Human CCL2/MCP-1 DuoSet ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human CCL2/MCP-1. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.
Product Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Kit Content
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.
The components listed above may be purchased separately:
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4
Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990)
Plate Sealers: ELISA Plate Sealers (Catalog # DY992)
Scientific Data
Product Datasheets
Preparation and Storage
Background: CCL2/JE/MCP-1
CCL2/JE/MCP-1, is a chemokine that binds the receptor CCR2 and induces the chemoattraction of mononuclear cells. It induces the activation of monocytes, NK cells, lymphocytes, and basophils. Additionally, CCL2 promotes Th2 polarization in CD4+ T cells, and CCL2-mediated recruitment of monocytes to sites of inflammation contributes to disease severity in atherosclerosis, multiple sclerosis, and allergic asthma. Endogenous proteolytic trimming of CCL2 at the N-terminus, including the N-terminal pyrrolidone carboxylic acid-modified glutamine, downregulates activity but not receptor binding.
Assay Procedure
GENERAL ELISA PROTOCOL
Plate Preparation
- Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
- Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
- Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
- Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.
Assay Procedure
- Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
- Repeat the aspiration/wash as in step 2 of Plate Preparation.
- Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
- Repeat the aspiration/wash as in step 2 of Plate Preparation.
- Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
- Repeat the aspiration/wash as in step 2.
- Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
- Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
- Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.
Citations for Human CCL2/MCP-1 DuoSet ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Chemokine CCL2 and its receptor CCR2 in different age groups of patients with COVID-19
Authors: Bagheri, V;Khorramdelazad, H;Kafi, M;Abbasifard, M;
BMC immunology
Species: Human
Sample Types: Serum
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Gene expression responses of CF airway epithelial cells exposed to elexacaftor/tezacaftor/ivacaftor (ETI) suggest benefits beyond improved CFTR channel function
Authors: Hampton, TH;Barnaby, R;Roche, C;Nymon, A;Fukutani, KF;MacKenzie, TA;Stanton, BA;
bioRxiv : the preprint server for biology
Species: Human
Sample Types: Cell Culture Supernates
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Effect of compressive force combined with vibration on CCL2 and CCL5 in human periodontal ligament cells
Authors: Pongtiwattanakul, S;Leethanakul, C;Rattanaporn, O;Thammanichanon, P;Tannukit, S;
Journal of oral biology and craniofacial research
Species: Human
Sample Types: Cell Culture Supernates
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Landscape of Interactions between Stromal and Myeloid Cells in Ileal Crohn's Disease; Indications of an Important Role for Fibroblast-Derived CCL-2
Authors: Dovrolis, N;Valatas, V;Drygiannakis, I;Filidou, E;Spathakis, M;Kandilogiannakis, L;Tarapatzi, G;Arvanitidis, K;Bamias, G;Vradelis, S;Manolopoulos, VG;Paspaliaris, V;Kolios, G;
Biomedicines
Species: Human
Sample Types: Cell Culture Supernates
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Effect of Isoscopoletin on Cytokine Expression in HaCaT Keratinocytes and RBL-2H3 Basophils: Preliminary Study
Authors: Seo, DY;Park, JW;Kim, SH;Oh, SR;Han, SB;Kwon, OK;Ahn, KS;
International journal of molecular sciences
Species: Human
Sample Types: Cell Culture Supernates
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Nuclear factor-?B activation by transforming growth factor-?1 drives tumour microenvironment-mediated drug resistance in neuroblastoma
Authors: Louault, K;Blavier, L;Lee, MH;Kennedy, RJ;Fernandez, GE;Pawel, BR;Asgharzadeh, S;DeClerck, YA;
British journal of cancer
Species: Human
Sample Types: Cell Culture Supernates
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Association of the Reduced Levels of Monocyte Chemoattractant Protein-1 with Herpes Zoster in Rheumatoid Arthritis Patients Treated with Janus Kinase Inhibitors in a Single-Center Cohort
Authors: Chen, PK;Chen, YM;Chen, HH;Liao, TL;Chang, SH;Yeo, KJ;Huang, PH;Chen, DY;
Microorganisms
Species: Human
Sample Types: Plasma
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Targeting IL-6 trans-signalling by sgp130Fc attenuates severity in SARS-CoV-2 -infected mice and reduces endotheliopathy
Authors: Rodríguez-Hernández, MÁ;Baena-Bustos, M;Carneros, D;Zurita-Palomo, C;Muñoz-Pinillos, P;Millán, J;Padillo, FJ;Smerdou, C;von Kobbe, C;Rose-John, S;Bustos, M;
EBioMedicine
Species: Transgenic Mouse
Sample Types: Serum
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LHPP expression in triple-negative breast cancer promotes tumor growth and metastasis by modulating the tumor microenvironment
Authors: Reina, J;Vallmajo-Martin, Q;Ning, J;Michi, AN;Yeung, K;Wahl, GM;Hunter, T;
bioRxiv : the preprint server for biology
Species: Human
Sample Types: Cell Culture Supernates
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Delayed CCL23 response is associated with poor outcomes after cardiac arrest
Authors: deKay, JT;Chepurko, E;Chepurko, V;Knudsen, L;Lord, C;Searight, M;Tsibulnikov, S;Robich, MP;Sawyer, DB;Gagnon, DJ;May, T;Riker, R;Seder, DB;Ryzhov, S;
Cytokine
Species: Human
Sample Types: Plasma
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High Phosphate-Induced JAK-STAT Signalling Sustains Vascular Smooth Muscle Cell Inflammation and Limits Calcification
Authors: Macrì, F;Vigorito, I;Castiglione, S;Faggiano, S;Casaburo, M;Fanotti, N;Piacentini, L;Vigetti, D;Vinci, MC;Raucci, A;
Biomolecules
Species: Human
Sample Types: Cell Culture Supernates
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Macrophages derived from LPS-stimulated monocytes from individuals with subclinical atherosclerosis were characterized by increased pro-inflammatory activity
Authors: Nikiforov, NG;Kirichenko, TV;Kubekina, MV;Chegodaev, YS;Zhuravlev, AD;Ilchuk, LA;Nikolaeva, MA;Arefieva, AS;Popov, MA;Verkhova, SS;Bagheri Ekta, M;Orekhov, AN;
Cytokine
Species: Human
Sample Types: Cell Culture Supernates
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Human PBMCs Form Lipid Droplets in Response to Spike Proteins
Authors: Sivaraman, K;Pino, P;Raussin, G;Anchisi, S;Metayer, C;Dagany, N;Held, J;Wrenger, S;Welte, T;Wurm, MJ;Wurm, FM;Olejnicka, B;Janciauskiene, S;
Microorganisms
Species: Human
Sample Types: Cell Culture Supernates
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CD40 Ligand-CD40 Interaction Is an Intermediary between Inflammation and Angiogenesis in Proliferative Diabetic Retinopathy
Authors: Abu El-Asrar, AM;Nawaz, MI;Ahmad, A;Dillemans, L;Siddiquei, M;Allegaert, E;Gikandi, PW;De Hertogh, G;Opdenakker, G;Struyf, S;
International journal of molecular sciences
Species: Human
Sample Types: Cell Culture Supernates
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SARS-CoV-2 spike protein induces endothelial inflammation via ACE2 independently of viral replication
Authors: Montezano, AC;Camargo, LL;Mary, S;Neves, KB;Rios, FJ;Stein, R;Lopes, RA;Beattie, W;Thomson, J;Herder, V;Szemiel, AM;McFarlane, S;Palmarini, M;Touyz, RM;
Scientific reports
Species: Human
Sample Types: Cell Culture Supernates
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IL1R1+ cancer-associated fibroblasts drive tumor development and immunosuppression in colorectal cancer
Authors: Koncina, E;Nurmik, M;Pozdeev, VI;Gilson, C;Tsenkova, M;Begaj, R;Stang, S;Gaigneaux, A;Weindorfer, C;Rodriguez, F;Schmoetten, M;Klein, E;Karta, J;Atanasova, VS;Grzyb, K;Ullmann, P;Halder, R;Hengstschläger, M;Graas, J;Augendre, V;Karapetyan, YE;Kerger, L;Zuegel, N;Skupin, A;Haan, S;Meiser, J;Dolznig, H;Letellier, E;
Nature communications
Species: Human
Sample Types: Cell Culture Supernates
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Mild Cognitive Impairment Is Associated with Enhanced Activation of Th17 Lymphocytes in Non-Alcoholic Fatty Liver Disease
Authors: Fiorillo, A;Gallego, JJ;Casanova-Ferrer, F;Giménez-Garzó, C;Urios, A;Ballester, MP;Durbán, L;Rios, MP;Megías, J;San Miguel, T;Kosenko, E;Escudero-García, D;Benlloch, S;Felipo, V;Montoliu, C;
International journal of molecular sciences
Species: Human
Sample Types: Plasma
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Impact of the MCP-1-2518A>G polymorphism on COVID-19 severity in the Iranian population: A case-control study
Authors: Mohammadi, NG;Namaki, S;Hashemi, SM;Salehi, M;Ghaffarpour, S;Ghazanfari, T;
International immunopharmacology
Species: Human
Sample Types: Serum
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Mast cell tolerance in the skin microenvironment to commensal bacteria is controlled by fibroblasts
Authors: A Di Nardo, YL Chang, S Alimohamma, K Masuda-Kur, Z Wang, K Sriram, PA Insel
Cell Reports, 2023-04-28;42(5):112453.
