Home / Angiopoietin-like Protein 4/ANGPTL4 / Human Angiopoietin-like 4 DuoSet ELISA

Human Angiopoietin-like 4 DuoSet ELISA

Catalog #: DY3485
36 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DY3485
Ancillary Products Available
DY008B: DuoSet ELISA Ancillary Reagent Kit 2
DY999B: Substrate Reagent Pack
DY999: Substrate Reagent Pack
Human Angiopoietin-like Protein 4 / ANGPTL4 ELISA Standard Curve
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Product Details
Procedure
Citations (36)
FAQs
Supplemental Products
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Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Human Angiopoietin-like 4 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
1.3 - 80 ng/mL
Sufficient Materials
For fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Angiopoietin-like4. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data

N/A Human Angiopoietin-like Protein 4 / ANGPTL4 ELISA Standard Curve View Larger

Human Angiopoietin-like Protein 4 / ANGPTL4 ELISA Standard Curve

N/A Human Angiopoietin-like 4 DuoSet ELISA View Larger

Human Angiopoietin-like 4 DuoSet ELISA Levels of ANGPTL4 in sera from RA patients and controls after correction for the false positive reactions to rheumatoid factor (RF).(A) ANGPTL4 was measured in sera from 57 patients with rheumatoid arthritis (RA) and 43 matched controls by ELISA using DY3485 kit with lot IV detection antibody. (B) ANGPTL4 was measured as in (A) in sera from RF+ (n = 33) and RF- (n = 20) patients with RA. The means ± SD are indicated. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28107351), licensed under a CC-BY license. Not internally tested by R&D Systems.

Product Datasheets

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Product Datasheet
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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Angiopoietin-like Protein 4/ANGPTL4

Angiopoietin-like, also known as angiopoietin-related proteins, are structurally related to the angiopoietins. They are secreted proteins with N-terminal coiled-coil domains and C-terminal fibrinogen-like domains. Unlike the angiopoietins, ANGPTLs do not bind Tie-2. Seven ANGPTL proteins have been identified (ANGPTL1 through 7).

Entrez Gene IDs:
51129 (Human); 57875 (Mouse); 362850 (Rat)
Alternate Names:
Angiopoietin like Protein 4; angiopoietin-like 4; Angiopoietin-like Protein 4; ANGPTL4; ARP4fasting-induced adipose factor; FIAF; FIAFhepatic angiopoietin-related protein; Hepatic fibrinogen/angiopoietin-related protein; HFARP; HFARPANGPTL2; NL2; peroxisome proliferator-activated receptor (PPAR) gamma inducedangiopoietin-related protein; PGAR; PGARangiopoietin-related protein 4; pp1158; PPARG angiopoietin related protein

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

Citations for Human Angiopoietin-like 4 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

