Human CCL3/MIP-1 alpha DuoSet ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human CCL3/MIP-1alpha. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.
Product Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Kit Content
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.
The components listed above may be purchased separately:
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4
Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990)
Plate Sealers: ELISA Plate Sealers (Catalog # DY992)
Scientific Data
Product Datasheets
Preparation and Storage
Background: CCL3/MIP-1 alpha
MIP-1 alpha (macrophage inflammatory protein 1 alpha) is a member of the CC or beta chemokine subfamily that was originally purified from the conditioned media of an LPS-stimulated murine macrophage cell line. MIP-1 alpha acts as a chemoattractant to a variety of cells including monocytes, T cells, B cells and eosinophils.
The two human MIP-1 alpha genes arise by duplication/mutation. They code for MIP-1 alpha isoforms CCL3/LD78a and CCL3L1/LD78b, which share 94% amino acid sequence homology. Whereas the human CCL3/LD78a is a single-copy gene, the human CCL3L1/LD78b gene copy number varies within the population. Human CCL3L1/LD78b binds and signals through chemokine receptors CCR1, CCR5. When compared to CCL3/LD78a, CCL3L1/LD78b has higher binding affinity to CCR5, which also functions as a coreceptor for HIV-1 entry. The copy number of CCL3L1 is one of several genetic determinants of HIV-1 susceptibility.
Assay Procedure
GENERAL ELISA PROTOCOL
Plate Preparation
- Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
- Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
- Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
- Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.
Assay Procedure
- Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
- Repeat the aspiration/wash as in step 2 of Plate Preparation.
- Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
- Repeat the aspiration/wash as in step 2 of Plate Preparation.
- Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
- Repeat the aspiration/wash as in step 2.
- Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
- Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
- Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.
Citations for Human CCL3/MIP-1 alpha DuoSet ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Blocking Thromboxane-Prostanoid Receptor Signaling Attenuates Lipopolysaccharide- and Stearic Acid-Induced Inflammatory Response in Human PBMCs
Authors: Rajamanickam, V;Desouza, CV;Castillo, RT;Saraswathi, V;
Cells
Species: Human
Sample Types: Cell Culture Supernates
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Effect of progestin-based contraceptives on HIV-associated vaginal immune biomarkers and microbiome in adolescent girls
Authors: Nasr, MA;Aldous, A;Daniels, J;Joy, C;Capozzi, E;Yang, M;Moriarty, P;Emmanuel-Baker, V;Malcolm, S;Green, SJ;Gomez-Lobo, V;Ghosh, M;
PloS one
Species: Human
Sample Types: Vaginal Swab
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Empowering Naringin's Anti-Inflammatory Effects through Nanoencapsulation
Authors: Marinho, A;Seabra, CL;Lima, SAC;Lobo-da-Cunha, A;Reis, S;Nunes, C;
International journal of molecular sciences
Species: Human
Sample Types: Cell Culture Supernates
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Using the Traditional Ex Vivo Whole Blood Model to Discriminate Bacteria by Their Inducible Host Responses
Authors: Chick, HM;Rees, ME;Lewis, ML;Williams, LK;Bodger, O;Harris, LG;Rushton, S;Wilkinson, TS;
Biomedicines
Species: Human
Sample Types: Whole Blood
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Highly secreted tryptophanyl tRNA synthetase 1 as a potential theranostic target for hypercytokinemic severe sepsis
Authors: Kim, YT;Huh, JW;Choi, YH;Yoon, HK;Nguyen, TT;Chun, E;Jeong, G;Park, S;Ahn, S;Lee, WK;Noh, YW;Lee, KS;Ahn, HS;Lee, C;Lee, SM;Kim, KS;Suh, GJ;Jeon, K;Kim, S;Jin, M;
EMBO molecular medicine
Species: Mouse
Sample Types: Plasma, Peritoneal Lavage Fluid
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Ventilatory support and inflammatory peptides in hospitalised patients with COVID-19: A prospective cohort trial
Authors: Gysan, MR;Milacek, C;Bal, C;Zech, A;Brugger, J;Milos, RI;Antoniewicz, L;Idzko, M;Gompelmann, D;
PloS one
Species: Human
Sample Types: Serum
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ADS024, a Bacillus velezensis strain, protects human colonic epithelial cells against C. difficile toxin-mediated apoptosis
Authors: Y Xie, A Chupina Es, B Nelson, H Feng, C Pothoulaki, L Chesnel, HW Koon
Frontiers in Microbiology, 2023-01-10;13(0):1072534.
