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Human APRIL/TNFSF13 Antibody

Catalog #: MAB5860
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB5860
MAB5860-SP
Cell Proliferation Induced by APRIL/TNFSF13 and Neutralization by Human APRIL/TNFSF13 Antibody.
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Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human APRIL/TNFSF13 Antibody Summary

Species Reactivity
Human
Specificity
Detects human APRIL/TNFSF13 in direct ELISAs. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse APRIL is observed, and no cross-reactivity with recombinant human BAFF is observed.
Source
Monoclonal Mouse IgG1 Clone # 670820
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human embryonic kidney cell line HEK293-derived recombinant human APRIL/TNFSF13
Ala105-Leu250
Accession # O75888
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Neutralization
Measured by its ability to neutralize APRIL/TNFSF13-induced proliferation in mouse B cells. The Neutralization Dose (ND50) is typically 0.15-0.75 ug/mL in the presence of 200 ng/mL Recombinant Human APRIL/TNFSF13.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by APRIL/TNFSF13 and Neutralization by Human APRIL/TNFSF13 Antibody. View Larger

Cell Proliferation Induced by APRIL/TNFSF13 and Neutralization by Human APRIL/TNFSF13 Antibody. Recombinant Human APRIL/TNFSF13 induces proliferation in mouse B cells in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human APRIL/TNFSF13 (200 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human APRIL/TNFSF13 Monoclonal Antibody (Catalog # MAB5860). The ND50 is typically 0.15-0.75 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: APRIL/TNFSF13

APRIL (a proliferation inducing ligand), also known as TNFSF13, TALL2, and TRDL1, is a member of the TNF ligand superfamily. It is synthesized as a 32 kDa type II transmembrane protein which is cleaved by furin in the Golgi to release a 17 kDa soluble molecule. Secreted APRIL consists almost entirely of a single TNF homology domain. Little or no transmembrane APRIL is expressed on the cell surface. Alternate splicing generates isoforms with short deletions at the N- or C‑terminus. Both APRIL and the closely related protein BAFF signal through the TNF superfamily receptors TACI and BCMA to promote cellular proliferation and protect from apoptosis. APRIL can form bioactive heterotrimers with BAFF. A bioactive cell surface-expressed protein known as TWEPRIL consists of the intracellular domain, transmembrane segment, and stalk region of TWEAK fused to the TNF homology domain of APRIL. Human APRIL shares 85% amino acid sequence identity with mouse and rat APRIL.

Long Name
A Proliferation-inducing Ligand
Entrez Gene IDs
8741 (Human); 69583 (Mouse); 287437 (Rat)
Alternate Names
A proliferation-inducing ligand; APRIL; APRILFLJ57090; CD256 antigen; CD256; TALL2; TALL-2; TNF- and APOL-related leukocyte expressed ligand 2; TNF-related death ligand 1; TNFSF13; TRDL1; TRDL-1; tumor necrosis factor (ligand) superfamily, member 13; tumor necrosis factor ligand superfamily member 13; tumor necrosis factor-like protein ZTNF2; tumor necrosis factor-related death ligand-1; ZTNF2

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Citations for Human APRIL/TNFSF13 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. BDNF belongs to the nurse-like cell secretome and supports survival of B chronic lymphocytic leukemia cells
    Authors: H Talbot, S Saada, E Barthout, PF Gallet, N Gachard, J Abraham, A Jaccard, D Troutaud, F Lalloué, T Naves, AL Fauchais, MO Jauberteau
    Sci Rep, 2020-07-28;10(1):12572.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  2. Nurse-like cells promote CLL survival through LFA-3/CD2 interactions
    Oncotarget, 2016-01-01;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Selective regulation of axonal growth from developing hippocampal neurons by tumor necrosis factor superfamily member APRIL.
    Authors: Osorio C, Chacon P, White M, Kisiswa L, Wyatt S, Rodriguez-Tebar A, Davies A
    Mol Cell Neurosci, 2014-01-18;59(0):24-36.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization

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