Home / CCL18/PARC / Human CCL18/PARC DuoSet ELISA

Human CCL18/PARC DuoSet ELISA

Catalog #: DY394
34 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DY394
DY394-05
Ancillary Products Available
DY008B: DuoSet ELISA Ancillary Reagent Kit 2
DY999B: Substrate Reagent Pack
DY999: Substrate Reagent Pack
Human CCL18 / PARC ELISA Standard Curve
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Product Details
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Citations (34)
FAQs
Supplemental Products
Reviews (1)
Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Human CCL18/PARC DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
7.8 - 500 pg/mL
Sufficient Materials
For fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human CCL18/PARC. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data

N/A Human CCL18 / PARC ELISA Standard Curve View Larger

Human CCL18 / PARC ELISA Standard Curve

Product Datasheets

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Product Datasheet
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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL18/PARC

Pulmonary and activation-regulated chemokine (PARC), also known as alternative macrophage activation-associated CC chemokine (AMAC)-1, macrophage inflammatory protein (MIP)-4, and dendritic cell chemokine (DC-CK1), is a novel CC chemokine that is highly homologous to MIP-1 alpha (61% amino acid sequence identity). In vitro, PARC mRNA expression is induced in alternatively activated macrophages by Th2 cytokines such as IL-4, IL-10 and IL-13, and inhibited by IFN-gamma.

Entrez Gene IDs:
6362 (Human)
Alternate Names:
Alternative macrophage activation-associated CC chemokine 1; AMAC-1; AMAC1CC chemokine ligand 18; AMAC-1Small-inducible cytokine A18; CCL18; chemokine (C-C motif) ligand 18 (pulmonary and activation-regulated); CKb7; DC-CK1; DC-CK1CC chemokine PARC; DCCK1Macrophage inflammatory protein 4; Dendritic cell chemokine 1; MIP4; MIP-4; MIP-4C-C motif chemokine 18; PARC; PARCPulmonary and activation-regulated chemokine; SCYA18chemokine (C-C), dendritic; small inducible cytokine A18; small inducible cytokine subfamily A (Cys-Cys), member 18, pulmonary andactivation-regulated

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

Citations for Human CCL18/PARC DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

