Human BMP-4 MAb (Clone 66119) Summary
Ser293-Arg408
Accession # P12644
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Alkaline Phosphatase Production Induced by BMP‑4 and Neutralization by Human BMP‑4 Antibody. Recombinant Human BMP‑4 (Catalog # 314-BP) induces alkaline phosphatase production in the the C2C12 mouse myoblast cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human BMP‑4 (500 ng/mL) is neutralized (green line) by increasing concentrations of Human BMP‑4 Monoclonal Antibody (Catalog # MAB757). The ND50 is typically 1-3 µg/mL in the presence of heparin (2 µg/mL).
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BMP-4
BMP4 is a TGF beta superfamily ligand that is widely expressed from early embryogenesis through adulthood. It plays an important role in mesenchyme formation, epidermal determination, suppression of neural induction, the development of multiple organs, and tissue repair (1 5). The human BMP4 precursor contains a 273 amino acid (aa) propeptide and a 116 aa mature protein (6). Processing of the propeptide by furin or proprotein convertase 6 enables the formation of the mature disulfidelinked homodimeric BMP4 and facilitates its secretion. Similar intracellular processes may lead to the formation and recreation of BMP4/BMP7 disulfidelinked heterodimer (7 9). Mature human and mouse BMP4 share 98% aa sequence identity. Human BMP4 shares 85% aa sequence identity with human BMP2 and less than 50% with other human BMPs. Compared to BMP4 homodimers, BMP4/ BMP7 heterodimers exhibit a greater potency in inducing osteogenic differentiation (9). In Xenopus, the heterodimers can also induce the formation of mesoderm, whereas BMP4 homodimers only provide ventralizing signals for existing mesoderm (10). BMP4 signals through tetrameric complexes composed of type I (primarily Activin RIA or BMPRIA) and type II (primarily Activin RIIA or BMPRII) receptors (11, 12). The bioavailability of BMP4 is regulated by its interaction with multiple proteins and glycosaminoglycan (13 - 15).
- Zhang, P. et al., 2008, Blood 111:1933.
- Gambaro, K. et al., 2006, Cell Death Differ. 13:1075.
- Simic, P. and Vukicevic, S., 2005, Cytokine Growth Factor Rev. 16:299.
- Sadlon, T.J. et al., 2004, Stem Cells 22:457.
- Frank, D.B. et al., 2005, Circ. Res. 97:496.
- Wozney, J. et al., 1988, Science 242:1528.
- Cui, Y. et al., 1998, EMBO J. 17:4735.
- Cui, Y. et al., 2001, Genes Dev. 15:2797.
- Aono, A. et al., 1995, Biochem. Biophys. Res. Commun. 210:670.
- Nishimatsu, S. and G.H. Thomsen, 1998, Mech. Dev. 74:75.
- Chen, D. et al., 2004, Growth Factors 22:233.
- Lavery, K. et al., 2008, J. Biol. Chem. 283:20948.
- Rosen, V., 2006, Ann. N.Y. Acad. Sci. 1068:19.
- Jones, C.M. and J.C. Smith, 1998, Dev. Biol. 194:12.
- Takada, T. et al., 2003, J. Biol. Chem. 278:43229.
