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Human ACE-2 DuoSet ELISA

Catalog #: DY933-05
11 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DY933-05
Ancillary Products Available
DY008B: DuoSet ELISA Ancillary Reagent Kit 2
DY999B: Substrate Reagent Pack
DY999: Substrate Reagent Pack
Human ACE-2 Standard Curve
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Product Details
Citations (11)
FAQs
Supplemental Products
Reviews
Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Human ACE-2 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
0.3 - 20 ng/mL
Sufficient Materials
For five 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

 

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Angiotensin Converting Enzyme 2 (ACE-2). The suggested diluent is suitable for the analysis of most cell culture supernate, cell lysate, serum, plasma, and urine samples. Diluents for complex matrices, such astissue lysates, serum, plasma, and urine, should be evaluated prior to use in this DuoSet.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

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Human ACE-2 Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: ACE-2

Angiotensin converting enzyme 2 (ACE-2), also known as ACEH (ACE homolog), is an integral membrane protein and a zinc metalloprotease of the ACE family. The predicted human ACE-2 protein sequence consists of 805 amino acids, including a N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. ACE-2 cleaves angiotensins I and II as a carboxypeptidase. ACE-2 has been shown to be a functional receptor of the human coronaviruses SARS-CoV and SARS-CoV-2 . Amongst ongoing research into the cellular entry mechanism of coronavirus via ACE-2, an anti-human ACE-2 antibody (R&D Systems Catalog # AF933) has blocked this interaction.

Long Name:
Angiotensin I Converting Enzyme 2
Entrez Gene IDs:
59272 (Human); 70008 (Mouse); 302668 (Rat); 100144303 (Porcine); 480847 (Canine); 418623 (Chicken); 102130864 (Cynomolgus Monkey); 554349 (Feline); 101673097 (Ferret); 101823817 (Hamster); 108390919 (Malayan Pangolin)
Alternate Names:
ACE2; ACE-2; ACEH; ACEHangiotensin I converting enzyme 2; ACE-related carboxypeptidase; angiotensin I converting enzyme (peptidyl-dipeptidase A) 2; angiotensin-converting enzyme 2; Angiotensin-converting enzyme homolog; DKFZp434A014; EC 3.4.17; EC 3.4.17.23; Metalloprotease MPROT15

Citations for Human ACE-2 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Neutrophil Elastase decreases SARS-CoV-2 Spike protein binding to human bronchial epithelia by clipping ACE-2 ectodomian from the epithelial surface
    Authors: Kummarapurugu, AB;Hawkridge, AM;Ma, J;Osei, S;Martin, RK;Zheng, S;Voynow, JA;
    The Journal of biological chemistry
    Species: Human
    Sample Types: Plasma, Sputum Supernates
  2. Thraustochytrid hosts for expression of proteins relevant to SARS-CoV-2 intervention
    Authors: J Dahmen, A Vermeulen, S Payne, C Lippmeier
    PLoS ONE, 2023-04-12;18(4):e0283592.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Thraustochytrid hosts for expression of proteins relevant to SARS-CoV-2 intervention
    Authors: J Dahmen, A Vermeulen, S Payne, C Lippmeier
    PLoS ONE, 2023;18(4):e0283592.
    Species: Human
    Sample Types: Cell Culture Supernates
  4. Long Chain N3-PUFA Decreases ACE2 Protein Levels and Prevents SARS-CoV-2 Cell Entry
    Authors: S Huang, CG Taylor, P Zahradka
    International Journal of Molecular Sciences, 2022-11-10;23(22):.
    Species: Human
    Sample Types: Plasma
  5. Metalloprotease-Dependent S2'-Activation Promotes Cell-Cell Fusion and Syncytiation of SARS-CoV-2
    Authors: JV Harte, SL Wakerlin, AJ Lindsay, JV McCarthy, C Coleman-Va
    Viruses, 2022-09-21;14(10):.
    Species: Human
    Sample Types: Cell Culture Supernates
  6. Endothelial dysfunction and altered endothelial biomarkers in patients with post-COVID-19 syndrome and chronic fatigue syndrome (ME/CFS)
    Authors: M Haffke, H Freitag, G Rudolf, M Seifert, W Doehner, N Scherbakov, L Hanitsch, K Wittke, S Bauer, F Konietschk, F Paul, J Bellmann-S, C Kedor, C Scheibenbo, F Sotzny
    Journal of Translational Medicine, 2022-03-22;20(1):138.
    Species: Human
    Sample Types: Serum
  7. Oxidative stress-induced endothelial dysfunction and decreased vascular nitric oxide in COVID-19 patients
    Authors: V Montiel, I Lobysheva, L Gérard, M Vermeersch, D Perez-Morg, T Castelein, JB Mesland, P Hantson, C Collienne, D Gruson, MA van Dievoe, A Persu, C Beauloye, M Dechamps, L Belkhir, A Robert, M Derive, PF Laterre, AHJ Danser, X Wittebole, JL Balligand
    EBioMedicine, 2022-02-23;77(0):103893.
    Species: Human
    Sample Types: Serum
  8. Whole Exome Sequencing Identifies a Rare Mutation in NACAD as a Possible Cause of COVID Orchitis in Brothers
    Authors: R Reddy, I Efimenko, W Chertman, T Kohn, P Diaz, D Seetharam, K Khodamorad, E Kresch, R Ramasamy
    Urology, 2021-10-20;0(0):.
    Species: Human
    Sample Types: Plasma
  9. Clinical grade ACE2 as a universal agent to block SARS-CoV-2 variants
    Authors: G Wirnsberge, V Monteil, B Eaton, E Postnikova, M Murphy, B Braunsfeld, I Crozier, F Kricek, J Niederhöfe, A Schwarzböc, H Breid, A Sanchez Ji, A Bugajska-S, A Dohnal, C Ruf, R Gugenberge, A Hagelkruys, N Montserrat, MR Holbrook, C Oostenbrin, RH Shoemaker, A Mirazimi, JM Penninger
    bioRxiv : the preprint server for biology, 2021-09-10;0(0):.
    Species: Human
    Sample Types: Virus
    Applications: ELISA Capture
  10. Circulating Soluble ACE2 and Upstream microRNA Expressions in Serum of Type 2 Diabetes Mellitus Patients
    Authors: NM Elemam, H Hasswan, H Aljaibeji, N Sulaiman
    International Journal of Molecular Sciences, 2021-05-17;22(10):.
    Species: Human
    Sample Types: Serum
  11. Hypoxia induces expression of angiotensin-converting enzyme II in alveolar epithelial cells: Implications for the pathogenesis of acute lung injury in COVID-19
    Authors: A Sturrock, E Zimmerman, M Helms, TG Liou, R Paine
    Physiological Reports, 2021-05-01;9(9):e14854.
    Species: Human
    Sample Types: Cell Lysates

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