Does the Human Active MMP-9 Fluorokine E Kit detect active or pro-MMP-9?

Name: Human Active MMP-9 Fluorokine E Kit
Brand: R&D Systems
SKU: F9M00
Rating: 5 (2 reviews)

Without using APMA before substrate addition, the kit measures the levels of endogenous active MMP-9. With using APMA before substrate addition, the kit measures the levels of endogenous active MMP-9 and levels of the MMP-9 that can be activated by APMA, which reflects the sum of active MMP-9 and pro-MMP-9.

Human Active MMP-9 Fluorokine E Kit

Catalog #: F9M00
20 Citations 2 Reviews Datasheet / COA / SDS

Contains Mercury, Dispose According to Local, State or Federal Laws

Catalog #	Availability	Size / Price	Qty
F9M00

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Product Details

Citations (20)

FAQs

Reviews (2)

Summary Product Datasheets Background

Human Active MMP-9 Fluorokine E Kit Summary

Specifications

Source

N/A

Shipping Conditions

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Species

Human

Product Datasheets

Product Datasheet

COA

SDS

Background: MMP-9

The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).

Long Name

Matrix Metalloproteinase 9

Entrez Gene IDs

4318 (Human); 17395 (Mouse); 81687 (Rat); 102117693 (Cynomolgus Monkey)

Alternate Names

92 kDa gelatinase; 92 kDa type IV collagenase; CLG4B; EC 3.4.24; EC 3.4.24.35; Gelatinase B; GELB; macrophage gelatinase; MANDP2; matrix metallopeptidase 9; matrix metalloproteinase 9; matrix metalloproteinase-9; MMP9; MMP-9; type V collagenase

Related Research Areas

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov.

Citations for Human Active MMP-9 Fluorokine E Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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The combination of propylene glycol and vegetable glycerin e-cigarette aerosols induces airway inflammation and mucus hyperconcentration
Authors: Kim, MD;Chung, S;Baumlin, N;Qian, J;Montgomery, RN;Sabater, J;Berkland, C;Salathe, M;
Scientific reports 2024-01-23
C9orf72-ALS human iPSC microglia are pro-inflammatory and toxic to co-cultured motor neurons via MMP9
Authors: Vahsen, BF;Nalluru, S;Morgan, GR;Farrimond, L;Carroll, E;Xu, Y;Cramb, KML;Amein, B;Scaber, J;Katsikoudi, A;Candalija, A;Carcolé, M;Dafinca, R;Isaacs, AM;Wade-Martins, R;Gray, E;Turner, MR;Cowley, SA;Talbot, K;
Nature communications 2023-09-22
Enhancement of Farnesoid X Receptor Inhibits Migration, Adhesion and Angiogenesis through Proteasome Degradation and VEGF Reduction in Bladder Cancers
Authors: CR Lai, HH Wang, HH Chang, YL Tsai, WC Tsai, CR Lee, CY Changchien, YC Cheng, ST Wu, Y Chen
International Journal of Molecular Sciences, 2022-05-09;23(9):. 2022-05-09
The psychoactive drug of abuse mephedrone differentially disrupts blood-brain barrier properties
Authors: TP Buzhdygan, CR Rodrigues, HM McGary, JA Khan, AM Andrews, SM Rawls, SH Ramirez
Journal of Neuroinflammation, 2021-03-01;18(1):63. 2021-03-01
Enhanced closed eye neutrophil degranulation in dry eye disease
Authors: CK Postnikoff, K Held, V Viswanath, KK Nichols
Ocul Surf, 2020-09-02;18(4):841-851. 2020-09-02
Cyclooxygenase-2 in tumor-associated macrophages promotes metastatic potential of breast cancer cells through Akt pathway
Int. J. Biol. Sci, 2016-11-26;12(12):1533-1543. 2016-11-26
The differential effects of azithromycin on the airway epithelium inï¿½vitro and inï¿½vivo
Physiol Rep, 2016-09-01;4(18):. 2016-09-01
Interplay between Matrix Metalloproteinase-9, Matrix Metalloproteinase-2, and Interleukins in Multiple Sclerosis Patients
Dis Markers, 2016-07-31;2016(0):3672353. 2016-07-31
The TNF Family Molecules LIGHT and Lymphotoxin alphabeta Induce a Distinct Steroid-Resistant Inflammatory Phenotype in Human Lung Epithelial Cells.
Authors: da Silva Antunes R, Madge L, Soroosh P, Tocker J, Croft M
J Immunol, 2015-07-24;195(5):2429-41. 2015-07-24
Regulation of MMP-9 by a WIN-binding site in the monocyte-macrophage system independent from cannabinoid receptors.
Authors: Tauber, Svantje, Paulsen, Katrin, Wolf, Susanne, Synwoldt, Peggy, Pahl, Andreas, Schneider-Stock, Regine, Ullrich, Oliver
PLoS ONE, 2012-11-06;7(11):e48272. 2012-11-06