Species: Human
Sample Types: Cell Culture Supernates
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Tryptophan-dependent and -independent secretions of tryptophanyl- tRNA synthetase mediate innate inflammatory responses
Authors: TTT Nguyen, YH Choi, WK Lee, Y Ji, E Chun, YH Kim, JE Lee, HS Jung, JH Suh, S Kim, M Jin
Cell Reports, 2022-12-30;42(1):111905.
Species: Human
Sample Types: Cell Culture Supernates
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Exercise training-induced changes in immunometabolic markers in youth badminton athletes
Authors: FE Rossi, AJ Maldonado, JM Cholewa, SLG Ribeiro, CA de Araújo, C Figueiredo, T Reichel, K Krüger, FS Lira, LG Minuzzi
Scientific Reports, 2022-09-15;12(1):15539.
Species: Human
Sample Types: Plasma
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Activation of the Innate Immune Checkpoint CLEC5A on Myeloid Cells in the Absence of Danger Signals Modulates Macrophages' Function but Does Not Trigger the Adaptive T Cell Immune Response
Authors: MJ Tosiek, K Groesser, A Pekcec, M Zwirek, G Murugesan, E Borges
Journal of Immunology Research, 2022-02-25;2022(0):9926305.
Species: Human
Sample Types: Cell Culture Supernates
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Inflammatory responses to metal oxide ceramic nanopowders
Authors: S Jamieson, A Mawdesley, D Deehan, J Kirby, J Holland, A Tyson-Capp
Scientific Reports, 2021-05-18;11(1):10531.
Species: Human
Sample Types: Cell Culture Supernates
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Systems view of Bordetella pertussis booster vaccination in adults primed with whole-cell vs. acellular vaccine
Authors: R da Silva A, F Soldevila, M Pomaznoy, M Babor, J Bennett, Y Tian, NN Khalil, Y Qian, A Mandava, RH Scheuerman, M Cortese, B Pulendran, CD Petro, AP Gilkes, LA Purcell, A Sette, B Peters
JCI Insight, 2021-04-08;0(0):.
Species: Human
Sample Types: Plasma
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Adipocytokines in Untreated Newly Diagnosed Rheumatoid Arthritis: Association with Circulating Chemokines and Markers of Inflammation
Authors: GK Vasileiadi, AC Lundell, Y Zhang, K Andersson, I Gjertsson, A Rudin, C Maglio
Biomolecules, 2021-02-21;11(2):.
Species: Human
Sample Types: Plasma
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Clinical Relevance of the Anti-inflammatory Effects of Roflumilast on Human Bronchus: Potentiation by a Long-Acting Beta-2-Agonist
Authors: H Salvator, A Buenestado, M Brollo, E Naline, T Victoni, E Longchamp, H Tenor, S Grassin-De, P Devillier
Frontiers in Pharmacology, 2020-12-08;11(0):598702.
Species: Human
Sample Types: Cell Culture Supernates
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High circulating SDF-1and MCP-1 levels and genetic variations in CXCL12, CCL2 and CCR5: Prognostic signature of immune recovery status in treated HIV-positive patients
Authors: E Yeregui, C Viladés, P Domingo, A Ceausu, YM Pacheco, S Veloso, A Inciarte, J Vidal-Gonz, M Peraire, C Perpiñán, V Falcó, J Masip, V Alba, M Vargas, A Martí, L Reverté, J Mallolas, F Vidal, J Peraire, A Rull
EBioMedicine, 2020-11-06;62(0):103077.
