Human beta 2-Microglobulin Parameter Assay Kit

Name: Human beta 2-Microglobulin Parameter Assay Kit
Brand: R&D Systems
SKU: KGE019
Rating: 4.5 (2 reviews)

Catalog #: KGE019
5 Citations 2 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
KGE019

Control Products Available

QC224: Parameter Immunoassay Control Set 916 for Human B2M

Best Seller

Human beta 2-Microglobulin Assay ELISA Standard Curve

2 Images

All beta 2-Microglobulin ELISAs

Product Details

Citations (5)

FAQs

Supplemental Products

Reviews (2)

Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human beta 2-Microglobulin Parameter Assay Kit Summary

Assay Length

1.5 hours

Sample Type & Volume Required Per Well

Serum (20 uL), EDTA Plasma (20 uL), Heparin Plasma (20 uL), Urine (10 uL)

Sensitivity

0.132 ug/mL

Assay Range

0.2 - 10 ug/mL (Serum, EDTA Plasma, Heparin Plasma, Urine)

Specificity

Measures Human beta 2M levels in various samples.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Parameter beta 2-Microglobulin Immunoassay is a 1.5 hour forward sequential competitive enzyme immunoassay designed to measure beta 2M in serum, plasma, and urine.

Precision

Intra-Assay Precision (Precision within an assay) Three controls of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three controls of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Serum, EDTA Plasma, Heparin Plasma, Urine

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (µg/mL)	1.14	3.19	6.41	0.953	3.1	6.17
Standard Deviation	0.086	0.189	0.383	0.175	0.293	0.496
CV%	7.5	5.9	6	18.4	9.5	8

Recovery

The recovery of human beta 2M spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	99	79-114
Heparin Plasma (n=4)	106	91-124
Serum (n=4)	101	85-122
Urine (n=4)	90	76-105

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human beta 2M were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

Scientific Data

N/A

View Larger

Human beta 2-Microglobulin Assay ELISA Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: beta 2-Microglobulin

beta 2-Microglobulin (B2M) forms the light chain of Class I MHC molecules. It has been measured in a variety of body fluids, including serum/plasma, saliva, CSF, and urine. B2M freely passes through the glomerular membrane, but it is nearly completely reabsorbed and degraded in proximal tubule cells. Clinically, serum B2M is elevated in rheumatoid arthritis, systemic lupus erythematosus, viral infections, and conditions associated with decreased glomerular filtration.

Entrez Gene IDs:

567 (Human); 12010 (Mouse); 24223 (Rat); 101867173 (Cynomolgus Monkey)

Alternate Names:

B2M; beta 2Microglobulin; beta 2-Microglobulin; beta chain of MHC class I molecules; beta-2-microglobin; beta-2-microglobulin

Related Research Areas

Cancer Biomarkers

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human beta 2-Microglobulin Parameter Assay Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
Filter your results:

Filter by:

The soluble VCAM-1 level is a potential biomarker predicting severe acute graft versus host disease after allogeneic hematopoietic cell transplantation
Authors: SK Heo, EK Noh, YJ Lee, Y Shin, Y Kim, HS Im, H Kim, SJ Koh, YJ Min, JC Jo, Y Choi
BMC Cancer, 2022-09-20;22(1):997.
Species: Human
Sample Types: Plasma
Stacking Machine Learning Algorithms for Biomarker-Based Preoperative Diagnosis of a Pelvic Mass
Authors: R Shaw, AE Lokshin, MC Miller, G Messerlian, RG Moore
Cancers, 2022-03-02;14(5):.
Species: Human
Sample Types: Serum
Urinary Beta-2-Microglobulin and Late Nephrotoxicity in Childhood Cancer Survivors
Authors: E Latoch, K Kono?czuk, K Taranta-Ja, K Muszy?ska-, M Sawicka, A Wasilewska, M Krawczuk-R
Journal of Clinical Medicine, 2021-11-13;10(22):.
Species: Human
Sample Types: Urine
Physiological Fitness and the Pathophysiology of Chronic Lymphocytic Leukemia (CLL)
Authors: A Sitlinger, MA Deal, E Garcia, DK Thompson, T Stewart, GA MacDonald, N Devos, D Corcoran, JS Staats, J Enzor, KJ Weinhold, DM Brander, JB Weinberg, DB Bartlett
Cells, 2021-05-11;10(5):.
Species: Human
Sample Types: Plasma
Multiple biomarker algorithms to predict epithelial ovarian cancer in women with a pelvic mass: Can additional makers improve performance?
Authors: RG Moore, A Blackman, MC Miller, K Robison, PA DiSilvestr, EE Eklund, R Strongin, G Messerlian
Gynecol. Oncol., 2019-04-13;0(0):.
Species: Human
Sample Types: Serum