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Human BRCA1 N-Terminus Antibody

Catalog #: AF2210
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2210
AF2210-SP
BRCA1 in Human Breast Cancer Tissue.
2 Images
Product Details
Citations (2)
FAQs
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human BRCA1 N-Terminus Antibody Summary

Species Reactivity
Human
Specificity
Detects human BRCA1 N-Terminus in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human BRCA1 N-Terminus
Met1-Gly200
Accession # P38398
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Immunoprecipitation
3 µg/500 µg cell lysate
U2OS human osteosarcoma cell line and HeLa human cervical epithelial carcinoma cell line, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry BRCA1 antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

BRCA1 in Human Breast Cancer Tissue. BRCA1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 5 µg/mL Goat Anti-Human BRCA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2210) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western Blot Detection of Human BRCA1 antibody by Western Blot. View Larger

Detection of Human BRCA1 by Western Blot. Western blot shows lysates of U2OS human osteosarcoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-human BRCA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2210) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for BRCA1 at approximately 250 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BRCA1

The BRCA1 (BReast CAncer gene 1) tumor suppressor protein has many reported functions. In addition to mediating signal transduction in DNA damage and repair responses, BRCA1 regulates transcriptional activity and assists in the preservation of chromosomal stability. BRCA1 is one of the first proteins recruited to sites of DNA double-strand breaks, and serves as part of a scaffold for assembling other DNA damage response or repair factors.

Long Name
Breast Cancer 1
Entrez Gene IDs
672 (Human); 12189 (Mouse); 497672 (Rat)
Alternate Names
BRCA1; BRCAI; breast and ovarian cancer susceptibility protein 1; breast and ovarian cancer sususceptibility protein; breast cancer 1, early onset; breast cancer type 1 susceptibility protein; EC 6.3.2; EC 6.3.2.-; IRIS; PNCA4; PSCP; subunit 1

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Citations for Human BRCA1 N-Terminus Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Overactive BRCA1 Affects Presenilin 1 in Induced Pluripotent Stem Cell-Derived Neurons in Alzheimer's Disease
    Authors: M Wezyk, A Szybinska, J Wojsiat, M Szczerba, K Day, H Ronnholm, M Kele, M Berdynski, B Peplonska, JP Fichna, J Ilkowski, M Styczynska, A Barczak, M Zboch, A Filipek-Gl, K Bojakowski, M Skrzypczak, K Ginalski, M Kabza, I Makalowska, M Barcikowsk, U Wojda, A Falk, C Zekanowski
    J. Alzheimers Dis., 2018-01-01;62(1):175-202.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  2. BRCA1 mediates protein homeostasis through the ubiquitination of PERK and IRE1
    Authors: Robert Hromas, Gayathri Srinivasan, Ming Yang, Aruna Jaiswal, Taylor A. Totterdale, Linda Phillips et al.
    iScience

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