Species: Human
Sample Types: Plasma
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Resveratrol attenuates TLR-4 mediated inflammation and elicits therapeutic potential in models of sepsis
Authors: B Wang, GL Bellot, K Iskandar, TW Chong, FY Goh, JJ Tai, H Schwarz, SC Wong, S Pervaiz
Sci Rep, 2020-11-02;10(1):18837.
Species: Human
Sample Types: Cell Culture Supernates
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An observational study of innate immune responses in patients with acute appendicitis
Authors: T Peeters, S Martens, V D'Onofrio, MHT Stappers, JCH van der Hi, B Houben, R Achten, LAB Joosten, IC Gyssens
Sci Rep, 2020-10-15;10(1):17352.
Species: Human
Sample Types: Cell Culture Supernates
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Stearic Acid and TNF-&alpha Co-Operatively Potentiate MIP-1&alpha Production in Monocytic Cells via MyD88 Independent TLR4/TBK/IRF3 Signaling Pathway
Authors: S Kochumon, H Arefanian, R Azim, S Shenouda, T Jacob, N Abu Khalaf, F Al-Rashed, A Hasan, S Sindhu, F Al-Mulla, R Ahmad
Biomedicines, 2020-10-09;8(10):.
Species: Human
Sample Types: Cell Culture Supernates
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MIP-1alpha Expression Induced by Co-Stimulation of Human Monocytic Cells with Palmitate and TNF-alpha Involves the TLR4-IRF3 Pathway and Is Amplified by Oxidative Stress
Authors: S Sindhu, N Akhter, A Wilson, R Thomas, H Arefanian, A Al Madhoun, F Al-Mulla, R Ahmad
Cells, 2020-07-29;9(8):.
Species: Human
Sample Types: Cell Culture Supernates
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Biological Characterization of Commercial Recombinantly Expressed Immunomodulating Proteins Contaminated with Bacterial Products in the Year 2020: The SAA3 Case
Authors: S Abouelasra, M De Bondt, N Berghmans, M Gouwy, VLS de Oliveir, SC Oliveira, FA Amaral, P Proost, J Van Damme, S Struyf, M De Buck
Mediators Inflamm., 2020-07-06;2020(0):6087109.
Species: Human
Sample Types: Cell Culture Supernates
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Phenolic and Non-Polar Fractions of the Extracts from Fruits, Leaves, and Twigs of Elaeagnusrhamnoides (L.) A. Nelson-The Implications for Human Barrier Cells
Authors: B Sadowska, J Rywaniak, A Cichocka, K Cichocka, J ?uchowski, U Wójcik-Boj, M Wi?ckowska, B Ró?alska
Molecules, 2020-05-09;25(9):.
Species: Human
Sample Types: Cell Culture Supernates
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The human glomerular endothelial cells are potent pro-inflammatory contributors in an in vitro model of lupus nephritis
Authors: P Dimou, RD Wright, KL Budge, A Midgley, SC Satchell, M Peak, MW Beresford
Sci Rep, 2019-06-06;9(1):8348.
Species: Human
Sample Types: Cell Culture Supernates
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Bone marrow-derived mesenchymal stem cells promote colorectal cancer progression via CCR5
Authors: G Nishikawa, K Kawada, J Nakagawa, K Toda, R Ogawa, S Inamoto, R Mizuno, Y Itatani, Y Sakai
Cell Death Dis, 2019-03-19;10(4):264.
Species: Human
Sample Types: Serum
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Serum Apolipoprotein E and Other Inflammatory Markers Can Identify Non-Responding Patients to a Dendritic Cell Vaccine
Authors: H Leeman, E Kaminska, D Green, M Bodman-Smi, A Gravett, K Bodman-Smi, J Copier, G Coulton, A Fusi, AG Dalgleish
Transl Oncol, 2018-12-08;12(3):397-403.
