Human/Primate CCL5/RANTES Antibody

Catalog # Availability Size / Price Qty
MAB678-SP
MAB678-100
MAB678-500
Detection of Human CCL5/RANTES by Functional
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Product Details
Citations (38)
FAQs
Supplemental Products
Reviews (4)

Human/Primate CCL5/RANTES Antibody Summary

Species Reactivity
Human, Primate
Specificity
Detects human and primate CCL5/RANTES in direct ELISAs and Western blots. When used in combination with the biotinylated anti-human/primate CCL5/RANTES detection antibody (Catalog # BAF278) in sandwich ELISAs, no significant cross-reactivity or interference is observed with recombinant human (rh) ANG, rhCNTF, rh beta -ECGF, rhEGF, mouse (m) EGF, rhEpo, bovine (b) FGF acidic, rhFGF acidic, bFGF basic, rhFGF basic, rhFGF-4, rhG-CSF, rhGM-CSF, recombinant mouse (rm) GM-CSF, rhGRO alpha, rhIFN-gamma, rhIGF-I, rhIGF-II, rhIL-1 alpha, rmIL-1 alpha, rhIL-1 beta, rmIL‑1 beta, rhIL-1ra, recombinant rat (rr) IL-1ra, rhIL-2, rhIL-3, rmIL-3, rmIL-4, rhIL-5, rmIL-5, rhIL-6, rmIL-6, rhIL-7, rmIL-7, rhIL-8, rhIL-9, rmIL-9, rhIL‑10, rhIL-11, rhLIF, rhMCP‑1, rhM-CSF, rhMIP-1 alpha, rmMIP-1 alpha, rhMIP-1 beta, rmMIP-1 beta, rhOSM, human (h) PDGF, pPDGF, rhPDGF-AA, rhPDGF‑AB, rhPDGF-BB, rhSCF, rmSCF, rhSLPI, rhTGF-alpha, pTGF-beta 1, hTGF-beta 1, rhTGF-beta 1, pTGF-beta 2, rhTGF-beta 2, recombinant canine TGF-beta 3, rhTGF-beta 3, recombinant amphibian TGF-beta 5, rhTNF-alpha, rmTNF-alpha, or rmTNF-beta.
Source
Monoclonal Mouse IgG1 Clone # 21418
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human CCL5/RANTES
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human CCL5/RANTES (Catalog # 278-RN)

Human/Primate CCL5/RANTES Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human/Primate CCL5/RANTES Biotinylated Antibody (Catalog # BAF278)

Standard: Recombinant Human CCL5/RANTES Protein (Catalog # 278-RN)

Neutralization
Measured by its ability to neutralize CCL5/RANTES-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR5. The Neutralization Dose (ND50) is typically 0.04-0.12 µg/mL in the presence of 10 ng/mL Recombinant Human CCL5/RANTES.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Functional Detection of Human CCL5/RANTES by Functional View Larger

Detection of Human CCL5/RANTES by Functional The expression of chemokine CCL5 is up-regulated in co-cultures of BMMSCs cells and OTSCC.Co-culture of OTSCC cells with BMMSCs results in the high expression of chemokine CCL5 in HSC-3 and SAS cells, but not in dysplastic DOK cells (A). Function-blocking monoclonal antibody against CCL5 (Mab CCL5) slightly inhibits BMMSC enhanced invasion area, but has no effect on the invasion depth, whereas the antibody against CXCL1 does not increase the inhibitory effect in OTSCC invasion (B, C). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24204919), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Human CCL5/RANTES by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human CCL5/RANTES by Immunocytochemistry/Immunofluorescence RANTES filaments are present after exposure to shear stress.(A) HUVECs were electroporated with DNA encoding RANTES, cultivated on coverslips before stimulation with TNF alpha + IFN gamma for 30 h. RANTESwas labeled with an anti-RANTES antibody (clone 21418), and the cover slipswere mounted in a laminar flow chamber. The flow rate was adjusted to mimicvessel wall shear stress of 1 dyne/cm2. The imageswere acquired by confocal microscopy before (left image, 0 min) andafter 4 min with exposure to flow forces (right image, 4 min).(B) HUVECs were treated as described in (A) except that they were notlabeled with anti-RANTES antibody before exposure to flow. Human peripheralblood mononuclear cells were labeled with an anti-CD45 antibody, resuspendedin medium and applied by a pump to the flow chamber(1 dyne/cm2). After 10 min, the cells werelabeled with a rabbit anti-RANTES antibody. Images were acquired bysequential scanning confocal microscopy. Arrows and arrow heads indicateRANTES filaments and RANTES positive platelets, respectively. Originalmagnification in all panels, × 100. Scale bars, 10 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25791723), licensed under a CC-BY license. Not internally tested by R&D Systems.

