Human alpha-Smooth Muscle Actin APC-conjugated Antibody
Human alpha-Smooth Muscle Actin APC-conjugated Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
![Detection of a-Smooth Muscle Actin antibody in Human Blood Monocytes antibody by Flow Cytometry. Detection of a-Smooth Muscle Actin antibody in Human Blood Monocytes antibody by Flow Cytometry.](https://resources.rndsystems.com/images/datasheets/antibody/alphaSmooth_Muscle_Actin_IC1420A_Flow_Cytometry_13218.jpg)
Detection of alpha ‑Smooth Muscle Actin in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human a-Smooth Muscle Actin APC-conjugated Monoclonal Antibody (Catalog # IC1420A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
![Detection of Mouse alpha-Smooth Muscle Actin by Immunohistochemistry Detection of Mouse alpha-Smooth Muscle Actin by Immunohistochemistry](https://resources.rndsystems.com/images/datasheets/ic1420a_human-alpha-smooth-muscle-actin-allophycocyanin-mab-cl-1a4-8120255531695.jpg)
Detection of Mouse alpha-Smooth Muscle Actin by Immunohistochemistry FGFR1 and TGF beta signaling activity in smooth muscle cells in a mouse atherosclerosis modelADissected mouse aorta demonstrating lipid‐rich plaques in brachiocephalic artery after 4 months of high‐fat diet compared to the normal diet in Apoe−/− mice. Panels b, d are cross sections of brachiocephalic artery from a, c stained with Oil Red O. L: lumen. Scale bar: 4 mm. n = 3 mice per group.BHistological analysis of mouse normal artery or atherosclerotic plaque in brachiocephalic artery with anti‐SM alpha ‐actin, anti‐Notch3, and anti‐SM‐MHC antibodies. Nuclei were counterstained with DAPI (blue). L: lumen. Scale bar: 62 μm. n = 3 mice per group.C–FAnalysis of brachiocephalic artery of Apoe−/− mice maintained for 4 months on either normal or high‐fat diet using anti‐CD31 (green), anti‐p‐FGFR1 (red; C), anti‐FGFR1 (red; D), anti‐p‐Smad2 (red; E), and anti‐p‐Smad3 (red; F) antibodies. Nuclei were counterstained with DAPI (blue). Scale bar: 62 μm. L: lumen. M: media. n = 6 mice per group.G–JQuantification of the number of medial smooth muscle cells expressing p‐FGFR1, FGFR1, p‐Smad2, and p‐Smad3. ND: normal diet. HFD: high‐fat diet.Data information: All data shown as means ± SD (***P < 0.001 compared to ND, NS: not significant compared to ND; unpaired two‐tailed Student's t‐test). A full table of P‐values for this figure is shown in Appendix Table S1. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/27189169), licensed under a CC-BY license. Not internally tested by R&D Systems.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: alpha-Smooth Muscle Actin
alpha ‑Smooth Muscle Actin has been frequently used as a marker of smooth muscle differentiation (1, 2).
- Skalli, O. et al. (1986) J. Cell Biol. 103:2787.
- Oishi, K. et al. (2002) J. Physiol. 540:139.
Product Datasheets
Citations for Human alpha-Smooth Muscle Actin APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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A human multi-cellular model shows how platelets drive production of diseased extracellular matrix and tissue invasion
Authors: Malacrida B, Nichols S, Maniati E et al.
iScience
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Inhibition of the mTOR pathway in abdominal aortic aneurysm: implications of smooth muscle cell contractile phenotype, inflammation, and aneurysm expansion
Authors: Guangxin Li, Lingfeng Qin, Lei Wang, Xuan Li, Alexander W. Caulk, Jian Zhang et al.
