Key Product Details

Species Reactivity

Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all IgG Secondary Antibodies »

Product Specifications

Immunogen

Rat IgG

Specificity

Detects rat IgG1, IgG2A, IgG2B heavy and light chains.  In direct ELISA, less than 10% cross-reactivity with mouse IgG and less than 1% cross-reactivity with human IgG and rabbit IgG is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Goat Anti-Rat IgG Antibody

Application
Recommended Usage

Western Blot

Use at a 1:5000 dilution in conjunction with an appropriate tertiary detection antibody

Reviewed Applications

Read 1 review rated 4 using AF005 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Additional IgG Products

Product Documents for Goat Anti-Rat IgG Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Goat Anti-Rat IgG Antibody

For research use only

Citations for Goat Anti-Rat IgG Antibody

Customer Reviews for Goat Anti-Rat IgG Antibody (1)

4 out of 5
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  • Goat Anti-Rat IgG Antibody
    Name: Danlee Enzler
    Application: Immunohistochemistry
    Sample Tested: Cancer Tissue
    Species: Rat
    Verified Customer | Posted 05/01/2018

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FAQs

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