Species: Human
Sample Types:
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Role of triggering receptor expressed on myeloid cells-1 in the mechanotransduction signaling pathways that link low shear stress with inflammation
Authors: M Liu, AN Panagopoul, UM Oguz, S Samant, CH Vasa, DK Agrawal, YS Chatzizisi
Scientific Reports, 2023-03-21;13(1):4656.
Species: Human
Sample Types: Cell Culture Supernates
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Role of PARP and TRPM2 in VEGF Inhibitor-Induced Vascular Dysfunction
Authors: KB Neves, R Alves-Lope, AC Montezano, RM Touyz
Journal of the American Heart Association, 2023-02-20;0(0):e027769.
Species: Human
Sample Types: Cell Culture Supernates
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3D Matrices for Enhanced Encapsulation and Controlled Release of Anti-Inflammatory Bioactive Compounds in Wound Healing
Authors: R Nicu, DE Ciolacu, AR Petrovici, D Rusu, M Avadanei, AC Mihaila, E Butoi, F Ciolacu
International Journal of Molecular Sciences, 2023-02-20;24(4):.
Species: Human
Sample Types: Cell Culture Supernates
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Co-culture models of endothelial cells, macrophages, and vascular smooth muscle cells for the study of the natural history of atherosclerosis
Authors: M Liu, S Samant, CH Vasa, RM Pedrigi, UM Oguz, S Ryu, T Wei, DR Anderson, DK Agrawal, YS Chatzizisi
PLoS ONE, 2023-01-20;18(1):e0280385.
Species: Human
Sample Types: Cell Culture Supernates
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Mammalian Glycosylation Patterns Protect Citrullinated Chemokine MCP-1/CCL2 from Partial Degradation
Authors: O Korchynsky, K Yoshida, N Korchynska, J Czarnik-Kw, PP Tak, GJM Pruijn, T Isozaki, JH Ruth, PL Campbell, MA Amin, AE Koch
International Journal of Molecular Sciences, 2023-01-18;24(3):.
Species: Human
Sample Types: Cell Lysates
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Pyruvate kinase M2 mediates IL-17 signaling in keratinocytes driving psoriatic skin inflammation
Authors: FP Veras, GA Publio, BM Melo, DS Prado, T Norbiato, NT Cecilio, C Hiroki, LEA Damasceno, R Jung, JE Toller-Kaw, TV Martins, SF Assunção, D Lima, MG Alves, GV Vieira, LA Tavares, ALR Alves-Reze, SH Karbach, HI Nakaya, TM Cunha, CS Souza, FQ Cunha, KU Sales, A Waisman, JC Alves-Filh
Cell Reports, 2022-12-27;41(13):111897.
Species: Human
Sample Types: Cell Culture Supernates
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Asprosin Exerts Pro-Inflammatory Effects in THP-1 Macrophages Mediated via the Toll-like Receptor 4 (TLR4) Pathway
Authors: K Shabir, S Gharanei, S Orton, V Patel, P Chauhan, E Karteris, HS Randeva, JE Brown, I Kyrou
International Journal of Molecular Sciences, 2022-12-23;24(1):.
Species: Human
Sample Types: Cell Culture Supernates
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Blood orange juice intake modulates plasma and PBMC microRNA expression in overweight and insulin resistance women: impact on MAPK and NFkappaB signaling pathways
Authors: VC Capetini, BJ Quintanilh, DC de Oliveir, AH Nishioka, LA de Matos, LRP Ferreira, FM Ferreira, GR Sampaio, NMA Hassimotto, FM Lajolo, RA Fock, MM Rogero
The Journal of nutritional biochemistry, 2022-11-26;0(0):109240.
Species: Human
Sample Types: Plasma
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Involvement of glycoinositolphospholipid from Trypanosoma cruzi and macrophage migration inhibitory factor in proinflammatory mechanisms promoting cardiovascular injury mechanisms promoting cardiovascular inflammation tThe combined action of glycoinositolphospholipid from Trypanosoma cruzi and macrophage migration inhibitory factor increases proinflammatory mediator production by cardiomyocytes and vascular endothelial cells
Authors: CS Rigazio, N Mariz-Pont, EP Caballero, FN Penas, NB Goren, MH Santamaría, RS Corral
Microbial pathogenesis, 2022-11-12;0(0):105881.
Species: Human
Sample Types: Cell Culture Supernates
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Exercise training-induced changes in immunometabolic markers in youth badminton athletes
Authors: FE Rossi, AJ Maldonado, JM Cholewa, SLG Ribeiro, CA de Araújo, C Figueiredo, T Reichel, K Krüger, FS Lira, LG Minuzzi
Scientific Reports, 2022-09-15;12(1):15539.
Species: Human
Sample Types: Plasma
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Interleukin 38 alleviates aortic valve calcification by inhibition of NLRP3
Authors: E The, DM de Graaf, Y Zhai, Q Yao, L Ao, DA Fullerton, CA Dinarello, X Meng
Proceedings of the National Academy of Sciences of the United States of America, 2022-08-29;119(36):e2202577119.
Species: Human
Sample Types: Cell Culture Supernates
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Circulating Monocyte Chemoattractant Protein-1 in Patients with Cardiogenic Shock Complicating Acute Myocardial Infarction Treated with Mild Hypothermia: A Biomarker Substudy of SHOCK-COOL Trial
Authors: W Cheng, G Fuernau, S Desch, A Freund, HJ Feistritze, J Pöss, P Buettner, H Thiele
Journal of cardiovascular development and disease, 2022-08-20;9(8):.
