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Human alpha-Fetoprotein/AFP Antibody

Catalog #: MAB1369
21 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1369
MAB1369-SP
alpha ‑Fetoprotein/AFP in BG01V Human Embryonic Stem Cells.
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Citations (21)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human alpha-Fetoprotein/AFP Antibody Summary

Species Reactivity
Human
Specificity
Detects human alpha -Fetoprotein in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 189506
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human umbilical cord serum-derived alpha -Fetoprotein
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
CyTOF-reported
This clone has been commercially reported for use in CyTOF®. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below
Knockout Validated
alpha -Fetoprotein/AFP was specifically detected in immersion fixed HepG2 human hepatocellular carcinoma cell line but is not detected in alpha -Fetoprotein/AFP knockout (KO) HepG2 cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry a-Fetoprotein/AFP antibody in BG01V Human Embryonic Stem Cells by Immunocytochemistry (ICC). View Larger

alpha ‑Fetoprotein/AFP in BG01V Human Embryonic Stem Cells. a-Fetoprotein/AFP was detected in immersion fixed BG01V human embryonic stem cells differentiated to hepatocytes using Mouse Anti-Human a-Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB1369) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Intracellular Staining by Flow Cytometry Detection of a-Fetoprotein/AFP antibody in HepG2 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of alpha ‑Fetoprotein/AFP in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human a-Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB1369, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Knockout Validated a-Fetoprotein/AFP Antibody Specificity is Shown by Immunocytochemistry antibody in Knockout Cell Line. View Larger

alpha ‑Fetoprotein/AFP Specificity is Shown by Immunocytochemistry in Knockout Cell Line. a-Fetoprotein/AFP was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line but is not detected in a-Fetoprotein/AFP knockout (KO) HepG2 Human Cell Line cell line using Mouse Anti-Human a-Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB1369) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha-Fetoprotein/AFP

AFP ( alpha -Fetoprotein) is a 69-73 kDa member of the ALB/AFP/VDB family of proteins. alpha -Fetoprotein is a major plasma protein in the fetus. Its concentration is normally low in the adult except when produced by certain tumors. It is secreted by fetal liver and serves as a carrier molecule for phytoestrogens, heavy metals (Cu and Ni), estrogen and fatty acids. Mature human AFP is 591 amino acids (aa) in length. It contains three albumin domains (aa 19-210, 211-402 and 403-601), plus 15 intrachain disulfide bonds. Mature human AFP shares approximately 66% aa identity with mouse AFP.

References
  1. Matsumura, M. et al. (2001) Hepatol. Res. 20:84
  2. Deutsch, H.F. et al. (2000) Tumor Biol. 21:267
    Entrez Gene IDs
    174 (Human); 11576 (Mouse); 24177 (Rat)
    Alternate Names
    AFP; Alpha-1-fetoprotein; alpha-fetoglobulin; alpha-Fetoprotein; DSCAM2; FETA; HP; HPAFP

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    Citations for Human alpha-Fetoprotein/AFP Antibody