Product Datasheets
Citations for Human BMP-4 MAb (Clone 66119)
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
16
Citations: Showing 1 - 10
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Roles of Gremlin 1 and Gremlin 2 in regulating ovarian primordial to primary follicle transition
Authors: Eric E. Nilsson, Ginger Larsen, Michael K. Skinner
REPRODUCTION
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Comparison of newly developed anti-bone morphogenetic protein 4 llama-derived antibodies with commercially available BMP4 inhibitors
Authors: Silvia Calpe, Ana C. P. Correia, Maria del Carmen Sancho-Serra, Kausilia K. Krishnadath
mAbs
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Ibrutinib Inhibits Angiogenesis and Tumorigenesis in a BTK-Independent Manner
Authors: J Liu, Z Liu, J Zhang, X Chen, J Chen, L Sui, J Yu
Pharmaceutics, 2022-09-05;14(9):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Furin, a transcriptional target of NKX2-5, has an essential role in heart development and function
Authors: L Dupays, N Towers, S Wood, A David, DJ Stuckey, T Mohun
PLoS ONE, 2019-03-06;14(3):e0212992.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
A subtype of cancer-associated fibroblasts with lower expression of alpha-smooth muscle actin suppresses stemness through BMP4 in oral carcinoma
Authors: AK Patel, K Vipparthi, V Thatikonda, I Arun, S Bhattachar, R Sharan, P Arun, S Singh
Oncogenesis, 2018-10-05;7(10):78.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Contribution of three-dimensional architecture and tumor-associated fibroblasts to hepcidin regulation in breast cancer
Authors: N Blanchette, D Kita, A Konstorum, L Tesfay, D Lemler, P Hegde, KP Claffey, FM Torti, SV Torti
Oncogene, 2018-04-26;37(29):4013-4032.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Thrombospondin-1 (TSP-1), a new bone morphogenetic protein-2 and -4 (BMP-2/4) antagonist identified in pituitary cells
Authors: C Sallon, I Callebaut, I Boulay, J Fontaine, D Logeart-Av, C Henriquet, M Pugnière, X Cayla, P Monget, G Harichaux, V Labas, S Canepa, C Taragnat
J. Biol. Chem., 2017-07-26;0(0):.
Species: Human, Ovine
Sample Types: Cell Culture Supernates, Recombinant Protein
Applications: Immunoprecipitation, Western Blot -
A rapid and sensitive bioassay for the simultaneous measurement of multiple bone morphogenetic proteins. Identification and quantification of BMP4, BMP6 and BMP9 in bovine and human serum.
Authors: Herrera B, Inman GJ
BMC Cell Biol., 2009-03-19;10(0):20.
Species: Human
Sample Types: Recombinant Protein
Applications: Neutralization -
BMP4 regulation of human megakaryocytic differentiation is involved in thrombopoietin signaling.
Authors: Jeanpierre S, Nicolini FE, Kaniewski B, Dumontet C, Rimokh R, Puisieux A, Maguer-Satta V
Blood, 2008-07-29;112(8):3154-63.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Chordin-like 1, a bone morphogenetic protein-4 antagonist, is upregulated by hypoxia in human retinal pericytes and plays a role in regulating angiogenesis.
Authors: Kane R, Godson C, O'Brien C
Mol. Vis., 2008-06-20;14(0):1138-48.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Activation of the BMP canonical signaling pathway in human optic nerve head tissue and isolated optic nerve head astrocytes and lamina cribrosa cells.
Authors: Zode GS, Clark AF, Wordinger RJ
Invest. Ophthalmol. Vis. Sci., 2007-11-01;48(11):5058-67.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot -
Bone morphogenetic proteins promote gliosis in demyelinating spinal cord lesions.
Authors: Fuller ML, DeChant AK, Rothstein B, Caprariello A, Wang R, Hall AK, Miller RH
Ann. Neurol., 2007-09-01;62(3):288-300.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC-Fr -
Altered bone morphogenetic protein signalling in the Helicobacter pylori-infected stomach.
Authors: Bleuming SA, Kodach LL, Garcia Leon MJ, Richel DJ, Peppelenbosch MP, Reitsma PH, Hardwick JC, van den Brink GR
J. Pathol., 2006-06-01;209(2):190-7.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
BMPER, a novel endothelial cell precursor-derived protein, antagonizes bone morphogenetic protein signaling and endothelial cell differentiation.
Authors: Moser M, Binder O, Wu Y, Aitsebaomo J, Ren R, Bode C, Bautch VL, Conlon FL, Patterson C
Mol. Cell. Biol., 2003-08-01;23(16):5664-79.
Species: Human
Sample Types: Recombinant Protein
Applications: Neutralization -
Sonic hedgehog regulates gastric gland morphogenesis in man and mouse.
Authors: van den Brink GR, Hardwick JC, Tytgat GN, Brink MA, Ten Kate FJ, Van Deventer SJ, Peppelenbosch MP
Gastroenterology, 2001-08-01;121(2):317-28.
Species: Human, Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Bone morphogenetic protein 2 controls iron homeostasis in mice independent of Bmp6
Authors: Susanna Canali, Chia-Yu Wang, Kimberly B. Zumbrennen-Bullough, Abraham Bayer, Jodie L. Babitt
American Journal of Hematology
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