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Goat Anti-Rat IgG Biotinylated Antibody

Catalog #: BAF005
4 Citations Datasheet / COA / SDS
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BAF005
R&D Systems Antibodies
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Citations (4)
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Goat Anti-Rat IgG Biotinylated Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat IgG1, IgG2A, IgG2B heavy and light chains. In direct ELISAs, less 10% cross-reactivity with mouse IgG and less than 1% cross‑reactivity with human IgG and rabbit IgG is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Rat IgG
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
Use at a 1:5000 dilution
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; Immunoglobulin G; ImmunoglobulinG

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Citations for Goat Anti-Rat IgG Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Tumour stroma-derived Lipocalin-2 promotes breast cancer metastasis
    Authors: B Ören, J Urosevic, C Mertens, J Mora, M Guiu, RR Gomis, A Weigert, T Schmid, S Grein, B Brüne, M Jung
    J Pathol, 2016-04-29;0(0):.
  2. TRPC6 inactivation does not affect loss of renal function in nephrotoxic serum glomerulonephritis in rats, but reduces severity of glomerular lesions
    Authors: EY Kim, PY Shotorbani, SE Dryer
    Biochem Biophys Rep, 2019-01-08;17(0):139-150.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  3. Immune complexes regulate bone metabolism through FcRgamma signalling.
    Authors: Negishi-Koga T, Gober H, Sumiya E, Komatsu N, Okamoto K, Sawa S, Suematsu A, Suda T, Sato K, Takai T, Takayanagi H
    Nat Commun, 2015-03-31;6(0):6637.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Galectin-3 negatively regulates the frequency and function of CD4(+) CD25(+) Foxp3(+) regulatory T cells and influences the course of Leishmania major infection.
    Authors: Fermino M, Dias F, Lopes C, Souza M, Cruz A, Liu F, Chammas R, Roque-Barreira M, Rabinovich G, Bernardes E
    Eur J Immunol, 2013-05-17;43(7):1806-17.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Secondary Antibody

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