Species: Human
Sample Types: Serum
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Pro-cancerogenic effects of spontaneous and drug-induced senescence of ovarian cancer cells in vitro and in vivo: a comparative analysis
Authors: S Rutecki, P Szulc, M Paku?a, P Uruski, A Radziemski, E Naumowicz, R Moszy?ski, A Tykarski, J Miku?a-Pie, K Ksi??ek
Journal of ovarian research, 2022-07-26;15(1):87.
Species: Human
Sample Types: Cell Culture Supernates
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Di-(2-ethylhexyl) phthalate substitutes accelerate human adipogenesis through PPARgamma activation and cause oxidative stress and impaired metabolic homeostasis in mature adipocytes
Authors: A Schaffert, I Karkossa, E Ueberham, R Schlichtin, K Walter, J Arnold, M Blüher, JT Heiker, J Lehmann, M Wabitsch, BI Escher, M von Bergen, K Schubert
Environment international, 2022-05-06;164(0):107279.
Species: Human
Sample Types: Cell Culture Supernates
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Anti-Inflammatory and Anti-Fibrotic Effect of Immortalized Mesenchymal-Stem-Cell-Derived Conditioned Medium on Human Lung Myofibroblasts and Epithelial Cells
Authors: E Filidou, L Kandilogia, G Tarapatzi, M Spathakis, P Steiropoul, D Mikroulis, K Arvanitidi, V Paspaliari, G Kolios
International Journal of Molecular Sciences, 2022-04-20;23(9):.
Species: Human
Sample Types: Cell Culture Supernates
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Discovery of a novel GRPR antagonist for protection against cisplatin-induced acute kidney injury
Authors: MJ Yu, C Li, SS Deng, XM Meng, RS Yao
Bioorganic chemistry, 2022-04-06;124(0):105794.
Species: Human
Sample Types: Cell Culture Supernates
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Transcriptional and DNA Methylation Signatures of Subcutaneous Adipose Tissue and Adipose-Derived Stem Cells in PCOS Women
Authors: A Divoux, E Erdos, K Whytock, TF Osborne, SR Smith
Cells, 2022-03-01;11(5):.
Species: Human
Sample Types: Cell Culture Supernates
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Echinococcus multilocularis specific antibody, systemic cytokine, and chemokine levels, as well as antigen-specific cellular responses in patients with progressive, stable, and cured alveolar echinococcosis: A 10-year follow-up
Authors: B Grüner, L Peters, A Hillenbran, P Vo beta berg, J Schweiker, EG Rollmann, LH Rodriguez, J Blumhardt, S Burkert, P Kern, C Köhler, PT Soboslay
PloS Neglected Tropical Diseases, 2022-02-02;16(2):e0010099.
Species: Human
Sample Types: Cell Culture Supernates
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Kidney injury biomarkers and parasitic loads of Schistosoma mansoni in a highly endemic area in northeastern Brazil
Authors: RLF Galvão, GC Meneses, MCC Pinheiro, AMC Martins, EF Daher, FSM Bezerra
Acta tropica, 2022-01-14;0(0):106311.
Species: Human
Sample Types: Urine
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Chemerin Overexpression in the Liver Protects against Inflammation in Experimental Non-Alcoholic Steatohepatitis
Authors: R Pohl, S Feder, EM Haberl, L Rein-Fisch, TS Weiss, M Spirk, A Bruckmann, N McMullen, CJ Sinal, C Buechler
Biomedicines, 2022-01-07;10(1):.
Species: Human
Sample Types: Cell Culture Supernates
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A green-lipped mussel reduces pain behavior and chondrocyte inflammation and attenuated experimental osteoarthritis progression
Authors: J Jhun, HS Na, KH Cho, J Kim, YM Moon, SY Lee, JS Lee, AR Lee, SJ Kim, ML Cho, SH Park
PLoS ONE, 2021-12-02;16(12):e0259130.
Species: Human
Sample Types: Cell Culture Supernates
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Systematic investigation of cytokine signaling activity at the tissue and single-cell levels
Authors: P Jiang, Y Zhang, B Ru, Y Yang, T Vu, R Paul, A Mirza, G Altan-Bonn, L Liu, E Ruppin, L Wakefield, KW Wucherpfen
Nature methods, 2021-09-30;18(10):1181-1191.
Species: Human
Sample Types: Cell Culture Supernates
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Identification of soluble tissue-derived biomarkers from human thyroid tissue explants maintained on a microfluidic device
Authors: A Riley, H Jones, J England, D Kuvshinov, V Green, J Greenman
Oncology Letters, 2021-09-13;22(5):780.
Species: Human
Sample Types: Cell Culture Supernates
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Development of a Human Intestinal Organoid Model for In Vitro Studies on Gut Inflammation and Fibrosis
Authors: L Kandilogia, E Filidou, I Drygiannak, G Tarapatzi, S Didaskalou, M Koffa, K Arvanitidi, G Bamias, V Valatas, V Paspaliari, G Kolios
Stem Cells International, 2021-07-27;2021(0):9929461.
Species: Human
Sample Types: Cell Culture Supernates
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Nobiletin Decreases Inflammatory Mediator Expression in Tumor Necrosis Factor-Stimulated Human Periodontal Ligament Cells
Authors: Y Hosokawa, I Hosokawa, K Ozaki
Mediators of Inflammation, 2021-07-10;2021(0):5535844.
Species: Human
Sample Types: Cell Culture Supernates
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Alternatives for the worse: Molecular insights into adverse effects of bisphenol a and substitutes during human adipocyte differentiation
Authors: A Schaffert, L Krieg, J Weiner, R Schlichtin, E Ueberham, I Karkossa, M Bauer, K Landgraf, KM Junge, M Wabitsch, J Lehmann, BI Escher, AC Zenclussen, A Körner, M Blüher, JT Heiker, M von Bergen, K Schubert
Environment international, 2021-06-27;156(0):106730.
Species: Human
Sample Types: Cell Culture Supernates
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Identification of monoclonal antibodies against human renal glomerular endothelial cells in lupus nephritis that induce endothelial interferon-alpha production
Authors: YC Hu, IJ Tsai, HY Hsu, BL Chiang, YH Yang
Arthritis Research & Therapy, 2021-06-16;23(1):171.
Species: Human
Sample Types: Cell Culture Supernates
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Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution
Authors: LG Lima, S Ham, H Shin, EPZ Chai, ESH Lek, RJ Lobb, AF Müller, S Mathivanan, B Yeo, Y Choi, BS Parker, A Möller
Nature Communications, 2021-06-10;12(1):3543.
Species: Human
Sample Types: Exosomes
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Beneficial Effects of Mineralocorticoid Receptor Pathway Blockade against Endothelial Inflammation Induced by SARS-CoV-2 Spike Protein
Authors: E Jover, L Matilla, M Garaikoetx, A Fernández-, P Muntendam, F Jaisser, P Rossignol, N López-Andr
Biomedicines, 2021-06-03;9(6):.
Species: Human
Sample Types: Cell Culture Supernates
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Thrombin impairs the angiogenic activity of extravillous trophoblast cells via monocyte chemotactic protein-1 (MCP-1): A possible link with preeclampsia
Authors: D Brünnert, V Kumar, V Kaushik, J Ehrhardt, KR Chahar, PK Sharma, M Zygmunt, P Goyal
Reproductive biology, 2021-05-28;21(3):100516.