Neutralization View Larger

Neutralization by Human/Primate CCL5/RANTES Antibody. Human/Primate CCL5/RANTES Antibody (Catalog # MAB678) neutralizes Recombinant Human CCL5/RANTES (278-RN) induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CCR5. The Neutralization Dose (ND50) for this effect is typically 80.0-800 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL5/RANTES

CCL5, also known as RANTES (Regulated upon Activation, Normal T cell Expressed and presumably Secreted) is a member of the beta (C-C) chemokine subfamily. It binds and activates the chemokine receptors CCR1, 3 and 5.

Entrez Gene IDs
6352 (Human); 20304 (Mouse); 403522 (Canine); 493689 (Feline)
Alternate Names
CCL5; chemokine (C-C motif) ligand 5; D17S136Enormally T-expressed, and presumably secreted; EoCP; Eosinophil chemotactic cytokine; RANTES; SISd; SIS-delta; small inducible cytokine A5 (RANTES); small inducible cytokine subfamily A (Cys-Cys), member 5; Small-inducible cytokine A5; T cell-specific protein P228; T-cell specific protein p288; TCP228T-cell-specific protein RANTES

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Citations for Human/Primate CCL5/RANTES Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

38 Citations: Showing 1 - 10
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  1. Epigenetic Repression of STING by MYC Promotes Immune Evasion and Resistance to Immune Checkpoint Inhibitors in Triple-Negative Breast Cancer
    Authors: Kyung-min Lee, Chang-Ching Lin, Alberto Servetto, Joonbeom Bae, Vishal Kandagatla, Dan Ye et al.
    Cancer Immunology Research
  2. Modulation of the CCR5 Receptor/Ligand Axis by Seminal Plasma and the Utility of In Vitro versus In Vivo Models
    Authors: Jennifer A. Juno, Kathleen M. Wragg, Anne B. Kristensen, Wen Shi Lee, Kevin J. Selva, Renee M. van der Sluis et al.
    Journal of Virology
  3. Activation of cGMP/PKG/p65 signaling associated with PDE5‐Is downregulates CCL5 secretion by CD8 + T cells in benign prostatic hyperplasia
    Authors: Song Jin, Peng Xiang, Jie Liu, Yang Yang, Shuai Hu, Jindong Sheng et al.
    The Prostate
  4. Low intraprostatic DHT promotes the infiltration of CD8+ T cells in BPH tissues via modulation of CCL5 secretion
    Authors: Yu Fan, Shuai Hu, Jie Liu, Fei Xiao, Xin Li, Wei Yu et al.
    Mediators of Inflammation
  5. Cholinergic stimulation blocks endothelial cell activation and leukocyte recruitment during inflammation
    Authors: Rubina W. Saeed, Santosh Varma, Tina Peng-Nemeroff, Barbara Sherry, David Balakhaneh, Jared Huston et al.
    The Journal of Experimental Medicine
  6. Oligomerized, filamentous surface presentation of RANTES/CCL5 on vascular endothelial cells.
    Authors: Oynebraten I, Barois N, Bergeland T, Kuchler A, Bakke O, Haraldsen G
    Sci Rep, 2015-03-20;5(0):9261.
  7. A scalable low-cost cGMP process for clinical grade production of the HIV inhibitor 5P12-RANTES in Pichia pastoris
    Authors: Fabrice Cerini, Hubert Gaertner, Knut Madden, Ilya Tolstorukov, Scott Brown, Bram Laukens et al.
    Protein Expression and Purification
  8. Effect of teriparatide on drug treatment of tuberculous spondylitis: an experimental study
    Authors: S Lee, YJ Seo, JY Choi, X Che, HJ Kim, SY Eum, MS Hong, SK Lee, DC Cho
    Scientific Reports, 2022-12-15;12(1):21667.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  9. Cancer-associated fibroblast-derived CCL5 contributes to cisplatin resistance in A549 NSCLC cells partially through upregulation of lncRNA HOTAIR expression
    Authors: X Sun, Z Chen
    Oncology Letters, 2021-08-02;22(4):696.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  10. Dissecting spatial heterogeneity and the immune-evasion mechanism of CTCs by single-cell RNA-seq in hepatocellular carcinoma
