Human A33 Antibody

Detection of Human A33 by Western Blot. Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cells. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human A33 Monoclonal Antibody (Catalog # MAB30804) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for A33 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of A33 in Colo205 cells by Flow Cytometry. Colo205 cells were stained with Rabbit Anti-Human A33 Monoclonal Antibody (Catalog # MAB30804, Filled histogram) or isotype control antibody (Catalog # MAB1050, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins.

Detection of A33 in Human Colon. A33 was detected in immersion fixed paraffin-embedded sections of Human Colon using Rabbit Anti-Human A33 Monoclonal Antibody (Catalog # MAB30804) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Human A33 by Simple Western^TM. Simple Western lane view shows lysates of human colon, loaded at 0.5 mg/ml. A specific band was detected for A33 at approximately 59 kDa (as indicated) using 20 µg/mL of Rabbit Anti-Human A33 Monoclonal Antibody (MAB30804). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.