36 Citations: Showing 1 - 10
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  1. Suppression of angiopoietin-like 4 reprograms endothelial cell metabolism and inhibits angiogenesis
    Authors: Chaube, B;Citrin, KM;Sahraei, M;Singh, AK;de Urturi, DS;Ding, W;Pierce, RW;Raaisa, R;Cardone, R;Kibbey, R;Fernández-Hernando, C;Suárez, Y;
    Nature communications
    Species: Human
    Sample Types: Cell Cultue Supernates, Cell Lysates
  2. Oleic acid-induced metastasis of KRAS/p53-mutant colorectal cancer relies on concurrent KRAS activation and IL-8 expression bypassing EGFR activation
    Authors: Shen, CJ;Chan, RH;Lin, BW;Li, NC;Huang, YH;Chang, WC;Chen, BK;
    Theranostics
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Hypoxia induced responses are reflected in the stromal proteome of breast cancer
    Authors: Kjølle, S;Finne, K;Birkeland, E;Ardawatia, V;Winge, I;Aziz, S;Knutsvik, G;Wik, E;Paulo, JA;Vethe, H;Kleftogiannis, D;Akslen, LA;
    Nature communications
    Species: Human
    Sample Types: Cell Culture Supernates
  4. Angiopoietin-like 4 induces head and neck squamous cell carcinoma cell migration through the NRP1/ABL1/PXN pathway
    Authors: Hefni, E;Menon, D;Ma, T;Asiedu, EB;Sultan, A;Meiller, T;Schneider, A;Sodhi, A;Montaner, S;
    Cellular signalling
    Species: Human
    Sample Types: Cell Culture Supernates
  5. Cancer-associated mesothelial cell-derived ANGPTL4 and STC1 promote the early steps of ovarian cancer metastasis
    Authors: P Bajwa, K Kordylewic, A Bilecz, RR Lastra, K Wroblewski, Y Rinkevich, E Lengyel, HA Kenny
    JCI Insight, 2023-03-22;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  6. HIF-1alpha accumulation in response to transient hypoglycemia may worsen diabetic eye disease
    Authors: C Guo, M Deshpande, Y Niu, I Kachwala, M Flores-Bel, H Megarity, T Nuse, S Babapoor-F, M Ramada, J Sanchez, N Inamdar, TV Johnson, MV Canto-Sole, S Montaner, A Sodhi
    Cell Reports, 2023-01-10;42(1):111976.
    Species: Human
    Sample Types: Cell Culture Supernates
  7. ANGPTL4, IL-6 and TNF-alpha as regulators of lipid metabolism during a marathon run
    Authors: M Górecka, K Krzemi?ski, T Mikulski, AW Ziemba
    Scientific Reports, 2022-11-19;12(1):19940.
    Species: Human
    Sample Types: Plasma
  8. ANGPTL4 influences the therapeutic response of neovascular age-related macular degeneration patients by promoting choroidal neovascularization
    Authors: Y Qin, A Dinabandhu, X Cao, JC Sanchez, K Jee, M Rodrigues, C Guo, J Zhang, J Vancel, D Menon, NS Khan, T Ma, SY Tzeng, YJ Daoud, JJ Green, GL Semenza, S Montaner, A Sodhi
    JCI Insight, 2022-07-08;0(0):.
    Species: Human
    Sample Types: Aqueous Humor
  9. GALNT2 rs4846914 SNP Is Associated with Obesity, Atherogenic Lipid Traits, and ANGPTL3 Plasma Level
    Authors: M Qaddoumi, P Hebbar, M Abu-Farha, A Al Somaly, M Melhem, F Al-Kayal, I AlKhairi, P Cherian, M Alanbaei, F Al-Mulla, J Abubaker, TA Thanaraj
    Genes, 2022-07-04;13(7):.
    Species: Human
    Sample Types: Plasma
  10. ANGPTL4 Expression Is Increased in Epicardial Adipose Tissue of Patients with Coronary Artery Disease
    Authors: Y Katanasaka, A Saito, Y Sunagawa, N Sari, M Funamoto, S Shimizu, K Shimizu, T Akimoto, C Ueki, M Kitano, K Hasegawa, G Sakaguchi, T Morimoto
    Journal of Clinical Medicine, 2022-04-27;11(9):.
    Species: Human
    Sample Types: Pericardial Fluid
  11. Circulating ANGPTL3 and ANGPTL4 levels predict coronary artery atherosclerosis severity
    Authors: T Sun, W Zhan, L Wei, Z Xu, L Fan, Y Zhuo, C Wang, J Zhang
    Lipids in Health and Disease, 2021-11-06;20(1):154.
    Species: Human
    Sample Types: Plasma
  12. Decreased ANGPTL4 impairs endometrial angiogenesis during peri-implantation period in patients with recurrent implantation failure
    Authors: M Li, J Hu, L Yao, M Gao
    J. Cell. Mol. Med., 2020-08-03;0(0):.
    Species: Human
    Sample Types: Serum
  13. Fasting induces ANGPTL4 and reduces LPL activity in human adipose tissue
    Authors: PMM Ruppert, CCJR Michielsen, EJ Hazebroek, A Pirayesh, G Olivecrona, LA Afman, S Kersten