American Journal of Physiology-Heart and Circulatory Physiology
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Modelling TGF beta R and Hh pathway regulation of prognostic matrisome molecules in ovarian cancer
Authors: Delaine-Smith R, Maniati E, Malacrida B et al.
iScience
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Mesothelial cells with mesenchymal features enhance peritoneal dissemination by forming a protumorigenic microenvironment
Authors: Yonemura, A;Semba, T;Zhang, J;Fan, Y;Yasuda-Yoshihara, N;Wang, H;Uchihara, T;Yasuda, T;Nishimura, A;Fu, L;Hu, X;Wei, F;Kitamura, F;Akiyama, T;Yamashita, K;Eto, K;Iwagami, S;Iwatsuki, M;Miyamoto, Y;Matsusaki, K;Yamasaki, J;Nagano, O;Saya, H;Song, S;Tan, P;Baba, H;Ajani, JA;Ishimoto, T;
Cell reports
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Orthotopic model of pancreatic cancer using CD34+ humanized mice and generation of tumor organoids from humanized tumors
Authors: Hye Jeong, J;Park, S;Lee, S;Kim, Y;Kyong Shim, I;Jeong, SY;Kyung Choi, E;Kim, J;Jun, E;
International immunopharmacology
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Targeting Periostin Expression Makes Pancreatic Cancer Spheroids More Vulnerable to Natural Killer Cells
Authors: D Karakas, M Erkisa, RO Akar, G Akman, EY Senol, E Ulukaya
Biomedicines, 2023-01-19;11(2):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
microRNA expression profile in Smooth Muscle Cells isolated from thoracic aortic aneurysm samples
Authors: A Kasprzyk-P, A Wojciechow, M Kuc, J Zielinski, A Parulski, M Kusmierczy, A Lutynska, K Kozar-Kami
Adv Med Sci, 2019-04-22;64(2):331-337.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Adipose-tissue-derived therapeutic cells in their natural environment as an autologous cell therapy strategy: the microtissue-stromal vascular fraction
Authors: S Nürnberger, C Lindner, J Maier, K Strohmeier, C Wurzer, P Slezak, S Suessner, W Holnthoner, H Redl, S Wolbank, E Priglinger
Eur Cell Mater, 2019-02-22;37(0):113-133.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Transcriptome network analysis identifies protective role of the LXR/SREBP-1c axis in murine pulmonary fibrosis
Authors: S Shichino, S Ueha, S Hashimoto, M Otsuji, J Abe, T Tsukui, S Deshimaru, T Nakajima, M Kosugi-Kan, FH Shand, Y Inagaki, H Shimano, K Matsushima
JCI Insight, 2019-01-10;4(1):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Flow Cytometry -
Lung fibroblasts express a miR-19a-19b-20a sub-cluster to suppress TGF-?-associated fibroblast activation in murine pulmonary fibrosis
Authors: K Souma, S Shichino, S Hashimoto, S Ueha, T Tsukui, T Nakajima, HI Suzuki, FHW Shand, Y Inagaki, T Nagase, K Matsushima
Sci Rep, 2018-11-09;8(1):16642.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Mutational analysis of AKT1 and PIK3CA in intraductal papillomas of the breast with special reference to cellular components
Authors: C Mishima, N Kagara, JI Ikeda, E Morii, T Miyake, T Tanei, Y Naoi, M Shimoda, K Shimazu, SJ Kim, S Noguchi
Am. J. Pathol., 2018-02-16;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Fibroblast Heterogeneity and Immunosuppressive Environment in Human Breast Cancer
Authors: A Costa, Y Kieffer, A Scholer-Da, F Pelon, B Bourachot, M Cardon, P Sirven, I Magagna, L Fuhrmann, C Bernard, C Bonneau, M Kondratova, I Kuperstein, A Zinovyev, AM Givel, MC Parrini, V Soumelis, A Vincent-Sa, F Mechta-Gri
Cancer Cell, 2018-02-15;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: FACS -
High-throughput immunophenotypic characterization of bone marrow- and cord blood-derived mesenchymal stromal cells reveals common and differentially expressed markers: identification of angiotensin-converting enzyme (CD143) as a marker differentially expressed between adult and perinatal tissue sources
Authors: E Amati, O Perbellini, G Rotta, M Bernardi, K Chieregato, S Sella, F Rodeghiero, M Ruggeri, G Astori
Stem Cell Res Ther, 2018-01-16;9(1):10.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Endothelial-to-mesenchymal transition drives atherosclerosis progression.
Authors: Chen PY, Qin L, Baeyens N et al.
J Clin Invest
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