Human Carbonic Anhydrase IX/CA9 Alexa Fluor® 700-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB2188N-100UG
Detection of Carbonic Anhydrase IX/CA9 in U‑87 MG Human Cell Line by Flow Cytometry.
1 Image
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews

Human Carbonic Anhydrase IX/CA9 Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Carbonic Anhydrase IX (CA9) in direct ELISAs. In direct ELISAs, this antibody does not cross-react with recombinant mouse (rm) CA9 or with rhCA1, 2, 3, 4, 5A, 6, 7, 8, 10, 12, 13, or 14.
Source
Monoclonal Mouse IgG2A Clone # 303123
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Carbonic Anhydrase IX
Pro59-Asp414
Accession # Q16790
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
 U-87 MG human glioblastoma/astrocytoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry View Larger

Detection of Carbonic Anhydrase IX/CA9 in U‑87 MG Human Cell Line by Flow Cytometry. U-87 MG human glioblastoma/astrocytoma cell line was stained with Mouse Anti-Human Carbonic Anhydrase IX/CA9 Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB2188N, filled histogram) or isotype control antibody (IC003N, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Carbonic Anhydrase IX/CA9

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1-3). Topics in the CA meeting (6th International Conference on the CAs, June 20-25, 2003; Slovakia) ranged from use of CAs as markers for tumor and hypoxia in clinic, as nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. CA9, also known as membrane antigen MN and renal cell carcinoma (RCC)-associated antigen G250, is a transmembrane enzyme expressed primarily in carcinoma cells. It is one of the best markers for hypoxia and for RCC (4, 5). rhCA9 corresponds to the extracellular portion of human CA9.

References
  1. Pastorek, J. et al. (1994) Oncogene 9:2877.
  2. Opavsky, R. et al. (1996) Genomics 33:480.
  3. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
  4. Kaluzova, M. et al. (2004) Mol. Cell Biol. 24:5757.
  5. Mukouyama, H. et al. (2004) Clin. Cancer Res. 10:1421.
Entrez Gene IDs
768 (Human); 230099 (Mouse)
Alternate Names
CA9; CAIX; CA-IX; Carbonate dehydratase IX; carbonic anhydrase 9; Carbonic Anhydrase IX; carbonic dehydratase; EC 4.2.1.1; G250; Membrane antigen MN; MN; P54/58N; PMW1; RCC; RCC-associated antigen G250; RCC-associated protein G250; Renal cell carcinoma-associated antigen G250

Product Datasheets

You must select a language.

x

Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

Citation for Human Carbonic Anhydrase IX/CA9 Alexa Fluor® 700-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Progressive immune dysfunction with advancing disease stage in renal cell carcinoma
    Authors: David A. Braun, Kelly Street, Kelly P. Burke, David L. Cookmeyer, Thomas Denize, Christina B. Pedersen et al.
    Cancer Cell

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human Carbonic Anhydrase IX/CA9 Alexa Fluor® 700-conjugated Antibody

There are currently no reviews for this product. Be the first to review Human Carbonic Anhydrase IX/CA9 Alexa Fluor® 700-conjugated Antibody and earn rewards!

Have you used Human Carbonic Anhydrase IX/CA9 Alexa Fluor® 700-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review