34 Citations: Showing 1 - 10
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  1. The IL-4/13-induced production of M2 chemokines by human lung macrophages is enhanced by adenosine and PGE2
    Authors: Brollo, M;Salvator, H;Grassin-Delyle, S;Glorion, M;Descamps, D;Buenestado, A;Naline, E;Tenor, H;Tiotiu, A;Devillier, P;
    International immunopharmacology
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Prevention of M2 polarization and temporal limitation of differentiation in monocytes by extracellular ATP
    Authors: Scherr, BF;Reiner, MF;Baumann, F;Höhne, K;Müller, T;Ayata, K;Müller-Quernheim, J;Idzko, M;Zissel, G;
    BMC immunology
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Onchocerca volvulus-specific antibody and cellular responses in onchocerciasis patients treated annually with ivermectin for 30 years and exposed to parasite transmission in central Togo
    Authors: SI Johanns, RG Gantin, B Wangala, K Komlan, WA Halatoko, M Banla, P Karabou, AJ Luty, H Schulz-Key, C Köhler, PT Soboslay
    PloS Neglected Tropical Diseases, 2022-05-03;16(5):e0010340.
    Species: Human
    Sample Types: Cell Culture Supernates
  4. Echinococcus multilocularis specific antibody, systemic cytokine, and chemokine levels, as well as antigen-specific cellular responses in patients with progressive, stable, and cured alveolar echinococcosis: A 10-year follow-up
    Authors: B Grüner, L Peters, A Hillenbran, P Vo beta berg, J Schweiker, EG Rollmann, LH Rodriguez, J Blumhardt, S Burkert, P Kern, C Köhler, PT Soboslay
    PloS Neglected Tropical Diseases, 2022-02-02;16(2):e0010099.
    Species: Human
    Sample Types: Cell Culture Supernates
  5. Tumor-Derived Extracellular Vesicles Induce CCL18 Production by Mast Cells: A Possible Link to Angiogenesis
    Authors: I Shefler, P Salamon, T Zitman-Gal, YA Mekori
    Cells, 2022-01-21;11(3):.
    Species: Human
    Sample Types: Cell Culture Supernates
  6. Insights into immunometabolism: A dataset correlating the 18FDG PET/CT maximum standard uptake value of the primary tumor with the CCL18 serum level in non-small cell lung cancer
    Authors: T Dönmez, K Höhne, G Zissel, K Herrmann, H Hautzel, C Aigner, B Hegedüs, T Ploenes
    Data in Brief, 2021-02-18;35(0):106859.
    Species: Human
    Sample Types: Serum
  7. Reprogramming of profibrotic macrophages for treatment of bleomycin-induced pulmonary fibrosis
    Authors: F Zhang, EA Ayaub, B Wang, E Puchulu-Ca, YH Li, SU Hettiarach, SD Lindeman, Q Luo, S Rout, M Srinivasar, A Cox, K Tsoyi, C Nickerson-, IO Rosas, PS Low
    EMBO Mol Med, 2020-06-29;0(0):e12034.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. Genetic Variation in CCL18 Gene Influences CCL18 Expression and Correlates with Survival in Idiopathic Pulmonary Fibrosis-Part B
    Authors: C Caliskan, B Seeliger, B Jäger, J Fuge, T Welte, O Terwolbeck, J Freise, CHM van Moorse, Y Zhang, A Prasse
    J Clin Med, 2020-06-25;9(6):.
    Species: Human
    Sample Types: Serum
  9. Human interleukin-4-treated regulatory macrophages promote epithelial wound healing and reduce colitis in a mouse model
    Authors: TS Jayme, G Leung, A Wang, ML Workentine, S Rajeev, A Shute, BE Callejas, N Mancini, PL Beck, R Panaccione, DM McKay
    Sci Adv, 2020-06-05;6(23):eaba4376.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. Pancreatic cancer-derived small extracellular vesical Ezrin regulates macrophage polarization and promotes metastasis
    Authors: YT Chang, HY Peng, CM Hu, SC Huang, SC Tien, YM Jeng
    Am J Cancer Res, 2020-01-01;10(1):12-37.
    Species: Human
    Sample Types: Cell Culture Supernates
  11. Intrapulmonary Autoantibodies to HSP72 Are Associated with Improved Outcomes in IPF
    Authors: R Mills, A Mathur, LM Nicol, JJ Walker, AA Przybylski, AC Mackinnon, SEM Howie, WAH Wallace, I Dransfield, N Hirani
    J Immunol Res, 2019-04-11;2019(0):1845128.
    Species: Human
    Sample Types: Cell Culture Supernates