Species: Human
Sample Types: Cell Culture Supernates
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Inflammatory responses to metal oxide ceramic nanopowders
Authors: S Jamieson, A Mawdesley, D Deehan, J Kirby, J Holland, A Tyson-Capp
Scientific Reports, 2021-05-18;11(1):10531.
Species: Human
Sample Types: Cell Culture Supernates
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Melanoma Associated Chitinase 3-Like 1 Promoted Endothelial Cell Activation and Immune Cell Recruitment
Authors: G Ramos-Espi, Y Wang, JM Brandner, SW Schneider, C Gorzelanny
International Journal of Molecular Sciences, 2021-04-10;22(8):.
Species: Human
Sample Types: Cell Culture Supernates
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Adipocytokines in Untreated Newly Diagnosed Rheumatoid Arthritis: Association with Circulating Chemokines and Markers of Inflammation
Authors: GK Vasileiadi, AC Lundell, Y Zhang, K Andersson, I Gjertsson, A Rudin, C Maglio
Biomolecules, 2021-02-21;11(2):.
Species: Human
Sample Types: Plasma
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The Polymethoxy Flavonoid Sudachitin Inhibits Interleukin-1&beta-Induced Inflammatory Mediator Production in Human Periodontal Ligament Cells
Authors: Y Hosokawa, I Hosokawa, K Ozaki, T Matsuo
BioMed Research International, 2021-01-28;2021(0):8826586.
Species: Human
Sample Types: Cell Culture Supernates
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Polyphenol-rich extract and fractions of Terminalia phaeocarpa Eichler possess hypoglycemic effect, reduce the release of cytokines, and inhibit lipase, &alpha-glucosidase, and &alpha-amilase enzymes
Authors: JHS Gomes, UC Mbiakop, RL Oliveira, JR Stehmann, RM Pádua, SF Cortes, FC Braga
Journal of ethnopharmacology, 2021-01-27;271(0):113847.
Species: Human
Sample Types: Cell Culture Supernates
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Exploring the Role of C-C Motif Chemokine Ligand-2 Single Nucleotide Polymorphism in Pulmonary Tuberculosis: A Genetic Association Study from North India
Authors: SK Biswas, M Mittal, E Sinha, V Singh, N Arela, B Bajaj, PK Tiwari, VM Katoch, KK Mohanty
Journal of Immunology Research, 2020-12-16;2020(0):1019639.
Species: Human
Sample Types: Serum
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Resveratrol treatment reduces expression of MCP-1, IL-6, IL-8 and RANTES in endometriotic stromal cells
Authors: R Kolahdouz-, F Shidfar, S Khodaverdi, T Arablou, S Heidari, N Rashidi, AA Delbandi
Journal of Cellular and Molecular Medicine, 2020-12-15;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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Clinical Relevance of the Anti-inflammatory Effects of Roflumilast on Human Bronchus: Potentiation by a Long-Acting Beta-2-Agonist
Authors: H Salvator, A Buenestado, M Brollo, E Naline, T Victoni, E Longchamp, H Tenor, S Grassin-De, P Devillier
Frontiers in Pharmacology, 2020-12-08;11(0):598702.
Species: Human
Sample Types: Cell Culture Supernates
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Widespread regulation of gene expression by glucocorticoids in chondrocytes from patients with osteoarthritis as determined by RNA-Seq
Authors: A Pemmari, T Leppänen, M Hämäläinen, T Moilanen, K Vuolteenah, E Moilanen
Arthritis Res Ther, 2020-11-17;22(1):271.
Species: Human
Sample Types: Cell Culture Supernates
-
Paracrine signalling by cardiac calcitonin controls atrial fibrogenesis and arrhythmia
Authors: LM Moreira, A Takawale, M Hulsurkar, DA Menassa, A Antanavici, SK Lahiri, N Mehta, N Evans, C Psarros, P Robinson, AJ Sparrow, MA Gillis, N Ashley, P Naud, J Barallobre, K Theofilato, A Lee, M Norris, MV Clarke, PK Russell, B Casadei, S Bhattachar, JD Zajac, RA Davey, M Sirois, A Mead, A Simmons, M Mayr, R Sayeed, G Krasopoulo, C Redwood, KM Channon, JC Tardif, XHT Wehrens, S Nattel, S Reilly
Nature, 2020-11-04;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
-
An observational study of innate immune responses in patients with acute appendicitis
Authors: T Peeters, S Martens, V D'Onofrio, MHT Stappers, JCH van der Hi, B Houben, R Achten, LAB Joosten, IC Gyssens
Sci Rep, 2020-10-15;10(1):17352.
Species: Human
Sample Types: Serum
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Gene expression network analyses during infection with virulent and avirulent Trypanosoma cruzi�strains unveil a role for fibroblasts in neutrophil recruitment and activation
Authors: AER Oliveira, MCA Pereira, AT Belew, LRP Ferreira, LMN Pereira, EGA Neves, MDCP Nunes, BA Burleigh, WO Dutra, NM El-Sayed, RT Gazzinelli, SMR Teixeira
PLoS Pathog., 2020-08-18;16(8):e1008781.
Species: Human
Sample Types: Cell Culture Supernates
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TASL is the SLC15A4-associated adaptor for IRF5 activation by TLR7-9
Authors: LX Heinz, J Lee, U Kapoor, F Kartnig, V Sedlyarov, K Papakostas, A César-Razq, P Essletzbic, U Goldmann, A Stefanovic, JW Bigenzahn, S Scorzoni, MD Pizzagalli, A Bensimon, AC Müller, FJ King, J Li, E Girardi, ML Mbow, CE Whitehurst, M Rebsamen, G Superti-Fu
Nature, 2020-05-13;581(7808):316-322.
Species: Human
Sample Types: Cell Culture Supernates
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Antifibrotic Effects of a Barbituric Acid Derivative on Liver Fibrosis by Blocking the NF-&kappaB Signaling Pathway in Hepatic Stellate Cells
Authors: YH Wang, FM Suk, CL Liu, TL Chen, YC Twu, MH Hsu, YJ Liao
Front Pharmacol, 2020-03-31;11(0):388.
Species: Human
Sample Types: Cell Culture Supernates
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Anti-CD3 Antibody Treatment Reduces Scar Formation in a Rat Model of Myocardial Infarction
Authors: B Wernly, V Paar, A Aigner, PM Pilz, BK Podesser, M Förster, C Jung, JP Hofbauer, B Tockner, M Wimmer, T Kraus, LJ Motloch, M Hackl, UC Hoppe, A Kiss, M Lichtenaue
Cells, 2020-01-25;9(2):.
Species: Human
Sample Types: Cell Culture Supernates
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Neutrophil microvesicles drive atherosclerosis by delivering miR-155 to atheroprone endothelium
Authors: I Gomez, B Ward, C Souilhol, C Recarti, M Ariaans, J Johnston, A Burnett, M Mahmoud, LA Luong, L West, M Long, S Parry, R Woods, C Hulston, B Benedikter, C Niespolo, R Bazaz, S Francis, E Kiss-Toth, M van Zandvo, A Schober, P Hellewell, PC Evans, V Ridger
Nat Commun, 2020-01-10;11(1):214.
