Human AG-3 Antibody

Catalog #: MAB6307 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6307
MAB6307-SP

Detection of Human AG‑3 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human AG-3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human AG-3 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human AG-2 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 774855

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human AG-3
Ile22-Leu166
Accession # Q8TD06

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human AG‑3 by Western Blot. Western blot shows lysates of T47D human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human AG-3 Monoclonal Antibody (Catalog # MAB6307) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for AG-3 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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AG‑3 in Human Breast. AG-3 was detected in immersion fixed paraffin-embedded sections of human breast using Mouse Anti-Human AG-3 Monoclonal Antibody (Catalog # MAB6307) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to cytoplasm of glandular cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: AG-3

AG-3 (Anterior gradient protein 3; also known as AGR3 and BCMP11) is a 17 kDa (predicted) member of the AGR family of proteins. It is expressed by intestinal epithelial and breast cancer cells. AG-3 is presumably secreted and binds to both alpha -dystroglycan and C4.4a/LYPD3. Mature human AG-3 is 145 amino acids (aa) in length (aa 22‑166). Over aa 22-166, human AG-3 shares 92% amino acid sequence identity with mouse AG-3.

Long Name

Anterior Gradient 3 Homolog

Entrez Gene IDs

155465 (Human); 403205 (Mouse); 298959 (Rat)

Alternate Names

AG3; AG-3; AGR3; anterior gradient homolog 3 (Xenopus laevis); BCMP11; BCMP11AG3; Breast cancer membrane protein 11; hAG-3member 18; PDIA18