. Ellery, J.M. and P.J. Nicholls (2002) Cytokine Growth Factor Rev. 13:27.
  6. Liparoto, S.F. et al. (2002) Biochemistry 41:2543.
  7. Lin, J-X. and W.J. Leonard (1997) Cytokine Growth Factor Rev. 8:313.
  8. Sad, S. and L. Krishnan (1999) J. Immunol. 163:2443.
  9. Malek, T.R. (2003) J. Leukoc. Biol. 74:961.
Long Name
Interleukin 2
Entrez Gene IDs
3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)
Alternate Names
Aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; Proleukin; T cell growth factor; T-cell growth factor; TCGF

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