    Mol Metab, 2020-06-03;40(0):101033.
    Species: Human
    Sample Types: Plasma
  14. Hypocholesterolemia and dysregulated production of angiopoietin-like proteins in sickle cell anemia patients
    Authors: F Vendrame, L Olops, STO Saad, FF Costa, KY Fertrin
    Cytokine, 2019-05-01;120(0):88-91.
    Species: Human
    Sample Types: Serum
  15. Induction of paracrine signaling in metastatic melanoma cells by PPAR? agonist rosiglitazone activates stromal cells and enhances tumor growth
    Authors: C Pich, P Meylan, B Mastelic-G, NT Nguyen, R Loyon, BK Trang, H Moser, C Moret, C Goepfert, J Hafner, MP Levesque, P Romero, C Jandus, L Michalik
    Cancer Res., 2018-09-05;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  16. Genetic inactivation of ANGPTL4 improves glucose homeostasis and is associated with reduced risk of diabetes
    Authors: V Gusarova, C O'Dushlain, TM Teslovich, PN Benotti, T Mirshahi, O Gottesman, CV Van Hout, MF Murray, A Mahajan, JB Nielsen, L Fritsche, AB Wulff, DF Gudbjartss, M Sjögren, CA Emdin, RA Scott, WJ Lee, A Small, LC Kwee, OP Dwivedi, RB Prasad, S Bruse, AE Lopez, J Penn, A Marcketta, JB Leader, CD Still, HL Kirchner, UL Mirshahi, AH Wardeh, CM Hartle, L Habegger, SN Fetterolf, T Tusie-Luna, AP Morris, H Holm, V Steinthors, P Sulem, U Thorsteins, JI Rotter, LM Chuang, S Damrauer, D Birtwell, CM Brummett, AV Khera, P Natarajan, M Orho-Melan, J Flannick, LA Lotta, CJ Willer, OL Holmen, MD Ritchie, DH Ledbetter, AJ Murphy, IB Borecki, JG Reid, JD Overton, O Hansson, L Groop, SH Shah, WE Kraus, DJ Rader, YI Chen, K Hveem, NJ Wareham, S Kathiresan, O Melander, K Stefansson, BG Nordestgaa, A Tybjærg-Ha, GR Abecasis, D Altshuler, JC Florez, M Boehnke, MI McCarthy, GD Yancopoulo, DJ Carey, AR Shuldiner, A Baras, FE Dewey, J Gromada
    Nat Commun, 2018-06-13;9(1):2252.
    Species: Human
    Sample Types: Plasma
  17. Angiopoietin-like 4 promotes osteosarcoma cell proliferation and migration and stimulates osteoclastogenesis
    Authors: T Zhang, A Kastrenopo, Q Larrouture, NA Athanasou, HJ Knowles
    BMC Cancer, 2018-05-08;18(1):536.
    Species: Human
    Sample Types: Cell Culture Supernates
  18. Hypoxia-Inducible Factor-Dependent Expression of Angiopoietin-Like 4 by Conjunctival Epithelial Cells Promotes the Angiogenic Phenotype of Pterygia
    Authors: Q Meng, Y Qin, M Deshpande, F Kashiwabuc, M Rodrigues, Q Lu, H Ren, JH Elisseeff, GL Semenza, SV Montaner, A Sodhi
    Invest. Ophthalmol. Vis. Sci., 2017-09-01;58(11):4514-4523.
    Species: Human
    Sample Types: Cell Culture Supernates
  19. Expression of the angiogenic mediator, angiopoietin-like 4, in the eyes of patients with proliferative sickle retinopathy
    Authors: K Jee, M Rodrigues, F Kashiwabuc, BP Applewhite, I Han, G Lutty, MF Goldberg, GL Semenza, S Montaner, A Sodhi
    PLoS ONE, 2017-08-23;12(8):e0183320.
    Species: Human
    Sample Types: Aqueous Humor
  20. Angiopoietin-like protein 3 and 4 expression 4 and their serum levels in hepatocellular carcinoma
    Authors: AS El-Shal, HE Zidan, NM Rashad, FM Wadea
    Cytokine, 2017-03-31;96(0):75-86.
    Species: Human
    Sample Types: Serum
  21. Angptl4 does not control hyperglucagonemia or �-cell hyperplasia following glucagon receptor inhibition
    Authors: H Okamoto, K Cavino, E Na, E Krumm, S Kim, PE Stevis, J Harp, AJ Murphy, GD Yancopoulo, J Gromada
    Proc. Natl. Acad. Sci. U.S.A, 2017-01-31;0(0):.
    Species: Human
    Sample Types: Plasma
  22. MicroRNA and mRNA Transcriptome Profiling in Primary Human Astrocytes Infected with Borrelia burgdorferi
    Authors: T Casselli, H Qureshi, E Peterson, D Perley, E Blake, B Jokinen, A Abbas, S Nechaev, JA Watt, A Dhasarathy, CA Brissette
    PLoS ONE, 2017-01-30;12(1):e0170961.
    Species: Human
    Sample Types: Cell Culture Supernates
  23. High Concentrations of Angiopoietin-Like Protein 4 Detected in Serum from Patients with Rheumatoid Arthritis Can Be Explained by Non-Specific Antibody Reactivity
