Human alpha-Methylacyl-CoA Racemase/AMACR Antibody

Catalog #: AF7508
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7508
AF7508-SP

Detection of Human alpha ‑Methylacyl‑CoA Racemase/AMACR by Western Blot.

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All alpha-Methylacyl-CoA Racemase/AMACR Antibodies

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human alpha-Methylacyl-CoA Racemase/AMACR Antibody Summary

Species Reactivity

Human

Specificity

Detects human alpha ‑Methylacyl‑CoA Racemase/AMACR in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human alpha ‑Methylacyl‑CoA Racemase/AMACR
Met1-Leu382
Accession # Q9UHK6

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.2 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human alpha ‑Methylacyl‑CoA Racemase/AMACR by Western Blot. Western blot shows lysates of human kidney tissue, human liver tissue, and human prostate tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human a-Methylacyl-CoA Racemase/AMACR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7508) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for a-Methylacyl-CoA Racemase/AMACR at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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alpha ‑Methylacyl‑CoA Racemase/AMACR in Human Prostate Gland. a-Methylacyl-CoA Racemase/AMACR was detected in immersion fixed paraffin-embedded sections of human prostate gland using Sheep Anti-Human a-Methylacyl-CoA Racemase/AMACR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7508) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha-Methylacyl-CoA Racemase/AMACR

AMACR (Alpha-MethylAcyl-CoA Racemase; also 2-methylacyl racemase) is a 43-46 kDa member of the CaiB/BaiF CoA-transferase family of enzymes. It is widely expressed, being found in fibroblasts, hepatocytes, plus tumorigenic prostatic and colonic epithelium. Within these cells, it is localized to peroxisomes (organelles that participate in the breakdown fatty acids into 2-carbon blocks) and occasionally mitochondria, and appears to racemize 2-methyl-branched fatty acids. This ability is necessary for the degradation of branched fatty acids such as C19 dietary pristanic acid. Pristanic acid occurs in both an S- and R-methylated stereoisomer, but can only be initially degraded in the S- isomeric form. AMACR converts the R- to the S-isoform, initiating fatty acid processing. Human AMACR(-IA) is 382 amino acids (aa) in length. It contains an N-terminal mitochondrial targeting sequence (aa 22-85) that overlaps the enzymatic region (aa 53-231), and a C-terminal peroxisomal targeting motif (aa 379-382). There are multiple potential splice variants. Over aa 132-382, there are three aa substitutions, one that is 66 aa in length (AMACR‑IB), a second that is 147 aa in length (AMACR-IIB), and a third that is 98 aa in length. Over aa 249-382, there are two aa substitutions, one that is 13 aa in length (AMACR‑IIAs), and another that is 41 aa in length (AMACR-IIA). There is also a sixth potential splice variant that shows a 16 aa substitution for aa 378-382. Full-length human AMACR(-IA) shares 77% aa sequence identity with mouse AMACR.

Entrez Gene IDs

23600 (Human); 17117 (Mouse); 25284 (Rat)

Alternate Names

2-methylacyl-CoA racemase; alpha-Methylacyl-CoA Racemase; alphaMethylacylCoARacemase; AMACR; AMACRD; CBAS4; EC 5.1.99.4; RACE; RM

Product Datasheets

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Citation for Human alpha-Methylacyl-CoA Racemase/AMACR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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