Species: Human
Sample Types: Serum
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CUDC-907 blocks multiple pro-survival signals and abrogates microenvironment protection in CLL
Authors: Y Chen, C Peubez, V Smith, S Xiong, G Kocsis-Fod, B Kennedy, S Wagner, C Balotis, S Jayne, MJS Dyer, S Macip
J. Cell. Mol. Med., 2018-10-24;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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Broad adsorption of sepsis-related PAMP and DAMP molecules, mycotoxins, and cytokines from whole blood using CytoSorb� sorbent porous polymer beads
Authors: MC Gruda, KG Ruggeberg, P O'Sullivan, T Guliashvil, AR Scheirer, TD Golobish, VJ Capponi, PP Chan
PLoS ONE, 2018-01-25;13(1):e0191676.
Species: Human
Sample Types: Complex Sample Type
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Altered distribution of peripheral blood dendritic cell subsets in patients with pulmonary paracoccidioidomycosis
Authors: J Venturini, RS Cavalcante, DV Moris, M de Assis G, AD Levorato, KH Dos Reis, MSP de Arruda, RP Mendes
Acta Trop., 2017-06-09;0(0):.
Species: Human
Sample Types: Serum
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Relevance of CCL3/CCR5 axis in oral carcinogenesis
Authors: JM da Silva, TP Moreira Do, LM Sobral, CM Queiroz-Ju, MA Rachid, AEI Proudfoot, GP Garlet, AC Batista, MM Teixeira, AM Leopoldino, RC Russo, TA Silva
Oncotarget, 2017-04-06;8(31):51024-51036.
Species: Human
Sample Types: Cell Culture Supernates
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Biocompatibility of primers and an adhesive used for implant-retained maxillofacial prostheses: An in�vitro analysis
Authors: Daniela Micheline Dos Santos
J Prosthet Dent, 2016-11-09;0(0):.
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CD84 mediates CLL-microenvironment interactions
Oncogene, 2016-07-25;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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Neutrophil oxidative burst activates ATM to regulate cytokine production and apoptosis.
Authors: Harbort C, Soeiro-Pereira P, von Bernuth H, Kaindl A, Costa-Carvalho B, Condino-Neto A, Reichenbach J, Roesler J, Zychlinsky A, Amulic B
Blood, 2015-10-21;126(26):2842-51.
Species: Human
Sample Types: Cell Culture Supernates
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In vitro progesterone modulation on bacterial endotoxin-induced production of IL-1beta, TNFalpha, IL-6, IL-8, IL-10, MIP-1alpha, and MMP-9 in pre-labor human term placenta.
Authors: Garcia-Ruiz G, Flores-Espinosa P, Preciado-Martinez E, Bermejo-Martinez L, Espejel-Nunez A, Estrada-Gutierrez G, Maida-Claros R, Flores-Pliego A, Zaga-Clavellina V
Reprod Biol Endocrinol, 2015-10-07;13(0):115.
Species: Human
Sample Types: Cell Culture Supernates
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Human B cells induce dendritic cell maturation and favour Th2 polarization by inducing OX-40 ligand.
Authors: Maddur M, Sharma M, Hegde P, Stephen-Victor E, Pulendran B, Kaveri S, Bayry J
Nat Commun, 2014-06-09;5(0):4092.
Species: Human
Sample Types: Cell Culture Supernates
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Human osteoarthritic cartilage shows reduced in vivo expression of IL-4, a chondroprotective cytokine that differentially modulates IL-1beta-stimulated production of chemokines and matrix-degrading enzymes in vitro.
Authors: Assirelli E, Pulsatelli L, Dolzani P, Platano D, Olivotto E, Filardo G, Trisolino G, Facchini A, Borzi R, Meliconi R
PLoS ONE, 2014-05-12;9(5):e96925.
Species: Human
Sample Types: Cell Culture Supernates
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Selective activation of human dendritic cells by OM-85 through a NF-kB and MAPK dependent pathway.
Authors: Parola C, Salogni L, Vaira X, Scutera S, Somma P, Salvi V, Musso T, Tabbia G, Bardessono M, Pasquali C, Mantovani A, Sozzani S, Bosisio D
PLoS ONE, 2013-12-30;8(12):e82867.