  12. Prominent Levels of the Profibrotic Chemokine CCL18 during Peritonitis: In Vitro Downregulation by Vitamin D Receptor Agonists
    Authors: M Ossorio, V Martínez, MA Bajo, G Del Peso, MJ Castro, S Romero, R Selgas, T Bellón
    Biomed Res Int, 2018-04-04;2018(0):6415892.
    Species: Human
    Sample Types: Cell Culture Supernates
  13. Distinct Properties of Human M-CSF and GM-CSF Monocyte-Derived Macrophages to Simulate Pathological Lung Conditions In Vitro: Application to Systemic and Inflammatory Disorders with Pulmonary Involvement
    Authors: A Lescoat, A Ballerie, Y Augagneur, C Morzadec, L Vernhet, O Fardel, P Jégo, S Jouneau, V Lecureur
    Int J Mol Sci, 2018-03-17;19(3):.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. Is serum level of CC chemokine ligand 18 a biomarker for the prediction of radiation induced lung toxicity (RILT)?
    Authors: E Gkika, W Vach, S Adebahr, T Schimeck-J, A Brenner, TB Brunner, K Kaier, A Prasse, J Müller-Que, AL Grosu, G Zissel, U Nestle
    PLoS ONE, 2017-09-28;12(9):e0185350.
    Species: Human
    Sample Types: Serum
  15. Image based Machine Learning for identification of macrophage subsets
    Authors: HM Rostam, PM Reynolds, MR Alexander, N Gadegaard, AM Ghaemmagha
    Sci Rep, 2017-06-14;7(1):3521.
    Species: Human
    Sample Types: Cell Culture Supernates
  16. Sustained low peritoneal effluent CCL18 levels are associated with preservation of peritoneal membrane function in peritoneal dialysis
    Authors: M Ossorio, MA Bajo, G Del Peso, V Martínez, M Fernández, MJ Castro, A Rodríguez-, R Madero, T Bellón, R Selgas
    PLoS ONE, 2017-04-17;12(4):e0175835.
    Species: Human
    Sample Types: Serum
  17. Validating glycoprotein non-metastatic melanoma B (gpNMB, osteoactivin), a new biomarker of Gaucher disease
    Authors: Vagishwari Murugesan
    Blood Cells Mol. Dis, 2016-12-13;0(0):.
    Species: Human
    Sample Types: Serum
  18. Exposure to diesel exhaust particle extracts (DEPe) impairs some polarization markers and functions of human macrophages through activation of AhR and Nrf2.
    Authors: Jaguin M, Fardel O, Lecureur V
    PLoS ONE, 2015-02-24;10(2):e0116560.
    Species: Human
    Sample Types: Cell Culture Supernates
  19. Macrophage activation in acute exacerbation of idiopathic pulmonary fibrosis.
    Authors: Schupp J, Binder H, Jager B, Cillis G, Zissel G, Muller-Quernheim J, Prasse A
    PLoS ONE, 2015-01-15;10(1):e0116775.
    Species: Human
    Sample Types: BALF
  20. Human B cells induce dendritic cell maturation and favour Th2 polarization by inducing OX-40 ligand.
    Authors: Maddur M, Sharma M, Hegde P, Stephen-Victor E, Pulendran B, Kaveri S, Bayry J
    Nat Commun, 2014-06-09;5(0):4092.
    Species: Human
    Sample Types: Cell Culture Supernates
  21. Proteome-wide analysis and CXCL4 as a biomarker in systemic sclerosis
    Authors: L van Bon, AJ Affandi, J Broen, RB Christmann, RJ Marijnisse, L Stawski, GA Farina, G Stifano, AL Mathes, M Cossu, M York, C Collins, M Wenink, R Huijbens, R Hesselstra, T Saxne, M DiMarzio, D Wuttge, SK Agarwal, JD Reveille, S Assassi, M Mayes, Y Deng, JP Drenth, J de Graaf, M den Heijer, CG Kallenberg, M Bijl, A Loof, WB van den Be, LA Joosten, V Smith, F de Keyser, R Scorza, C Lunardi, PL van Riel, M Vonk, W van Heerde, S Meller, B Homey, L Beretta, M Roest, M Trojanowsk, R Lafyatis, TR Radstake
    N. Engl. J. Med, 2013-12-18;370(5):433-43.
    Species: Human
    Sample Types: Plasma
  22. Lung collagens perpetuate pulmonary fibrosis via CD204 and M2 macrophage activation.
    Authors: Stahl M, Schupp J, Jager B, Schmid M, Zissel G, Muller-Quernheim J, Prasse A
    PLoS ONE, 2013-11-20;8(11):e81382.
    Species: Human
    Sample Types: Cell Culture Supernates
  23. Serum level of CC-chemokine ligand 18 is increased in patients with non-small-cell lung cancer and correlates with survival time in adenocarcinomas.
    Authors: Plones T, Krohn A, Burger M, Veelken H, Passlick B, Muller-Quernheim J, Zissel G
    PLoS ONE, 2012-07-25;7(7):e41746.
    Species: Human
    Sample Types: Serum
