Human alpha -Taxilin Antibody

Catalog #: AF5575 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5575
AF5575-SP

Detection of Human alpha -Taxilin by Western Blot.

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Human alpha -Taxilin Antibody Summary

Species Reactivity

Human

Specificity

Detects human alpha -Taxilin in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human alpha -Taxilin
Met1-Lys162
Accession # P40222

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human alpha -Taxilin by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human a-Taxilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5575) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for a-Taxilin at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunocytochemistry

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alpha ‑Taxilin in HepG2 Human Cell Line. a-Taxilin was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Sheep Anti-Human a-Taxilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5575) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha-Taxilin

alpha ‑Taxilin (TXLN‑ alpha ; also p75 [in rat] and IL‑14 alpha ) is the founding member of the taxilin family of proteins. Although its predicted MW is 62 kDa, it runs anomalously at 74‑76 kDa in SDS‑PAGE. alpha ‑Taxilin is ubiquitously expressed and participates in intracellular vesicle trafficking. In particular, alpha ‑Taxilin interacts with syntaxin family members that localize to the plasma membrane, providing for SNARE complex formation. alpha ‑Taxilin also regulates the placement of growing protein chains. Human alpha ‑Taxilin is 546 amino acids (aa) in length. Its defining characteristic is a large coiled‑coil region between aa 186‑491. When an alternate start site at Met176 is used, a secreted cytokine is generated termed IL‑14 alpha. Over aa 1‑162, human alpha ‑Taxilin shares 81% aa identity with mouse alpha ‑Taxilin.

Entrez Gene IDs

200081 (Human); 109658 (Mouse)

Alternate Names

alphaTaxilin; alpha-Taxilin; DKFZp451J0118; IL14; IL-14; interleukin 14; MGC118870; MGC118871; RP4-622L5.4; taxilin alpha; TXLN; TXLNA