Species: Human
Sample Types: Cell Culture Supernates
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Replication of Genome-Wide Association Analysis Identifies New Susceptibility Loci at Long Noncoding RNA Regions for Vogt-Koyanagi-Harada Disease
Authors: J Qi, L Du, J Deng, Y Qin, G Su, S Hou, M Lv, Q Zhang, A Kijlstra, P Yang
Invest. Ophthalmol. Vis. Sci., 2019-11-01;60(14):4820-4829.
Species: Human
Sample Types: Cell Culture Supernates
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Type 1 diabetic mellitus patients with increased atherosclerosis risk display decreased CDKN2A/2B/2BAS gene expression in leukocytes
Authors: S Martínez-H, V Sánchez-Ga, A Herrero-Ce, Á Vinué, JT Real, JF Ascaso, DJ Burks, H González-N
J Transl Med, 2019-07-12;17(1):222.
Species: Human
Sample Types: Plasma
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Translational regulation contributes to the secretory response of chondrocytic cells following exposure to interleukin-1 β
Authors: BT McDermott, MJ Peffers, B McDonagh, SR Tew
J. Biol. Chem., 2019-07-12;294(35):13027-13039.
Species: Human
Sample Types: Cell Culture Supernates
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Melatonin Modulates the Microenvironment of Glioblastoma Multiforme by Targeting Sirtuin 1
Authors: SW Lai, YS Liu, DY Lu, CF Tsai
Nutrients, 2019-06-14;11(6):.
Species: Human
Sample Types: Cell Culture Supernates
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Angiogenic and Arthritogenic Properties of the Soluble Form of CD13
Authors: Y Du, C Lu, RL Morgan, WA Stinson, PL Campbell, E Cealey, W Fu, NJ Lepore, JL Hervoso, H Cui, AG Urquhart, JN Lawton, KC Chung, DA Fox, MA Amin
J. Immunol., 2019-06-12;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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The human glomerular endothelial cells are potent pro-inflammatory contributors in an in vitro model of lupus nephritis
Authors: P Dimou, RD Wright, KL Budge, A Midgley, SC Satchell, M Peak, MW Beresford
Sci Rep, 2019-06-06;9(1):8348.
Species: Human
Sample Types: Cell Culture Supernates
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A Unique Pattern of Mesothelial-Mesenchymal Transition Induced in the Normal Peritoneal Mesothelium by High-Grade Serous Ovarian Cancer
Authors: M Paku?a, P Uruski, A Niklas, A Wo?niak, D Szpurek, A Tykarski, J Miku?a-Pie, K Ksi??ek
Cancers (Basel), 2019-05-13;11(5):.
Species: Human
Sample Types: Cell Culture Supernates
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Calcineurin inhibitors reduce NFAT-dependent expression of antifungal pentraxin-3 by human monocytes
Authors: K Bendí?ková, F Tidu, M De Zuani, MH Kohoutková, I Andrej?ino, A Pompeiano, S B?lášková, G Forte, T Zelante, J Fri?
J. Leukoc. Biol., 2019-04-01;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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Probiotic Lactobacillus rhamnosus GR-1 is a unique prophylactic agent that suppresses infection-induced myometrial cell responses
Authors: B Kim, O Shynlova, S Lye
Sci Rep, 2019-03-18;9(1):4698.
Species: Human
Sample Types: Cell Culture Supernates
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Circulating Monocyte and Lymphocyte Populations in Healthy First-Degree Relatives of Type 2 Diabetic Patients at Fasting and during Short-Term Hyperinsulinemia
Authors: M Šiklová, E Krauzová, B Svobodová, J Kra?merová, M Št?pán, M Koc, V Štich, L Rossmeislo
Mediators Inflamm., 2019-03-11;2019(0):1491083.
Species: Human
Sample Types: Plasma
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Toll-like receptor 3 regulates Zika virus infection and associated host inflammatory response in primary human astrocytes
Authors: CR Ojha, M Rodriguez, MKM Karuppan, J Lapierre, F Kashanchi, N El-Hage
PLoS ONE, 2019-02-08;14(2):e0208543.
Species: Human
Sample Types: Cell Culture Supernates
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Bone Marrow Mesenchymal Stromal Cell-Derived Periostin Promotes B-ALL Progression by Modulating CCL2 in Leukemia Cells
Authors: Z Ma, X Zhao, M Deng, Z Huang, J Wang, Y Wu, D Cui, Y Liu, R Liu, G Ouyang
Cell Rep, 2019-02-05;26(6):1533-1543.e4.
Species: Human
Sample Types: Plasma
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PKC? facilitates lymphatic metastatic spread of prostate cancer cells in a mice xenograft model
Authors: G Zang, Y Mu, L Gao, A Bergh, M Landström
Oncogene, 2019-01-31;38(22):4215-4231.
Species: Human
Sample Types: Cell Culture Supernates
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A Transient Pseudosenescent Secretome Promotes Tumor Growth after Antiangiogenic Therapy Withdrawal
Authors: M Mastri, A Tracz, CR Lee, M Dolan, K Attwood, JG Christense, S Liu, JML Ebos
Cell Rep, 2018-12-26;25(13):3706-3720.e8.
Species: Human
Sample Types: Cell Culture Supernates
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Combining Calcium Phosphates with Polysaccharides: A Bone-Inspired Material Modulating Monocyte/Macrophage Early Inflammatory Response
Authors: H Rammal, C Bour, M Dubus, L Entz, L Aubert, SC Gangloff, S Audonnet, NB Bercu, F Boulmedais, C Mauprivez, H Kerdjoudj
Int J Mol Sci, 2018-11-03;19(11):.
Species: Human
Sample Types: Cell Culture Supernates
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Placental Ras Regulates Inflammation Associated with Maternal Obesity
Authors: S Liong, G Barker, M Lappas
Mediators Inflamm., 2018-10-08;2018(0):3645386.
Species: Human
Sample Types: Cell Culture Supernates
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Inhibition of Proteasome Activity Upregulates IL-6 Expression in RPE Cells through the Activation of P38 MAPKs
Authors: T Qin, S Gao
J Ophthalmol, 2018-07-12;2018(0):5392432.
Species: Human
Sample Types: Cell Culture Supernates
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The immunoproteasome inhibitor ONX-0914 regulates inflammation and expression of contraction associated proteins in myometrium
Authors: S Liong, R Lim, C Nguyen-Ngo, G Barker, HC Parkington, M Lappas
Eur. J. Immunol., 2018-06-28;0(0):.
Species: Human
Sample Types: Tissue Homogenates
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Genetic Polymorphism at CCL5 Is Associated With Protection in Chagas' Heart Disease: Antagonistic Participation of CCR1+ and CCR5+ Cells in Chronic Chagasic Cardiomyopathy.
Authors: Angelica Martins Batista, Lucia Elena Alvarado-, Silvia Marinho Alves, Gloria Melo, Isabela Resende Pereira, Leonardo Alexandre de Sou Ruivo, Andrea Alice Da Silva, Daniel Gibaldi, Thayse Do E S Protásio Da Silva, Virginia Maria Barros De Lorena, Adriene Siqueira De Melo, Ana Karine De Araújo, Michelle Da Silva Barros, Vláudia Maria Assis Costa, Cynthia C Cardoso, Antonio G Pacheco, Cristina Carrazzon, Wilson Oliveira, Milton Ozório Moraes, Joseli Lannes-Vi
Frontiers in Immunology, 2018-04-11;0(0):1664-3224.