Host gene-encoded severe lung TB: from genes to the potential pathways.
Authors: Ganachari, M, Guio, H, Zhao, N, Flores-Villanueva, P O
Genes Immun, 2012-09-20;13(8):605-20. 2012-09-20

Early Elevation of Matrix Metalloproteinase-8 and -9 in Pediatric ARDS Is Associated with an Increased Risk of Prolonged Mechanical Ventilation.
Authors: Kong MY, Li Y, Oster R, Gaggar A, Clancy JP
PLoS ONE, 2011-08-03;6(8):e22596. 2011-08-03

Primary human acute myelogenous leukemia cells release matrix metalloproteases and their inhibitors: release profile and pharmacological modulation.
Authors: Reikvam H, Hatfield KJ, Oyan AM, Kalland KH, Kittang AO, Bruserud O
Eur. J. Haematol., 2009-11-17;84(3):239-51. 2009-11-17

Serum matrix metalloproteinases in adult CF patients: Relation to pulmonary exacerbation.
Authors: Roderfeld M, Rath T, Schulz R, Seeger W, Tschuschner A, Graf J, Roeb E
J. Cyst. Fibros., 2009-07-14;8(5):338-47. 2009-07-14

Salbutamol up-regulates matrix metalloproteinase-9 in the alveolar space in the acute respiratory distress syndrome.
Authors: O'Kane CM, McKeown SW, Perkins GD, Bassford CR, Gao F, Thickett DR, McAuley DF
Crit. Care Med., 2009-07-01;37(7):2242-9. 2009-07-01

The presence of a matrix-derived neutrophil chemoattractant in bronchiolitis obliterans syndrome after lung transplantation.
Authors: Hardison MT, Galin FS, Calderon CE, Djekic UV, Parker SB, Wille KM, Jackson PL, Oster RA, Young KR, Blalock JE, Gaggar A
J. Immunol., 2009-04-01;182(7):4423-31. 2009-04-01

Matrix metalloproteinase activity in pediatric acute lung injury.
Authors: Kong MY, Gaggar A, Li Y
Int J Med Sci, 2008-12-16;6(1):9-17. 2008-12-16

Differential proteolytic enzyme activity in eosinophilic and neutrophilic asthma.
Authors: Simpson JL, Scott RJ, Boyle MJ, Gibson PG
Am. J. Respir. Crit. Care Med., 2005-05-18;172(5):559-65. 2005-05-18

Comparative effects of AT1-antagonism and angiotensin-converting enzyme inhibition on markers of inflammation and platelet aggregation in patients with coronary artery disease.
Authors: Schieffer B, Bunte C, Witte J, Hoeper K, Boger RH, Schwedhelm E, Drexler H
J. Am. Coll. Cardiol., 2004-07-21;44(2):362-8. 2004-07-21

Implications for matrix metalloproteinases as modulators of pediatric lung disease.
Authors: Winkler MK, Foldes JK, Bunn RC, Fowlkes JL
Am. J. Physiol. Lung Cell Mol. Physiol., 2002-11-27;284(4):L557-65. 2002-11-27

FAQs

Does the Human Active MMP-9 Fluorokine E Kit detect active or pro-MMP-9?
- Without using APMA before substrate addition, the kit measures the levels of endogenous active MMP-9. With using APMA before substrate addition, the kit measures the levels of endogenous active MMP-9 and levels of the MMP-9 that can be activated by APMA, which reflects the sum of active MMP-9 and pro-MMP-9.

View all Activity Assay FAQs