Human A33 Antibody

Detection of Human A33 by Western Blot. Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human A33 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3080) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for A33 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of A33 in HT‑29 Human Cell Line by Flow Cytometry. HT‑29 human colon carcinoma cell line was stained with Human A33 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3080, filled histogram) or isotype control antibody (AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (F0107).

A33 in Human Colon. A33 was detected in immersion fixed paraffin-embedded sections of human colon using 1.7 µg/mL Goat Anti-Human A33 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3080) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human A33 by Simple Western^TM. Simple Western lane view shows lysates of COLO 205 human colorectal adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for A33 at approximately 64 kDa (as indicated) using 20 µg/mL of Goat Anti-Human A33 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3080). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.