Species: Human
Sample Types: Serum
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Analysis of the association between Fc receptor family gene polymorphisms and ocular Beh�et's disease in Han Chinese
Authors: D Zhang, J Qin, L Li, G Su, G Huang, Q Cao, A Kijlstra, P Yang
Sci Rep, 2018-03-19;8(1):4850.
Species: Human
Sample Types: Cell Culture Supernates
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Association of Long Noncoding RNAs Polymorphisms With Ankylosing Spondylitis, Vogt-Koyanagi-Harada Disease, and Behcet's Disease
Authors: Y Yue, J Zhang, L Yang, S Liu, J Qi, Q Cao, C Zhou, Y Wang, A Kijlstra, P Yang, S Hou
Invest. Ophthalmol. Vis. Sci., 2018-02-01;59(2):1158-1166.
Species: Human
Sample Types: Cell Culture Supernates
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Site-specific and endothelial-mediated dysfunction of the alveolar-capillary barrier in response to lipopolysaccharides
Authors: H Janga, L Cassidy, F Wang, D Spengler, S Oestern-Fi, MF Krause, A Seekamp, A Tholey, S Fuchs
J. Cell. Mol. Med., 2017-12-05;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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Pathogenic role of glycan-specific IgG antibodies in IgA nephropathy
Authors: YF Zhao, L Zhu, LJ Liu, SF Shi, JC Lv, H Zhang
BMC Nephrol, 2017-09-29;18(1):301.
Species: Human
Sample Types: Cell Culture Supernates
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The Proangiogenic Capabilities of Malignant Ascites Generated by Aggressive Ovarian Tumors
Authors: J Miku?a-Pie, P Uruski, S Szubert, K Maksin, R Moszy?ski, D Szpurek, A Wo?niak, S Sajdak, A Tykarski, K Ksi??ek
Biomed Res Int, 2017-09-20;2017(0):2592496.
Species: Human
Sample Types: Cell Culture Supernates
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Alteration of Endothelin 1, MCP-1 and Chromogranin A in patients with atrial fibrillation undergoing pulmonary vein isolation
Authors: K Lackermair, S Clauss, T Voigt, I Klier, C Summo, B Hildebrand, T Nickel, HL Estner, S Kääb, R Wakili, U Wilbert-La
PLoS ONE, 2017-09-08;12(9):e0184337.
Species: Human
Sample Types: Plasma
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Minocycline modulates NF?B phosphorylation and enhances antimicrobial activity against Staphylococcus aureus in mesenchymal stromal/stem cells
Authors: AD Guerra, WE Rose, P Hematti, WJ Kao
Stem Cell Res Ther, 2017-07-21;8(1):171.
Species: Human
Sample Types: Cell Culture Supernates
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The Role of Shed PrP(c) in the Neuropathogenesis of HIV Infection
Authors: BW Megra, EA Eugenin, JW Berman
J. Immunol., 2017-05-22;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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Excess atherosclerosis in systemic lupus erythematosus,-A matter of renal involvement: Case control study of 281 SLE patients and 281 individually matched population controls
Authors: JT Gustafsson, M Herlitz Li, I Gunnarsson, S Pettersson, K Elvin, J Öhrvik, A Larsson, K Jensen-Urs, E Svenungsso
PLoS ONE, 2017-04-17;12(4):e0174572.
Species: Human
Sample Types: Tissue Homogenates
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Presence of IL-17 in synovial fluid identifies a potential inflammatory osteoarthritic phenotype
Authors: SJ Snelling, S Bas, GJ Puskas, SG Dakin, D Suva, A Finckh, C Gabay, P Hoffmeyer, AJ Carr, A Lübbeke
PLoS ONE, 2017-04-11;12(4):e0175109.
Species: Human
Sample Types: Serum
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Serum retinol-binding protein-induced endothelial inflammation is mediated through the activation of toll-like receptor 4
Authors: M Du, A Martin, F Hays, J Johnson, RA Farjo, KM Farjo
Mol. Vis., 2017-03-31;23(0):185-197.
Species: Human
Sample Types: Cell Culture Supernates
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Caspase-8 Acts in a Non-enzymatic Role as a Scaffold for Assembly of a Pro-inflammatory FADDosome Complex upon TRAIL Stimulation
Authors: CM Henry, SJ Martin
Mol. Cell, 2017-02-16;65(4):715-729.e5.
Species: Human
Sample Types: Cell Culture Supernates
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The TRAIL-Induced Cancer Secretome Promotes a Tumor-Supportive Immune Microenvironment via CCR2
Authors: T Hartwig, A Montinaro, S von Karste, A Sevko, S Surinova, A Chakravart, L Taraborrel, P Draber, E Lafont, F Arce Varga, MA El-Bahrawy, SA Quezada, H Walczak
Mol. Cell, 2017-02-16;65(4):730-742.e5.
Species: Human
Sample Types: Cell Culture Supernates
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Human Lymph Node-Derived Fibroblastic and Double-Negative Reticular Cells Alter Their Chemokines and Cytokines Expression Profile Following Inflammatory Stimuli
Authors: P Severino, DT Palomino, H Alvarenga, CB Almeida, DC Pasqualim, A Cury, PR Salvalaggi, AL De Vasconc, MC Andrade, T Aloia, S Bromberg, LV Rizzo, FA Rocha, LC Marti
Front Immunol, 2017-02-14;8(0):141.
Species: Human
Sample Types: Cell Culture Supernates
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DHA-derived oxylipins, neuroprostanes and protectins, differentially and dose-dependently modulate the inflammatory response in human macrophages: putative mechanisms through PPAR activation
Authors: Rémy Bosviel
Free Radic. Biol. Med, 2016-12-14;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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Receptor-Type Protein-Tyrosine Phosphatase ? and Colony Stimulating Factor-1 Receptor in the Intestine: Cellular Expression and Cytokine- and Chemokine Responses by Interleukin-34 and Colony Stimulating Factor-1
PLoS ONE, 2016-11-29;11(11):e0167324.
Species: Human
Sample Types: Cell Culture Supernates
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Modulating endotoxin activity by combinatorial bioengineering of meningococcal lipopolysaccharide
Sci Rep, 2016-11-14;6(0):36575.
Species: Human
Sample Types: Cell Culture Supernates
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Identification of Iguratimod as an Inhibitor of Macrophage Migration Inhibitory Factor (MIF) with Steroid-Sparing Potential
Authors: Yousef Al-Abed
J. Biol. Chem., 2016-10-28;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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In Vitro Inhibition of NFAT5-Mediated Induction of CCL2 in Hyperosmotic Conditions by Cyclosporine and Dexamethasone on Human HeLa-Modified Conjunctiva-Derived Cells
PLoS ONE, 2016-08-03;11(8):e0159983.
Species: Human
Sample Types: Cell Culture Supernates
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Graphene-augmented nanofiber scaffolds demonstrate new features in cells behaviour
Sci Rep, 2016-07-22;6(0):30150.
Species: Human
Sample Types: Cell Culture Supernates
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Genetic polymorphisms of cell adhesion molecules in Behcet's disease in a Chinese Han population
Authors: M Zheng, L Zhang, H Yu, J Hu, Q Cao, G Huang, Y Huang, G Yuan, A Kijlstra, P Yang
Sci Rep, 2016-04-25;6(0):24974.
