Home / Apolipoprotein A-I/ApoA1 / Human Apolipoprotein A-I/ApoA1 Antibody

Human Apolipoprotein A-I/ApoA1 Antibody

Catalog #: AF3664
10 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3664
AF3664-SP
Detection of Human Apolipoprotein A‑I/ApoA1 by Western Blot.
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Citations (10)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Apolipoprotein A-I/ApoA1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ApoA1 in direct ELISAs and Western blots. In Western blots, less than 1% cross‑reactivity with recombinant human (rh) ApoA2, rhApoB100, and rhApoC2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Human Plasma ApoA1 and E. coli-derived recombinant human ApoA1
Asp25-Gln267
Accession # P02647
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Apolipoprotein A-I/ApoA1 antibody by Western Blot. View Larger

Detection of Human Apolipoprotein A‑I/ApoA1 by Western Blot. Western blot shows human plasma. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Apolipoprotein A-I/ApoA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3664) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Apolipoprotein A-I/ApoA1 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human Apolipoprotein A-I/ApoA1 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human Apolipoprotein A‑I/ApoA1 by Simple WesternTM. Simple Western lane view shows lysates of human serum, loaded at 1:5000. A specific band was detected for Apolipoprotein A-I/ApoA1 at approximately 32 kDa (as indicated) using 5 µg/mL of Goat Anti-Human Apolipoprotein A-I/ApoA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3664) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Apolipoprotein A-I/ApoA1

ApoA1 is a major protein component of serum high-density lipoprotein (HDL) and is produced by the liver and small intestine. It is involved in reverse cholesterol transport from tissues to the liver. ApoA1 is synthesized as a 267 amino acid (aa) precursor from which a signal peptide and short propeptide are removed. Mature human ApoA1 shares 65% and 61% aa sequence identity with mouse and rat ApoA1, respectively.

Entrez Gene IDs
335 (Human); 11806 (Mouse); 25081 (Rat)
Alternate Names
Alp-1; APOA1; Apo-AI; apoA-I; Apolipoprotein A1; Apolipoprotein AI; Apolipoprotein A-I; Brp-14; Ltw-1; Lvtw-1; MGC117399; Sep-1; Sep-2

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Citations for Human Apolipoprotein A-I/ApoA1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Astrocyte- and Neuron-Derived Extracellular Vesicles from Alzheimer's Disease Patients Effect Complement-Mediated Neurotoxicity.
    Authors: Nogueras-Ortiz, C J, Mahairaki, V Et al.
    Cells
  2. Host biomarker-based quantitative rapid tests for detection and treatment monitoring of tuberculosis and COVID-19
    Authors: Pierneef L, van Hooij A, de Jong D et al.
    iScience
  3. Multi-omic profiling of the leukemic microenvironment shows bone marrow interstitial fluid is distinct from peripheral blood plasma
    Authors: Lorenz Nierves, Jian Guo, Siyuan Chen, Janice Tsui, Anuli C. Uzozie, Jonathan W. Bush et al.
    Experimental Hematology & Oncology
  4. Neuronal-Derived EV Biomarkers Track Cognitive Decline in Alzheimer’s Disease
    Authors: Erden Eren, Jeannie-Marie Leoutsakos, Juan Troncoso, Constantine G. Lyketsos, Esther S. Oh, Dimitrios Kapogiannis
    Cells
  5. Mitochondrial Electron Transport Chain Protein Abnormalities Detected in Plasma Extracellular Vesicles in Alzheimer's Disease
    Authors: PJ Yao, E Eren, EJ Goetzl, D Kapogianni
    Biomedicines, 2021-10-31;9(11):.
    Species: Human
    Sample Types: Extracellular
    Applications: Western Blot
  6. Uncovering temperature‐dependent extracellular vesicle secretion in breast cancer
    Authors: Kurataka Otsuka, Yusuke Yamamoto, Takahiro Ochiya
    Journal of Extracellular Vesicles
  7. Serum amyloid A is a soluble pattern recognition receptor that drives type 2 immunity
    Authors: U Smole, N Gour, J Phelan, G Hofer, C Köhler, B Kratzer, PA Tauber, X Xiao, N Yao, J Dvorak, L Caraballo, L Puerta, S Rosskopf, J Chakir, E Malle, AP Lane, WF Pickl, S Lajoie, M Wills-Karp
    Nat. Immunol., 2020-06-22;21(7):756-765.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Immunoprecipitation
  8. Adenosine leakage from perforin-burst extracellular vesicles inhibits perforin secretion by cytotoxic T-lymphocytes
    Authors: H Tadokoro, A Hirayama, R Kudo, M Hasebe, Y Yoshioka, J Matsuzaki, Y Yamamoto, M Sugimoto, T Soga, T Ochiya
    PLoS ONE, 2020-04-10;15(4):e0231430.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Application of new host biomarker profiles in quantitative point-of-care tests facilitates leprosy diagnosis in the field
    Authors: Anouk van Hooij, Susan van den Eeden, Renate Richardus, Elisa Tjon Kon Fat, Louis Wilson, Kees L.M.C. Franken et al.
    EBioMedicine
  10. Aspirin hydrolysis in plasma is a variable function of butyrylcholinesterase and platelet-activating factor acetylhydrolase 1b2 (PAFAH1b2).
    Authors: Zhou G, Marathe G, Hartiala J, Hazen S, Allayee H, Tang W, McIntyre T
    J Biol Chem, 2013-03-18;288(17):11940-8.
    Species: Human
    Sample Types: Plasma
    Applications: Western Blot

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