Human CA19-9 Antibody

Catalog #: MAB10625 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10625-100
MAB10625-SP

Detection of CA19-9 in Capan-2 Human Cell Line by Flow Cytometry.

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All CA19-9 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CA19-9 Antibody Summary

Species Reactivity

Human

Specificity

Detects Human CA19-9 in ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 1031516

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human CA19-9 from Capan-2 human pancreatic adenocarcinoma cell line

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

Capan-2 human pancreatic adenocarcinoma cell line

Immunocytochemistry

8-25 µg/mL

Immersion fixed Capan‑2 human pancreatic adenocarcinoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CA19-9 in Capan-2 Human Cell Line by Flow Cytometry. Capan-2 human pancreatic adenocarcinoma cell line was stained with Mouse Anti-Human CA19-9 Monoclonal Antibody (Catalog # MAB10625, filled histogram) or isotype control antibody (MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Staining was performed using our Staining Membrane-associated Proteins protocol.

Immunocytochemistry

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CA19-9 in Capan‑2 Human Cell Line. CA19-9 was detected in immersion fixed Capan‑2 human pancreatic adenocarcinoma cell line using Mouse Anti-Human CA19-9 Monoclonal Antibody (Catalog # MAB10625) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CA19-9

CA19-9 (sialylated Lewis a antigen) is a serum tumor marker for pancreatic cancer diagnosis. It is also used for monitoring therapy in patients with pancreatic adenocarcinoma. CA19-9 is synthesized by normal pancreatic and biliary ductal cells and by gastric, colon, endometrial and saliva epithelia. In a normal patient, it is present in small amounts in serum. The plasmatic levels of CA19-9 are greatly increased in neoplastic disease.

Long Name

Carbohydrate Antigen 19-9

Alternate Names

CA 19-9; CA19-9