    Authors: E Makoveichu, T Ruge, S Nilsson, A S”dergren, G Olivecrona
    PLoS ONE, 2017-01-20;12(1):e0168922.
    Species: Human
    Sample Types: Serum
  24. Angiopoietin-like protein 4 and cardiovascular function in COPD
    Authors: S Janciauski
    BMJ Open Respir Res, 2016-10-31;3(1):e000161.
    Species: Human
    Sample Types: Serum
  25. alpha1-Antitrypsin Combines with Plasma Fatty Acids and Induces Angiopoietin-like Protein 4 Expression.
    Authors: Frenzel E, Wrenger S, Brugger B, Salipalli S, Immenschuh S, Aggarwal N, Lichtinghagen R, Mahadeva R, Marcondes A, Dinarello C, Welte T, Janciauskiene S
    J Immunol, 2015-09-11;195(8):3605-16.
    Species: Human
    Sample Types: Cell Culture Supernates
  26. Cyclic strain alters the expression and release of angiogenic factors by human tendon cells.
    Authors: Mousavizadeh R, Khosravi S, Behzad H, McCormack R, Duronio V, Scott A
    PLoS ONE, 2014-05-13;9(5):e97356.
    Species: Human
    Sample Types: Cell Culture Supernates
  27. Acute-phase protein alpha1-antitrypsin--a novel regulator of angiopoietin-like protein 4 transcription and secretion.
    Authors: Frenzel E, Wrenger S, Immenschuh S, Koczulla R, Mahadeva R, Deeg H, Dinarello C, Welte T, Marcondes A, Janciauskiene S
    J Immunol, 2014-04-23;192(11):5354-62.
    Species: Human
    Sample Types: Cell Culture Supernates
  28. Involvement of angiopoietin-like 4 in matrix remodeling during chondrogenic differentiation of mesenchymal stem cells.
    Authors: Mathieu M, Iampietro M, Chuchana P, Guerit D, Djouad F, Noel D, Jorgensen C
    J Biol Chem, 2014-02-06;289(12):8402-12.
    Species: Human
    Sample Types: Cell Culture Supernates
  29. Host biomarkers distinguish dengue from leptospirosis in Colombia: a case-control study.
    Authors: Conroy A, Gelvez M, Hawkes M, Rajwans N, Liles W, Villar-Centeno L, Kain K
    BMC Infect Dis, 2014-01-20;14(0):35.
    Species: Human
    Sample Types: Serum
  30. The cancer gene WWOX behaves as an inhibitor of SMAD3 transcriptional activity via direct binding.
    Authors: Ferguson B, Gao X, Zelazowski M, Lee J, Jeter C, Abba M, Aldaz C
    BMC Cancer, 2013-12-11;13(0):593.
    Species: Human
    Sample Types: Cell Culture Supernates
  31. Circulating angiopoietin-like 4 links proteinuria with hypertriglyceridemia in nephrotic syndrome.
    Authors: Clement L, Mace C, Avila-Casado C, Joles J, Kersten S, Chugh S
    Nat Med, 2013-12-08;20(1):37-46.
    Species: Human
    Sample Types: Plasma
  32. Epidermal growth factor receptor variant type III markedly accelerates angiogenesis and tumor growth via inducing c-myc mediated angiopoietin-like 4 expression in malignant glioma.
    Authors: Katanasaka Y, Kodera Y, Kitamura Y, Morimoto T, Tamura T, Koizumi F
    Mol Cancer, 2013-04-25;12(0):31.
    Species: Human
    Sample Types: Cell Culture Supernates
  33. Disrupting galectin-1 interactions with N-glycans suppresses hypoxia-driven angiogenesis and tumorigenesis in Kaposi's sarcoma.
    J. Exp. Med., 2012-10-01;209(11):1985-2000.
    Species: Human
    Sample Types: Cell Culture Supernates
  34. Inactivation of lipoprotein lipase occurs on the surface of THP-1 macrophages where oligomers of angiopoietin-like protein 4 are formed.
    Authors: Makoveichuk E, Sukonina V, Kroupa O, Thulin P, Ehrenborg E, Olivecrona T, Olivecrona G
    Biochem Biophys Res Commun, 2012-07-20;425(2):138-43.
    Species: Human
    Sample Types: Cell Culture Supernates
  35. Hypoxia-inducible factor regulates osteoclast-mediated bone resorption: role of angiopoietin-like 4.
    Authors: Knowles HJ, Cleton-Jansen AM, Korsching E, Athanasou NA
    FASEB J., 2010-07-28;24(12):4648-59.
    Species: Human
    Sample Types: Cell Culture Supernates
  36. Viral G protein-coupled receptor up-regulates Angiopoietin-like 4 promoting angiogenesis and vascular permeability in Kaposi's sarcoma.
    Authors: Ma T, Jham BC, Hu J
    Proc. Natl. Acad. Sci. U.S.A., 2010-07-26;107(32):14363-8.
    Species: Human
    Sample Types: Cell Culture Supernates

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