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Human alpha-Smooth Muscle Actin APC-conjugated Antibody

Catalog #: IC1420A
14 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
IC1420A
Detection of alpha ‑Smooth Muscle Actin in Human Blood Monocytes by Flow Cytometry.
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Citations (14)
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Human alpha-Smooth Muscle Actin APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human  alpha -Smooth Muscle Actin.
Source
Monoclonal Mouse IgG2A Clone # 1A4
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
alpha -Smooth Muscle Actin N-terminal Synthetic Decapeptide
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of a-Smooth Muscle Actin antibody in Human Blood Monocytes antibody by Flow Cytometry. View Larger

Detection of alpha ‑Smooth Muscle Actin in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human a-Smooth Muscle Actin APC-conjugated Monoclonal Antibody (Catalog # IC1420A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: alpha-Smooth Muscle Actin

alpha ‑Smooth Muscle Actin has been frequently used as a marker of smooth muscle differentiation (1, 2).

References
  1. Skalli, O. et al. (1986) J. Cell Biol. 103:2787.
  2. Oishi, K. et al. (2002) J. Physiol. 540:139.
Long Name
Actin, Alpha 2, Smooth Muscle, Aorta
Entrez Gene IDs
59 (Human); 11475 (Mouse); 81633 (Rat)
Alternate Names
AAT6; ACTA2; Actin alpha 2; actin, alpha 2, smooth muscle, aorta; actin, aortic smooth muscle; ACTSA; ACTVS; alpha 2 actin; alpha SMA; alpha smooth muscle actin; alpha-actin-2; alpha-SMA; alphaSmooth Muscle Actin; alpha-Smooth Muscle Actin; a-sma; Cell growth-inhibiting gene 46 protein; growth-inhibiting gene 46; MYMY5; SMA; smooth muscle actin

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Citations for Human alpha-Smooth Muscle Actin APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. A human multi-cellular model shows how platelets drive production of diseased extracellular matrix and tissue invasion
    Authors: Malacrida B, Nichols S, Maniati E et al.
    iScience
  2. Inhibition of the mTOR pathway in abdominal aortic aneurysm: implications of smooth muscle cell contractile phenotype, inflammation, and aneurysm expansion
    Authors: Guangxin Li, Lingfeng Qin, Lei Wang, Xuan Li, Alexander W. Caulk, Jian Zhang et al.
    American Journal of Physiology-Heart and Circulatory Physiology
  3. Modelling TGF beta R and Hh pathway regulation of prognostic matrisome molecules in ovarian cancer
    Authors: Delaine-Smith R, Maniati E, Malacrida B et al.
    iScience
  4. Mesothelial cells with mesenchymal features enhance peritoneal dissemination by forming a protumorigenic microenvironment
    Authors: Yonemura, A;Semba, T;Zhang, J;Fan, Y;Yasuda-Yoshihara, N;Wang, H;Uchihara, T;Yasuda, T;Nishimura, A;Fu, L;Hu, X;Wei, F;Kitamura, F;Akiyama, T;Yamashita, K;Eto, K;Iwagami, S;Iwatsuki, M;Miyamoto, Y;Matsusaki, K;Yamasaki, J;Nagano, O;Saya, H;Song, S;Tan, P;Baba, H;Ajani, JA;Ishimoto, T;
    Cell reports
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Orthotopic model of pancreatic cancer using CD34+ humanized mice and generation of tumor organoids from humanized tumors
    Authors: Hye Jeong, J;Park, S;Lee, S;Kim, Y;Kyong Shim, I;Jeong, SY;Kyung Choi, E;Kim, J;Jun, E;
    International immunopharmacology
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Targeting Periostin Expression Makes Pancreatic Cancer Spheroids More Vulnerable to Natural Killer Cells
    Authors: D Karakas, M Erkisa, RO Akar, G Akman, EY Senol, E Ulukaya
    Biomedicines, 2023-01-19;11(2):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. microRNA expression profile in Smooth Muscle Cells isolated from thoracic aortic aneurysm samples
    Authors: A Kasprzyk-P, A Wojciechow, M Kuc, J Zielinski, A Parulski, M Kusmierczy, A Lutynska, K Kozar-Kami
    Adv Med Sci, 2019-04-22;64(2):331-337.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Adipose-tissue-derived therapeutic cells in their natural environment as an autologous cell therapy strategy: the microtissue-stromal vascular fraction
    Authors: S Nürnberger, C Lindner, J Maier, K Strohmeier, C Wurzer, P Slezak, S Suessner, W Holnthoner, H Redl, S Wolbank, E Priglinger
    Eur Cell Mater, 2019-02-22;37(0):113-133.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Transcriptome network analysis identifies protective role of the LXR/SREBP-1c axis in murine pulmonary fibrosis
    Authors: S Shichino, S Ueha, S Hashimoto, M Otsuji, J Abe, T Tsukui, S Deshimaru, T Nakajima, M Kosugi-Kan, FH Shand, Y Inagaki, H Shimano, K Matsushima
    JCI Insight, 2019-01-10;4(1):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Flow Cytometry
  10. Lung fibroblasts express a miR-19a-19b-20a sub-cluster to suppress TGF-?-associated fibroblast activation in murine pulmonary fibrosis
    Authors: K Souma, S Shichino, S Hashimoto, S Ueha, T Tsukui, T Nakajima, HI Suzuki, FHW Shand, Y Inagaki, T Nagase, K Matsushima
    Sci Rep, 2018-11-09;8(1):16642.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Mutational analysis of AKT1 and PIK3CA in intraductal papillomas of the breast with special reference to cellular components
    Authors: C Mishima, N Kagara, JI Ikeda, E Morii, T Miyake, T Tanei, Y Naoi, M Shimoda, K Shimazu, SJ Kim, S Noguchi
    Am. J. Pathol., 2018-02-16;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Fibroblast Heterogeneity and Immunosuppressive Environment in Human Breast Cancer
    Authors: A Costa, Y Kieffer, A Scholer-Da, F Pelon, B Bourachot, M Cardon, P Sirven, I Magagna, L Fuhrmann, C Bernard, C Bonneau, M Kondratova, I Kuperstein, A Zinovyev, AM Givel, MC Parrini, V Soumelis, A Vincent-Sa, F Mechta-Gri
    Cancer Cell, 2018-02-15;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: FACS
  13. High-throughput immunophenotypic characterization of bone marrow- and cord blood-derived mesenchymal stromal cells reveals common and differentially expressed markers: identification of angiotensin-converting enzyme (CD143) as a marker differentially expressed between adult and perinatal tissue sources
    Authors: E Amati, O Perbellini, G Rotta, M Bernardi, K Chieregato, S Sella, F Rodeghiero, M Ruggeri, G Astori
    Stem Cell Res Ther, 2018-01-16;9(1):10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Endothelial-to-mesenchymal transition drives atherosclerosis progression.
    Authors: Chen PY, Qin L, Baeyens N et al.
    J Clin Invest

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