Species: Human
Sample Types: Cell Culture Supernates
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Thrombin-cleaved fragments of osteopontin are overexpressed in malignant glial tumors and provide a molecular niche with survival advantage.
Authors: Yamaguchi Y, Shao Z, Sharif S, Du X, Myles T, Merchant M, Harsh G, Glantz M, Recht L, Morser J, Leung L
J Biol Chem, 2012-11-30;288(5):3097-111.
Species: Human
Sample Types: CSF
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Plasma levels of procalcitonin and eight additional inflammatory molecules in febrile neutropenic patients.
Authors: Neuenschwander LC, Bittencourt H, Ribeiro AF, Teixeira AL, Teixeira MM, Teixeira JC, Nobre V
Clinics (Sao Paulo), 2011-01-01;66(10):1699-705.
Species: Human
Sample Types: Plasma
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Abnormalities in serum chemokine levels in euthymic patients with bipolar disorder.
Authors: Brietzke E, Kauer-Sant'Anna M, Teixeira AL, Kapczinski F
Brain Behav. Immun., 2009-05-04;23(8):1079-82.
Species: Human
Sample Types: Serum
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Lenalidomide inhibits osteoclastogenesis, survival factors and bone-remodeling markers in multiple myeloma.
Authors: Breitkreutz I, Raab MS, Vallet S, Hideshima T, Raje N, Mitsiades C, Chauhan D, Okawa Y, Munshi NC, Richardson PG, Anderson KC
Leukemia, 2008-07-03;22(10):1925-32.
Species: Human
Sample Types: Cell Culture Supernates
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Altered levels of CC chemokines during pulmonary CMV predict BOS and mortality post-lung transplantation.
Authors: Weigt SS, Elashoff RM, Keane MP, Strieter RM, Gomperts BN, Xue YY, Ardehali A, Gregson AL, Kubak B, Fishbein MC, Saggar R, Ross DJ, Lynch JP, Zisman DA, Belperio JA
Am. J. Transplant., 2008-07-01;8(7):1512-22.
Species: Human
Sample Types: BALF
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Clinical, radiographic, and biochemical characterization of multiple myeloma patients with osteonecrosis of the jaw.
Authors: Raje N, Woo SB, Hande K, Yap JT, Richardson PG, Vallet S, Treister N, Hideshima T, Sheehy N, Chhetri S, Connell B, Xie W, Tai YT, Szot-Barnes A, Tian M, Schlossman RL, Weller E, Munshi NC, Van Den Abbeele AD, Anderson KC
Clin. Cancer Res., 2008-04-15;14(8):2387-95.
Species: Human
Sample Types: Serum
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p38 mitogen-activated protein kinase inhibitor LY2228820 enhances bortezomib-induced cytotoxicity and inhibits osteoclastogenesis in multiple myeloma; therapeutic implications.
Authors: Ishitsuka K, Hideshima T, Neri P, Vallet S, Shiraishi N, Okawa Y, Shen Z, Raje N, Kiziltepe T, Ocio EM, Chauhan D, Tassone P, Munshi N, Campbell RM, Dios AD, Shih C, Starling JJ, Tamura K, Anderson KC
Br. J. Haematol., 2008-04-07;141(5):598-606.
Species: Human
Sample Types: Cell Culture Supernates
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Association between the macrophage inflammatory protein-l alpha gene polymorphism and Alzheimer's disease in the Chinese population.
Authors: Li K, Dai D, Yao L, Gu X, Luan K, Tian W, Zhao Y, Wang B
Neurosci. Lett., 2008-01-10;433(2):125-8.
Species: Human
Sample Types: Plasma
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Impact of tobacco-smoke on key signaling pathways in the innate immune response in lung macrophages.
Authors: Birrell MA, Wong S, Catley MC, Belvisi MG
J. Cell. Physiol., 2008-01-01;214(1):27-37.
Species: Human
Sample Types: Cell Culture Supernates
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Increased serum levels of CCL11/eotaxin in schizophrenia.
