Human alpha 1-Microglobulin Antibody Summary
Gly20-Val203
Accession # P02760
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human alpha 1‑Microglobulin by Western Blot. Western blot shows lysates of human liver tissue, Hep3B human hepatocellular carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and Huh-7 human hepatoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human a1-Microglobulin Monoclonal Antibody (Catalog # MAB7724) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for a1-Microglobulin at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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alpha 1-Microglobulin in HepG2 Human Cell Line. a1-Microglobulin was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human a1-Microglobulin Monoclonal Antibody (Catalog # MAB7724) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: alpha 1-Microglobulin
Human alpha 1-Microglobulin (alpha1-m/A1M; also protein HC) is a secreted, 31-32 kDa glycoprotein member of the lipocalin family, calycin superfamily of molecules. It is expressed by hepatocytes, keratinocytes, and endodermal derivatives in the embryo. alpha 1-Microglobulin appears to act as a heme scavenger, protecting cells and collagen against oxidative damage. It also acts as an immunosuppressant, inhibiting polyclonal lymphocyte activation and dampening granulocyte migration in response to chemokines. alpha 1-Microglobulin circulates either as a monomer, or bound to IgA, albumin or prothrombin. Human alpha 1-Microglobulin is generated through cleavage of a precursor molecule termed AMBP. This AMBP should not be confused with AMBP-1, a 120-140 kDa adrenomedullin-binding protein that is also known as Complement Factor H. The AMBP precursor contains a 19 aa signal sequence, an N-terminal 183 aa alpha 1-Microglobulin protein (aa 20-203), and a C-terminal serine protease inhibitor termed bikunin (aa 206-352). alpha 1-Microglobulin possesses one lipocalin domain (aa 42-186). Although cleavage of AMBP in the Golgi apparatus typically generates a 31 kDa alpha 1-Microglobulin and 28 kDa bikunin molecule, the 60-65 kDa AMBP precursor can also be released intact. alpha 1-Microglobulin will undergo extracellular processing, generating a 30 kDa isoform that is missing aa 199-203. There is one splice variant that shows a deletion of aa 48-57. Over aa 20-203, human alpha 1-Microglobulin shares 76% aa sequence identity with both mouse and rat alpha 1-Microglobulin.
Product Datasheets
Citations for Human alpha 1-Microglobulin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Are Mannan-binding Lectine Serin Protease-2 and Alpha-1-microglobulin and Bukinin Precursor the Potential Biomarkers of Manic Episode? A Study via Urinary Proetomic Analysis
Authors: Cerit C, Sarihan M, Nart O et al.
Clinical psychopharmacology and neuroscience : the official scientific journal of the Korean College of Neuropsychopharmacology
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A Retrospective Analysis of the Cartilage Kunitz Protease Inhibitory Proteins Identifies These as Members of the Inter-?-Trypsin Inhibitor Superfamily with Potential Roles in the Protection of the Articulatory Surface
Authors: SM Smith, J Melrose
Int J Mol Sci, 2019-01-24;20(3):.
Species: Ovine
Sample Types: Tissue Homogenates
Applications: Western Blot
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