  24. Potential biomarkers of osteonecrosis in Gaucher disease.
    Authors: Pavlova EV, Deegan PB, Tindall J, McFarlane I, Mehta A, Hughes D, Wraith JE, Cox TM
    Blood Cells Mol. Dis., 2010-11-13;46(1):27-33.
    Species: Human
    Sample Types: Serum
  25. Interferon-gamma reverses the immunosuppressive and protumoral properties and prevents the generation of human tumor-associated macrophages.
    Authors: Duluc D, Corvaisier M, Blanchard S, Catala L, Descamps P, Gamelin E, Ponsoda S, Delneste Y, Hebbar M, Jeannin P
    Int. J. Cancer, 2009-07-15;125(2):367-73.
    Species: Human
    Sample Types: Cell Culture Supernates
  26. Mature dendritic cells express functional thrombin receptors triggering chemotaxis and CCL18/pulmonary and activation-regulated chemokine induction.
    Authors: Li X, Syrovets T, Paskas S, Laumonnier Y, Simmet T
    J. Immunol., 2008-07-15;181(2):1215-23.
    Species: Human
    Sample Types: Cell Culture Supernates
  27. Inorganic arsenic activates reduced NADPH oxidase in human primary macrophages through a Rho kinase/p38 kinase pathway.
    Authors: Lemarie A, Bourdonnay E, Morzadec C, Fardel O, Vernhet L
    J. Immunol., 2008-05-01;180(9):6010-7.
    Species: Human
    Sample Types: Cell Culture Supernates
  28. Tumor-associated leukemia inhibitory factor and IL-6 skew monocyte differentiation into tumor-associated macrophage-like cells.
    Authors: Duluc D, Delneste Y, Tan F, Moles MP, Grimaud L, Lenoir J, Preisser L, Anegon I, Catala L, Ifrah N, Descamps P, Gamelin E, Gascan H, Hebbar M, Jeannin P
    Blood, 2007-09-11;110(13):4319-30.
    Species: Human
    Sample Types: Cell Culture Supernates
  29. CCL18 as an indicator of pulmonary fibrotic activity in idiopathic interstitial pneumonias and systemic sclerosis.
    Authors: Prasse A, Pechkovsky DV, Toews GB, Schafer M, Eggeling S, Ludwig C, Germann M, Kollert F, Zissel G, Muller-Quernheim J
    Arthritis Rheum., 2007-05-01;56(5):1685-93.
    Species: Human
    Sample Types: Serum
  30. High CCL18/PARC expression in articular cartilage and synovial tissue of patients with rheumatoid arthritis.
    Authors: Momohara S, Okamoto H, Iwamoto T, Mizumura T, Ikari K, Kawaguchi Y, Takeuchi M, Kamatani N, Tomatsu T
    J. Rheumatol., 2007-02-01;34(2):266-71.
    Species: Human
    Sample Types: Synovial Fluid
  31. Induction of prolonged infiltration of T lymphocytes and transient T lymphocyte-dependent collagen deposition in mouse lungs following adenoviral gene transfer of CCL18.
    Authors: Luzina IG, Papadimitriou JC, Anderson R, Pochetuhen K, Atamas SP
    Arthritis Rheum., 2006-08-01;54(8):2643-55.
    Species: Human
    Sample Types: BALF
  32. Diesel exhaust exposure favors TH2 cell recruitment in nonatopic subjects by differentially regulating chemokine production.
    Authors: Chang Y, Senechal S, de Nadai P, Chenivesse C, Gilet J, Vorng H, Legendre B, Tonnel AB, Wallaert B, Lassalle P, Tsicopoulos A
    J. Allergy Clin. Immunol., 2006-06-21;118(2):354-60.
    Species: Human
    Sample Types: Cell Culture Supernates
  33. CCL18 is expressed in atopic dermatitis and mediates skin homing of human memory T cells.
    Authors: Gunther C, Bello-Fernandez C, Kopp T, Kund J, Carballido-Perrig N, Hinteregger S, Fassl S, Schwarzler C, Lametschwandtner G, Stingl G, Biedermann T, Carballido JM
    J. Immunol., 2005-02-01;174(3):1723-8.
    Species: Human
    Sample Types: Serum
  34. CC chemokine ligand 18, an atopic dermatitis-associated and dendritic cell-derived chemokine, is regulated by staphylococcal products and allergen exposure.
    Authors: Pivarcsi A, Gombert M, Dieu-Nosjean MC, Kubitza R, Meller S, Rieker J, Muller A, Da Cunha L, Haahtela A, Sonkoly E, Fridman WH, Alenius H, Kemeny L, Ruzicka T, Zlotnik A
    J. Immunol., 2004-11-01;173(9):5810-7.
    Species: Human
    Sample Types: Cell Culture Supernates

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Human CCL18/PARC DuoSet ELISA
By Anonymous on 05/22/2017
Sample Tested: Lung tissue