Home / Carbonic Anhydrase IX/CA9 / Human Carbonic Anhydrase IX/CA9 PE-conjugated Antibody

Human Carbonic Anhydrase IX/CA9 PE-conjugated Antibody

Catalog #: FAB2188P
6 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB2188P
Detection of Carbonic Anhydrase IX/CA9 in U‑87 MG Human Cell Line by Flow Cytometry.
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Product Details
Citations (6)
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Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Carbonic Anhydrase IX/CA9 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Carbonic Anhydrase IX (CA9) in direct ELISAs. In direct ELISAs, this antibody does not cross-react with recombinant mouse (rm) CA9 or with rhCA1, 2, 3, 4, 5A, 6, 7, 8, 10, 12, 13, or 14.
Source
Monoclonal Mouse IgG2A Clone # 303123
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Carbonic Anhydrase IX
Pro59-Asp414
Accession # Q16790
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Carbonic Anhydrase IX/CA9 antibody in U-87 MG Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Carbonic Anhydrase IX/CA9 in U‑87 MG Human Cell Line by Flow Cytometry. U-87 MG human glioblastoma/astrocytoma cell line was stained with Mouse Anti-Human Carbonic Anhydrase IX/CA9 PE-conjugated Monoclonal Antibody (Catalog # FAB2188P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Carbonic Anhydrase IX/CA9

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1-3). Topics in the CA meeting (6th International Conference on the CAs, June 20-25, 2003; Slovakia) ranged from use of CAs as markers for tumor and hypoxia in clinic, as nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. CA9, also known as membrane antigen MN and renal cell carcinoma (RCC)-associated antigen G250, is a transmembrane enzyme expressed primarily in carcinoma cells. It is one of the best markers for hypoxia and for RCC (4, 5). rhCA9 corresponds to the extracellular portion of human CA9.

References
  1. Pastorek, J. et al. (1994) Oncogene 9:2877.
  2. Opavsky, R. et al. (1996) Genomics 33:480.
  3. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
  4. Kaluzova, M. et al. (2004) Mol. Cell Biol. 24:5757.
  5. Mukouyama, H. et al. (2004) Clin. Cancer Res. 10:1421.
Entrez Gene IDs
768 (Human); 230099 (Mouse)
Alternate Names
CA9; CAIX; CA-IX; Carbonate dehydratase IX; carbonic anhydrase 9; Carbonic Anhydrase IX; carbonic dehydratase; EC 4.2.1.1; G250; Membrane antigen MN; MN; P54/58N; PMW1; RCC; RCC-associated antigen G250; RCC-associated protein G250; Renal cell carcinoma-associated antigen G250

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Citations for Human Carbonic Anhydrase IX/CA9 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. The co-delivery of adenovirus-based immune checkpoint vaccine elicits a potent anti-tumor effect in renal carcinoma
    Authors: Nan Jiang, Yanyan Zheng, Jiage Ding, Jiawei Wang, Fei Zhu, Meng Wang et al.
    npj Vaccines
  2. Dual Antigen T Cell Engagers Targeting CA9 as an Effective Immunotherapeutic Modality for Targeting CA9 in Solid Tumors
    Authors: Nazanin Tatari, Xiaoyu Zhang, Shawn C. Chafe, Dillon McKenna, Keith A. Lawson, Minomi Subapanditha et al.
    Frontiers in Immunology
  3. Adenovirus-assembled DC vaccine induces dual-targeting CTLs for tumor antigen and adenovirus to eradicate tumors
    Authors: Ding, J;Zheng, Y;Zhu, F;Wang, M;Fang, L;Li, H;Tian, H;Liu, Y;Wang, G;Zheng, J;Chai, D;
    International immunopharmacology
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Absent in melanoma 2 enhances anti-tumour effects of CAIX promotor controlled conditionally replicative adenovirus in renal cancer
    Authors: D Chai, D Qiu, Z Zhang, S Yuchen Shi, G Wang, L Fang, H Li, H Li, H Tian, J Zheng
    J. Cell. Mol. Med., 2020-07-29;0(0):.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Combining DNA vaccine and AIM2 in H1 nanoparticles exert anti-renal carcinoma effects via enhancing tumor-specific multi-functional CD8+ T cell responses
    Authors: D Chai, H Shan, G Wang, Q Zhang, H Li, L Fang, J Song, N Liu, Q Zhang, H Yao, J Zheng
    Mol. Cancer Ther., 2018-11-06;0(0):.
    Species: Transgenic Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Renal tumouroids: challenges of manufacturing 3D cultures from patient derived primary cells
    Authors: Agata Nyga, Katerina Stamati, Patricia A. Redondo, Tayebeh Azimi, Andrew Feber, Joana B. Neves et al.
    Journal of Cell Communication and Signaling

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Reviews for Human Carbonic Anhydrase IX/CA9 PE-conjugated Antibody

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Human Carbonic Anhydrase IX/CA9 PE-conjugated Antibody
By Lucy Quach on 06/06/2018
Application: Flow Sample Tested: Cancer Cells Species: Human
Human Carbonic Anhydrase IX/CA9 PE-conjugated Antibody
By Anonymous on 08/24/2016
Application: Flow Sample Tested: Tumour tissue Species: Human