Authors: Teixeira AL, Reis HJ, Nicolato R, Brito-Melo G, Correa H, Teixeira MM, Romano-Silva MA
Prog. Neuropsychopharmacol. Biol. Psychiatry, 2007-11-23;32(3):710-4.
Species: Human
Sample Types: Serum
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Targeting MEK1/2 blocks osteoclast differentiation, function and cytokine secretion in multiple myeloma.
Authors: Breitkreutz I, Raab MS, Vallet S, Hideshima T, Raje N, Chauhan D, Munshi NC, Richardson PG, Anderson KC
Br. J. Haematol., 2007-10-01;139(1):55-63.
Species: Human
Sample Types: Cell Culture Supernates
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Novel role for the liver X nuclear receptor in the suppression of lung inflammatory responses.
Authors: Birrell MA, Catley MC, Hardaker E, Wong S, Willson TM, McCluskie K, Leonard T, Farrow SN, Collins JL, Haj-Yahia S, Belvisi MG
J. Biol. Chem., 2007-08-31;282(44):31882-90.
Species: Human
Sample Types: Cell Culture Supernates
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Successful HAART is associated with high B-chemokine levels in chronic HIV type 1-infected patients.
Authors: Brito A, Almeida A, Gonsalez CR, Mendonca M, Ferreira F, Fernandes SS, Duarte AJ, Casseb J
AIDS Res. Hum. Retroviruses, 2007-07-01;23(7):906-12.
Species: Human
Sample Types: Cell Culture Supernates
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Modification of TLR-induced activation of human dendritic cells by type I IFN: synergistic interaction with TLR4 but not TLR3 agonists.
Authors: Walker J, Tough DF
Eur. J. Immunol., 2006-07-01;36(7):1827-36.
Species: Human
Sample Types: Cell Culture Supernates
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Role of matrix metalloproteinases in the inflammatory response in human airway cell-based assays and in rodent models of airway disease.
Authors: Birrell MA, Wong S, Dekkak A, De Alba J, Haj-Yahia S, Belvisi MG
J. Pharmacol. Exp. Ther., 2006-05-11;318(2):741-50.
Species: Human
Sample Types: Cell Culture Supernates
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Manipulation of host cytokine network by ticks: a potential gateway for pathogen transmission.
Authors: Hajnicka V, Vancova I, Kocakova P, Slovak M, Gasperik J, Slavikova M, Hails RS, Labuda M, Nuttall PA
Parasitology, 2005-03-01;130(0):333-42.
Species: Human
Sample Types: Saliva
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Involvement of Fc gamma RI (CD64) in the mechanism of HIV-1 inhibition by polyclonal IgG purified from infected patients in cultured monocyte-derived macrophages.
Authors: Holl V, Hemmerter S, Burrer R, Schmidt S, Bohbot A, Aubertin AM, Moog C
J. Immunol., 2004-11-15;173(10):6274-83.
Species: Human
Sample Types: Cell Culture Supernates
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Presence of dendritic cells, MCP-1, and activated microglia/macrophages in amyotrophic lateral sclerosis spinal cord tissue.
Authors: Henkel JS, Engelhardt JI, Siklos L, Simpson EP, Kim SH, Pan T, Goodman JC, Siddique T, Beers DR, Appel SH
Ann. Neurol., 2004-02-01;55(2):221-35.
Species: Human
Sample Types: CSF
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Virally stimulated plasmacytoid dendritic cells produce chemokines and induce migration of T and NK cells.
Authors: Megjugorac NJ, Young HA, Amrute SB, Olshalsky SL, Fitzgerald-Bocarsly P
J. Leukoc. Biol., 2004-01-23;75(3):504-14.
Species: Human
Sample Types: Cell Culture Supernates
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Gene copy number regulates the production of the human chemokine CCL3-L1.
Authors: Townson JR, Barcellos LF, Nibbs RJ
Eur. J. Immunol., 2002-10-01;32(10):3016-26.
Species: Human
Sample Types: Cell Culture Supernates
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