Species: Human
Sample Types: Cell Culture Supernates
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Unfractionated Heparin Selectively Modulates the Expression of CXCL8, CCL2 and CCL5 in Endometrial Carcinoma Cells
Authors: A Doster, U Schwarzig, M Zygmunt, J Rom, F Schütz, H Fluhr
Anticancer Res, 2016-04-01;36(4):1535-44.
Species: Human
Sample Types: Cell Culture Supernates
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Increased occurrence of protein kinase CK2 in astrocytes in Alzheimer's disease pathology.
Authors: Rosenberger A, Morrema T, Gerritsen W, van Haastert E, Snkhchyan H, Hilhorst R, Rozemuller A, Scheltens P, van der Vies S, Hoozemans J
J Neuroinflammation, 2016-01-06;13(1):4.
Species: Human
Sample Types: Cell Culture Supernates
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Characterization of In Vitro Engineered Human Adipose Tissues: Relevant Adipokine Secretion and Impact of TNF-alpha.
Authors: Aubin K, Safoine M, Proulx M, Audet-Casgrain M, Côté J, Tetu F, Roy A, Fradette J
PLoS ONE, 2015-09-14;10(9):e0137612.
Species: Human
Sample Types: Cell Culture Supernates
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Inflammatory and Angiogenic Factors at Mid-Pregnancy Are Associated with Spontaneous Preterm Birth in a Cohort of Tanzanian Women.
Authors: McDonald C, Darling A, Conroy A, Tran V, Cabrera A, Liles W, Wang M, Aboud S, Urassa W, Fawzi W, Kain K
PLoS ONE, 2015-08-06;10(8):e0134619.
Species: Human
Sample Types: Plasma
-
Amyloid precursor protein mediated changes in intestinal epithelial phenotype in vitro.
Authors: Puig K, Manocha G, Combs C
PLoS ONE, 2015-03-05;10(3):e0119534.
Species: Human
Sample Types: Cell Culture Supernates
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Macrophage activation in acute exacerbation of idiopathic pulmonary fibrosis.
Authors: Schupp J, Binder H, Jager B, Cillis G, Zissel G, Muller-Quernheim J, Prasse A
PLoS ONE, 2015-01-15;10(1):e0116775.
Species: Human
Sample Types: BALF
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Integrin alphaDbeta2 (CD11d/CD18) is expressed by human circulating and tissue myeloid leukocytes and mediates inflammatory signaling.
Authors: Miyazaki Y, Vieira-de-Abreu A, Harris E, Shah A, Weyrich A, Castro-Faria-Neto H, Zimmerman G
PLoS ONE, 2014-11-21;9(11):e112770.
Species: Human
Sample Types: Cell Culture Supernates
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Cessation of CCL2 inhibition accelerates breast cancer metastasis by promoting angiogenesis.
Authors: Bonapace L, Coissieux M, Wyckoff J, Mertz K, Varga Z, Junt T, Bentires-Alj M
Nature, 2014-10-22;515(7525):130-3.
Species: Human
Sample Types: Cell Culture Supernates
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A functional variant of PTPN22 confers risk for Vogt-Koyanagi-Harada syndrome but not for ankylosing spondylitis.
Authors: Zhang Q, Qi J, Hou S, DU L, Yu H, Cao Q, Zhou Y, Liao D, Kijlstra A, Yang P
PLoS ONE, 2014-05-09;9(5):e96943.
Species: Human
Sample Types: Cell Culture Supernates
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Human adipose tissue macrophages are enhanced but changed to an anti-inflammatory profile in obesity.
Authors: Fjeldborg K, Pedersen S, Moller H, Christiansen T, Bennetzen M, Richelsen B
J Immunol Res, 2014-03-11;2014(0):309548.
Species: Human
Sample Types: Serum
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Neisseria gonorrhoeae modulates iron-limiting innate immune defenses in macrophages.
Authors: Zughaier, Susu M, Kandler, Justin L, Shafer, William
PLoS ONE, 2014-01-28;9(1):e87688.
Species: Human
Sample Types: Cell Culture Supernates
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CD137 ligand signalling induces differentiation of primary acute myeloid leukaemia cells.
Authors: Cheng K, Wong S, Linn Y, Ho L, Chng W, Schwarz H
Br J Haematol, 2014-01-15;165(1):134-44.
Species: Human
Sample Types: Cell Culture Supernates
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Lung collagens perpetuate pulmonary fibrosis via CD204 and M2 macrophage activation.
Authors: Stahl M, Schupp J, Jager B, Schmid M, Zissel G, Muller-Quernheim J, Prasse A
PLoS ONE, 2013-11-20;8(11):e81382.
Species: Human
Sample Types: Cell Culture Supernates
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The macrophage-specific serum marker, soluble CD163, is increased in obesity and reduced after dietary-induced weight loss.
Authors: Fjeldborg K, Christiansen T, Bennetzen M, J Moller H, Pedersen S, Richelsen B
Obesity (Silver Spring), 2013-07-02;21(12):2437-43.
Species: Human
Sample Types: Serum
-
1,25-dihydroxyvitamin D3 protects against macrophage-induced activation of NFkappaB and MAPK signalling and chemokine release in human adipocytes.
Authors: Ding C, Wilding J, Bing C
PLoS ONE, 2013-04-24;8(4):e61707.
Species: Human
Sample Types: Cell Culture Supernates
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Human cytomegalovirus induces a biphasic inflammatory response in primary endothelial cells.
Authors: Jeffery H, Soderberg-Naucler C, Butler L
J Virol, 2013-03-27;87(11):6530-5.
Species: Human
Sample Types: Cell Culture Supernates
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Effects of high-dose cholecalciferol on serum markers of inflammation and immunity in patients with early chronic kidney disease.
Authors: Alvarez J, Zughaier S, Law J, Hao L, Wasse H, Ziegler T, Tangpricha V
Eur J Clin Nutr, 2013-01-30;67(3):264-9.
Species: Human
Sample Types: Serum
-
Characterization and function of the human macrophage dopaminergic system: implications for CNS disease and drug abuse.
J Neuroinflammation, 2012-08-18;9(0):203.
Species: Human
Sample Types: Cell Culture Supernates
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Urine VCAM-1 as a marker of renal pathology activity index in lupus nephritis.
Authors: Singh S, Wu T
Oncogene, 2012-07-13;14(4):R164.
Species: Human
Sample Types: Urine
-
Plasma levels of procalcitonin and eight additional inflammatory molecules in febrile neutropenic patients.
Authors: Neuenschwander LC, Bittencourt H, Ribeiro AF, Teixeira AL, Teixeira MM, Teixeira JC, Nobre V
Clinics (Sao Paulo), 2011-01-01;66(10):1699-705.
Species: Human
Sample Types: Plasma
-
Focal increases of fetal macrophages in placentas from pregnancies with histological chorioamnionitis: potential role of fibroblast monocyte chemotactic protein-1.
Authors: Toti P, Arcuri F, Tang Z, Schatz F, Zambrano E, Mor G, Niven-Fairchild T, Abrahams VM, Krikun G, Lockwood CJ, Guller S
Am. J. Reprod. Immunol., 2010-11-19;65(5):470-9.
Species: Human
Sample Types: Cell Culture Supernates
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Activation of platelets by Aspergillus fumigatus and potential role of platelets in the immunopathogenesis of Aspergillosis.
Authors: Rodland EK, Ueland T, Pedersen TM, Halvorsen B, Muller F, Aukrust P, Froland SS
Infect. Immun., 2009-12-14;78(3):1269-75.
