Human ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Biotinylated Antibody

Catalog #: BAF4494 Datasheet / COA / SDS

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BAF4494	In Stock	50 ug / $509

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All ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human ASAHL/N‑acylethanolamine-hydrolyzing Acid in Western blots. In Western blots, approximately 25% cross-reactivity with recombinant mouse ASAHL is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human ASAHL/N‑acylethanolamine-hydrolyzing Acid A isoform 1
Ser29-Lys359 (Val151Ile)
Accession # Q02083.3

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

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Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human N-acylethanolamine-hydrolyzing Acid Amidase/ASAHL (Catalog # 4494-AH)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase

The human NAAA gene encodes N-acylethanolamine-hydrolyzing acid amidase, also known as ASAH-like protein (ASAHL), a fatty acid amidase with maximal activity at acidic pH (1). ASAHL hydrolyzes a number of N-acyl ethanolamines, including N-myristoyl-, N-stearoyl, N-oleoyl, and N-arachidonoyl, but is most active against N‑palmitoylethanolamine (2). ASAHL is a member of the cholylglycine hydrolase family of enzymes, and is structurally similar to acid ceramidase (3). ASAHL is both a lysosomal and a secreted enzyme, and like acid ceramidase, has been observed to be proteolytically processed during maturation (3). ASAHL can be distinguished from anandamide amidohydrolase by its lack of inhibition by methyl arachidonyl fluorophosphonate (2).

Entrez Gene IDs

27163 (Human); 67111 (Mouse); 497009 (Rat)

Alternate Names

Acid ceramidase-like protein; ASAHL; ASAH-like protein; EC 3.5.1.-; NAAA; N-acylethanolamine acid amidase; Nacylethanolaminehydrolyzing Acid Amidase; N-acylethanolamine-hydrolyzing acid amidase; N-acylsphingosine amidohydrolase (acid ceramidase)-like; N-acylsphingosine amidohydrolase-like; PLT