Home / APRIL/TNFSF13 / Human APRIL/TNFSF13 Biotinylated Antibody

Human APRIL/TNFSF13 Biotinylated Antibody

Catalog #: BAF884
2 Citations Datasheet / COA / SDS
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BAF884
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Citations (2)
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Human APRIL/TNFSF13 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human APRIL in Western blots. In this format, less than 1% cross-reactivity is observed with rhTRANCE, rhTNF-alpha, rhFas Ligand, rhTRAIL, rhGITR, and rhVEGI.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human APRIL
aa 110-250
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human APRIL/TNFSF13 (Catalog # 884-AP)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: APRIL/TNFSF13

APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, and TRDL1, is a member of the TNF ligand superfamily (1). APRIL is synthesized as a 32 kDa type II transmembrane protein which is cleaved by furin in the Golgi to release a 17 kDa soluble molecule (2, 3). Secreted APRIL consists almost entirely of a single TNF homology domain (2, 3). Little or no transmembrane APRIL is expressed on the cell surface (3). Alternate splicing generates isoforms with short deletions at the N- or C-terminus (4). Human APRIL shares 85% aa sequence identity with mouse and rat APRIL. Among TNF superfamily ligands, BAFF shows the greatest sequence homology with APRIL, and the two proteins exhibit overlapping biological activities. APRIL promotes cellular proliferation and protects from apoptosis in normal and transformed cells (3, 5‑7). It is present in elevated amounts in a wide variety of cancers primarily due to expression by tumor-infiltrating neutrophils (4, 5, 7‑9). Both APRIL and BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, and BAFF additionally functions through BAFF R (6, 10, 11). A stretch of basic amino acids at the N-terminus of APRIL is required for its interaction with heparan sulfate proteoglycans (HSPGs) (12, 13). Binding to HSPGs is independent of APRIL’s binding to TACI and BCMA (12, 13). Interaction with HSPGs serves to concentrate APRIL on the surface of cells, thereby favoring TACI- or BCMA-mediated effects (8, 9, 13). APRIL can form bioactive heterotrimers with BAFF, and these circulate in the serum of patients with rheumatic immune disorders (14). A bioactive protein known as TWE-PRIL consists of the intracellular domain, transmembrane segment, and stalk region of TWEAK fused to the TNF homology domain of APRIL (15). TWE-PRIL is expressed in monocytes and activated T cells, and in contrast to APRIL, it is presented on the cell surface (15).

References
  1. Planelles, L. et al. (2008) Curr. Mol. Med. 8:829.
  2. Hahne, M. et al. (1998) J. Exp. Med. 188:1185.
  3. Lopez-Fraga, M. et al. (2001) EMBO Reports 2:945.
  4. Kelly, K. et al. (2000) Cancer Res. 60:1021.
  5. Roth, W. et al. (2001) Cell Death Differ. 8:403.
  6. Yu, G. et al. (2000) Nat. Immunol. 1:252.
  7. He, B. et al. (2004) J. Immunol. 172:3268.
  8. Schwaller, J. et al. (2007) Blood 109:331.
  9. Mhawech-Fauceglia, P. et al. (2008) Eur. J. Cancer 44:2097.
  10. Day, E.S. et al. (2005) Biochemistry 44:1919.
  11. Bossen, C. et al. (2006) J. Biol. Chem. 281:13964.
  12. Hendriks, J. et al. (2005) Cell Death Differ. 12:637.
  13. Ingold, K. et al. (2005) J. Exp. Med. 201:1375.
  14. Roschke, V. et al. (2002) J. Immunol. 169:4314.
  15. Pradet-Balade, B. et al. (2002) EMBO J. 21:5711.
Long Name
A Proliferation-inducing Ligand
Entrez Gene IDs
8741 (Human); 69583 (Mouse); 287437 (Rat)
Alternate Names
A proliferation-inducing ligand; APRIL; APRILFLJ57090; CD256 antigen; CD256; TALL2; TALL-2; TNF- and APOL-related leukocyte expressed ligand 2; TNF-related death ligand 1; TNFSF13; TRDL1; TRDL-1; tumor necrosis factor (ligand) superfamily, member 13; tumor necrosis factor ligand superfamily member 13; tumor necrosis factor-like protein ZTNF2; tumor necrosis factor-related death ligand-1; ZTNF2

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Citations for Human APRIL/TNFSF13 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Host-microbe cross-talk governs amino acid chirality to regulate survival and differentiation of B cells
    Authors: M Suzuki, T Sujino, S Chiba, Y Harada, M Goto, R Takahashi, M Mita, K Hamase, T Kanai, M Ito, MK Waldor, M Yasui, J Sasabe
    Science Advances, 2021-03-03;7(10):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. Intestinal bacteria trigger T cell-independent immunoglobulin A(2) class switching by inducing epithelial-cell secretion of the cytokine APRIL.
    Authors: He B, Xu W, Santini PA
    Immunity, 2007-06-01;26(6):812-26.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

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