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    21 Citations: Showing 1 - 10
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    1. Derivation of induced pluripotent stem cells in Japanese macaque (Macaca fuscata)
      Authors: R Nakai, M Ohnuki, K Kuroki, H Ito, H Hirai, R Kitajima, T Fujimoto, M Nakagawa, W Enard, M Imamura
      Sci Rep, 2018-08-15;8(1):12187.
    2. Detailed Characterization of Human Induced Pluripotent Stem Cells Manufactured for Therapeutic Applications
      Authors: Behnam Ahmadian Baghbaderani, Adhikarla Syama, Renuka Sivapatham, Ying Pei, Odity Mukherjee, Thomas Fellner et al.
      Stem Cell Reviews and Reports
    3. Miniaturized 3D bone marrow tissue model to assess response to Thrombopoietin-receptor agonists in patients
      Authors: Christian A Di Buduo, Pierre-Alexandre Laurent, Carlo Zaninetti, Larissa Lordier, Paolo M Soprano, Aikaterini Ntai et al.
      eLife
    4. 3D-cultured blastoids model human embryogenesis from pre-implantation to early gastrulation stages
      Authors: Karvas, RM;Zemke, JE;Ali, SS;Upton, E;Sane, E;Fischer, LA;Dong, C;Park, KM;Wang, F;Park, K;Hao, S;Chew, B;Meyer, B;Zhou, C;Dietmann, S;Theunissen, TW;
      Cell stem cell
      Species: Human
      Sample Types: Protein
      Applications: Immunocytochemistry
    5. Establishment of a human induced pluripotent stem cell line (TAUi008-A) derived from a multiple sclerosis patient
      Authors: J Lotila, T Hyvärinen, H Skottman, L Airas, S Narkilahti, S Hagman
      Oncogene, 2022-07-11;63(0):102865.
      Species: Human
      Sample Types: Whole Cells
      Applications: Flow Cytometry, ICC
    6. Generation and characterization of IMAGINi013-A, an induced pluripotent stem cell line generated from a healthy donor
      Authors: C Banal, N Lefort
      Stem Cell Research, 2022-03-21;61(0):102755.
      Species: Human
      Sample Types: Whole Cells
      Applications: ICC
    7. Establishment of an integration-free human induced pluripotent stem cell line (TJCi001-A) from normal bone marrow-derived mesenchymal stem cells
      Authors: NY Choi, Y Lee, YS Park, K Ko, B Park, YG Koh
      Stem Cell Research, 2021-08-03;55(0):102484.
      Species: Human
      Sample Types: Whole Cells
      Applications: ICC
    8. Generation of an iPSC line (IMAGINi022-A) from a patient carrying a SOX10 missense mutation and presenting with deafness, depigmentation and progressive neurological impairment
      Authors: C Banal, E Quelennec, W Bertani-To, N Gacem, J Amiel, S Marlin, F Petit, V Pingault, N Lefort, N Bondurand
      Stem Cell Res, 2020-08-02;48(0):101936.
      Species: Human
      Sample Types: Whole Cells
      Applications: ICC
    9. Generation of an induced pluripotent stem cell (iPSC) line (IMAGINi007) from a patient with steroid-resistant nephrotic syndrome carrying the homozygous p.R138Q mutation in the podocin-encoding NPHS2 gene
      Authors: G Menara, N Lefort, C Antignac, G Mollet
      Stem Cell Res, 2020-06-18;46(0):101878.
      Species: Human
      Sample Types: Whole Cells
      Applications: ICC
    10. Modeling of early neural development in vitro by direct neurosphere formation culture of chimpanzee induced pluripotent stem cells
      Authors: R Kitajima, R Nakai, T Imamura, T Kameda, D Kozuka, H Hirai, H Ito, H Imai, M Imamura
      Stem Cell Res, 2020-02-28;44(0):101749.
      Species: Pan troglodytes (Chimpanzee)
      Sample Types: Whole Cells
      Applications: ICC
    11. Small non-coding RNA landscape of extracellular vesicles from human stem cells
      Authors: S Kaur, AG Abu-Shahba, RO Paananen, H Hongisto, H Hiidenmaa, H Skottman, R Seppänen-K, B Mannerströ
      Sci Rep, 2018-10-19;8(1):15503.
      Species: Human
      Sample Types: Whole Cells
      Applications: Differentiation, Differentiation
    12. Modeling the neuropsychiatric manifestations of Lowe syndrome using induced pluripotent stem cells: defective F-actin polymerization and WAVE-1 expression in neuronal cells
      Authors: J Barnes, F Salas, R Mokhtari, H Dolstra, E Pedrosa, HM Lachman
      Mol Autism, 2018-08-15;9(0):44.
      Species: Human
      Sample Types: Organoids
      Applications: IHC
    13. Human-Induced Pluripotent Stem Cells Manufactured Using a Current Good Manufacturing Practice-Compliant Process Differentiate Into Clinically Relevant Cells From Three Germ Layers
      Authors: M Shafa, F Yang, T Fellner, MS Rao, BA Baghbadera
      Front Med (Lausanne), 2018-03-15;5(0):69.
      Species: Human
      Sample Types: Whole Cells
      Applications: ICC
    14. Bulk cell density and Wnt/TGFbeta signalling regulate mesendodermal patterning of human pluripotent stem cells
      Nat Commun, 2016-12-09;7(0):13602.
      Species: Human
      Sample Types: Cell Culture Supernates
      Applications: Western Blot
    15. A Newly Defined and Xeno-Free Culture Medium Supports Every-Other-Day Medium Replacement in the Generation and Long-Term Cultivation of Human Pluripotent Stem Cells
      PLoS ONE, 2016-09-08;11(9):e0161229.
      Species: Human
      Sample Types: Whole Cells
      Applications: IHC
    16. Frontotemporal dementia-associated N279K tau mutant disrupts subcellular vesicle trafficking and induces cellular stress in iPSC-derived neural stem cells.
      Authors: Wren M, Zhao J, Liu C, Murray M, Atagi Y, Davis M, Fu Y, Okano H, Ogaki K, Strongosky A, Tacik P, Rademakers R, Ross O, Dickson D, Wszolek Z, Kanekiyo T, Bu G
      Mol Neurodegener, 2015-09-15;10(0):46.
      Species: Human
      Sample Types: Whole Cells
      Applications: IHC
    17. A stable chimeric fibroblast growth factor (FGF) can successfully replace basic FGF in human pluripotent stem cell culture.
      Authors: Onuma, Yasuko, Higuchi, Kumiko, Aiki, Yasuhiko, Shu, Yujing, Asada, Masahiro, Asashima, Makoto, Suzuki, Masashi, Imamura, Toru, Ito, Yuzuru
      PLoS ONE, 2015-04-07;10(4):e0118931.
      Species: Human
      Sample Types: Whole Cells
      Applications: ICC
    18. Embryoid body formation of human amniotic fluid stem cells depends on mTOR.
      Authors: Valli A, Rosner M, Fuchs C, Siegel N, Bishop CE, Dolznig H, Madel U, Feichtinger W, Atala A, Hengstschlager M
      Oncogene, 2009-11-23;29(7):966-77.
      Species: Human
      Sample Types: Whole Cells
      Applications: ICC
    19. Nodal inhibits differentiation of human embryonic stem cells along the neuroectodermal default pathway.
      Authors: Vallier L, Reynolds D, Pedersen RA
      Dev. Biol., 2004-11-15;275(2):403-21.
      Species: Human
      Sample Types: Whole Cells
      Applications: ICC
    20. Central role of complement membrane attack complex in monosodium urate crystal-induced neutrophilic rabbit knee synovitis.
      Authors: Tramontini N, Huber C, Liu-Bryan R, Terkeltaub RA, Kilgore KS
      Arthritis Rheum., 2004-08-01;50(8):2633-9.
      Species: Human
      Sample Types: Whole Cells
      Applications: ICC
    21. Long-Term Stability and Differentiation Potential of Cryopreserved cGMP-Compliant Human Induced Pluripotent Stem Cells
      Authors: Mehdi Shafa, Tylor Walsh, Krishna Morgan Panchalingam, Thomas Richardson, Laura Menendez, Xinghui Tian et al.
      International Journal of Molecular Sciences

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