Species: Human
Sample Types: Cell Culture Supernates
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Genetic variants of CC chemokine genes in experimental autoimmune encephalomyelitis, multiple sclerosis and rheumatoid arthritis.
Authors: Ockinger J, Stridh P, Beyeen AD, Lundmark F, Seddighzadeh M, Oturai A, Sorensen PS, Lorentzen AR, Celius EG, Leppa V, Koivisto K, Tienari PJ, Alfredsson L, Padyukov L, Hillert J, Kockum I, Jagodic M, Olsson T
Genes Immun., 2009-10-29;11(2):142-54.
Species: Human
Sample Types: CSF
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Oxidized alpha1-antitrypsin stimulates the release of monocyte chemotactic protein-1 from lung epithelial cells: potential role in emphysema.
Authors: Li Z, Alam S, Wang J, Sandstrom CS, Janciauskiene S, Mahadeva R
Am. J. Physiol. Lung Cell Mol. Physiol., 2009-06-12;297(2):L388-400.
Species: Human
Sample Types: Cell Culture Supernates
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Urinary monocyte chemoattractant protein-1 (MCP-1) and connective tissue growth factor (CCN2) as prognostic markers for progression of diabetic nephropathy.
Authors: Tam FW, Riser BL, Meeran K, Rambow J, Pusey CD, Frankel AH
Cytokine, 2009-05-05;47(1):37-42.
Species: Human
Sample Types: Urine
-
Abnormalities in serum chemokine levels in euthymic patients with bipolar disorder.
Authors: Brietzke E, Kauer-Sant'Anna M, Teixeira AL, Kapczinski F
Brain Behav. Immun., 2009-05-04;23(8):1079-82.
Species: Human
Sample Types: Serum
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Carbon dioxide inhalation causes pulmonary inflammation.
Authors: Abolhassani M, Guais A, Chaumet-Riffaud P, Sasco AJ, Schwartz L
Am. J. Physiol. Lung Cell Mol. Physiol., 2009-01-09;296(4):L657-65.
Species: Human
Sample Types: Cell Culture Supernates
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Novel markers of inflammation identified in tumor necrosis factor receptor-associated periodic syndrome (TRAPS) by transcriptomic analysis of effects of TRAPS-associated tumor necrosis factor receptor type I mutations in an endothelial cell line.
Authors: Rebelo SL, Amel-Kashipaz MR, Radford PM, Bainbridge SE, Fiets R, Fang J, McDermott EM, Powell RJ, Todd I, Tighe PJ
Arthritis Rheum., 2009-01-01;60(1):269-80.
Species: Human
Sample Types: Cell Culture Supernates
-
Altered levels of CC chemokines during pulmonary CMV predict BOS and mortality post-lung transplantation.
Authors: Weigt SS, Elashoff RM, Keane MP, Strieter RM, Gomperts BN, Xue YY, Ardehali A, Gregson AL, Kubak B, Fishbein MC, Saggar R, Ross DJ, Lynch JP, Zisman DA, Belperio JA
Am. J. Transplant., 2008-07-01;8(7):1512-22.
Species: Human
Sample Types: BALF
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Expanded-polyglutamine huntingtin protein suppresses the secretion and production of a chemokine (CCL5/RANTES) by astrocytes.
Authors: Chou SY, Weng JY, Lai HL, Liao F, Sun SH, Tu PH, Dickson DW, Chern Y
J. Neurosci., 2008-03-26;28(13):3277-90.
Species: Human
Sample Types: Cell Culture Supernates
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Lipopolysaccharide-squamous cell carcinoma-monocyte interactions induce cancer-supporting factors leading to rapid STAT3 activation.
Authors: Kurago ZB, Lam-ubol A, Stetsenko A
Head Neck Pathol, 2008-03-01;2(1):1-12.
Species: Human
Sample Types: Cell Culture Supernates
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Human epithelial cells establish direct antifungal defense through TLR4-mediated signaling.
Authors: Weindl G, Naglik JR, Kaesler S, Biedermann T, Hube B, Korting HC, Schaller M
J. Clin. Invest., 2007-12-01;117(12):3664-3672.
Species: Human
Sample Types: Cell Culture Supernates
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Increased serum levels of CCL11/eotaxin in schizophrenia.
Authors: Teixeira AL, Reis HJ, Nicolato R, Brito-Melo G, Correa H, Teixeira MM, Romano-Silva MA
Prog. Neuropsychopharmacol. Biol. Psychiatry, 2007-11-23;32(3):710-4.
Species: Human
Sample Types: Serum
-
Elevated urinary VCAM-1, P-selectin, soluble TNF receptor-1, and CXC chemokine ligand 16 in multiple murine lupus strains and human lupus nephritis.
Authors: Wu T, Xie C, Wang HW, Zhou XJ, Schwartz N, Calixto S, Mackay M, Aranow C, Putterman C, Mohan C
J. Immunol., 2007-11-15;179(10):7166-75.
Species: Human
Sample Types: Urine
-
Preadipocyte response and impairment of differentiation in an inflammatory environment.
Authors: Poulain-Godefroy O, Froguel P
Biochem. Biophys. Res. Commun., 2007-03-19;356(3):662-7.
Species: Human
Sample Types: Cell Culture Supernates
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Modification of TLR-induced activation of human dendritic cells by type I IFN: synergistic interaction with TLR4 but not TLR3 agonists.
Authors: Walker J, Tough DF
Eur. J. Immunol., 2006-07-01;36(7):1827-36.
Species: Human
Sample Types: Cell Culture Supernates
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Prediction of acute renal allograft rejection by urinary monokine induced by IFN-gamma (MIG).
Authors: Hauser IA, Spiegler S, Kiss E, Gauer S, Sichler O, Scheuermann EH, Ackermann H, Pfeilschifter JM, Geiger H, Grone HJ, Radeke HH
J. Am. Soc. Nephrol., 2005-04-27;16(6):1849-58.
Species: Human
Sample Types: Urine
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Manipulation of host cytokine network by ticks: a potential gateway for pathogen transmission.
Authors: Hajnicka V, Vancova I, Kocakova P, Slovak M, Gasperik J, Slavikova M, Hails RS, Labuda M, Nuttall PA
Parasitology, 2005-03-01;130(0):333-42.
Species: Human
Sample Types: Saliva
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Secretion of monocyte chemoattractant protein-1 by endothelial cells of the bovine corpus luteum: regulation by cytokines but not prostaglandin F2alpha.
Authors: Cavicchio VA, Pru JK, Davis BS, Davis JS, Rueda BR, Townson DH
Endocrinology, 2002-09-01;143(9):3582-9.
Species: Bovine
Sample Types: Cell Culture Supernates
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Differential production of RANTES and MCP-1 in synovial fluid from the inflamed human knee.
Authors: Conti P, Reale M, Barbacane RC, Castellani ML, Orso C
Immunol. Lett., 2002-02-01;80(2):105-11.
Species: Human
Sample Types: Serum
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Human mesenchymal stem cells
We used human plasma neat and it work well, standard curve covers well the whole range
Detection of CCL2 released by human CAR-T cells producing CCL2 (PMC653, PMC654) or a different chemokine (PMC650).
Worked OK but the standard curve was slightly depressed compared to other duosets ive used
Suitable for urine use.
We used human serum without dilution. The result was very good and reproducible.