    Authors: YF Sun, L Wu, SP Liu, MM Jiang, B Hu, KQ Zhou, W Guo, Y Xu, Y Zhong, XR Zhou, ZF Zhang, G Liu, S Liu, YH Shi, Y Ji, M Du, NN Li, GB Li, ZK Zhao, XY Huang, LQ Xu, QC Yu, DH Peng, SJ Qiu, HC Sun, M Dean, XD Wang, WY Chung, AR Dennison, J Zhou, Y Hou, J Fan, XR Yang
    Nature Communications, 2021-07-02;12(1):4091.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  11. Immune-Stimulatory Effects of Curcumin on the Tumor Microenvironment in Head and Neck Squamous Cell Carcinoma
    Authors: C Kötting, L Hofmann, R Lotfi, D Engelhardt, S Laban, PJ Schuler, TK Hoffmann, C Brunner, MN Theodoraki
    Cancers, 2021-03-16;13(6):.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  12. T cell-inducing vaccine durably prevents mucosal SHIV infection even with lower neutralizing antibody titers
    Authors: PS Arunachala, TP Charles, V Joag, VS Bollimpell, MKD Scott, F Wimmers, SL Burton, CC Labranche, C Petitdeman, S Gangadhara, TM Styles, CF Quarnstrom, KA Walter, TJ Ketas, T Legere, PB Jagadeesh, SP Kasturi, A Tsai, BZ Yeung, S Gupta, M Tomai, J Vasilakos, GM Shaw, CY Kang, JP Moore, S Subramania, P Khatri, D Montefiori, PA Kozlowski, CA Derdeyn, E Hunter, D Masopust, RR Amara, B Pulendran
    Nat. Med., 2020-05-11;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  13. DSE regulates the malignant characters of hepatocellular carcinoma cells by modulating CCL5/CCR1 axis
    Authors: WC Liao, HR Yen, CK Liao, TJ Tseng, CT Lan, CH Liu
    Am J Cancer Res, 2019-02-01;9(2):347-362.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  14. Distinct susceptibility of HIV vaccine vector-induced CD4 T cells to HIV infection
    Authors: S Auclair, F Liu, Q Niu, W Hou, G Churchyard, C Morgan, N Frahm, S Nitayaphan, P Pitisuthit, S Rerks-Ngar, JT Kimata, L Soong, G Franchini, M Robb, J Kim, N Michael, H Hu
    PLoS Pathog., 2018-02-23;14(2):e1006888.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  15. C-C motif ligand 5 promotes migration of prostate cancer cells in the prostate cancer bone metastasis microenvironment
    Authors: S Urata, K Izumi, K Hiratsuka, A Maolake, A Natsagdorj, K Shigehara, H Iwamoto, S Kadomoto, T Makino, R Naito, Y Kadono, WJ Lin, G Wufuer, K Narimoto, A Mizokami
    Cancer Sci., 2018-02-14;109(3):724-731.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  16. SPON2 Promotes M1-like Macrophage Recruitment and Inhibits Hepatocellular Carcinoma Metastasis by Distinct Integrin-Rho GTPase-Hippo Pathways
    Authors: YL Zhang, Q Li, XM Yang, F Fang, J Li, YH Wang, Q Yang, L Zhu, HZ Nie, XL Zhang, MX Feng, SH Jiang, GA Tian, LP Hu, HY Lee, SJ Lee, Q Xia, ZG Zhang
    Cancer Res., 2018-02-13;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  17. SPARCL1 suppresses osteosarcoma metastasis and recruits macrophages by activation of canonical WNT/?-catenin signaling through stabilization of the WNT-receptor complex
    Authors: SJ Zhao, YQ Jiang, NW Xu, Q Li, Q Zhang, SY Wang, J Li, YH Wang, YL Zhang, SH Jiang, YJ Wang, YJ Huang, XX Zhang, GA Tian, CC Zhang, YY Lv, M Dai, F Liu, R Zhang, D Zhou, ZG Zhang
    Oncogene, 2017-10-30;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  18. CD8+ T cells promote proliferation of benign prostatic hyperplasia epithelial cells under low androgen level via modulation of CCL5/STAT5/CCND1 signaling pathway
    Authors: Y Yang, S Hu, J Liu, Y Cui, Y Fan, T Lv, L Liu, J Li, Q He, W Han, W Yu, Y Sun, J Jin
    Sci Rep, 2017-02-20;7(0):42893.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  19. Caspase-8 Acts in a Non-enzymatic Role as a Scaffold for Assembly of a Pro-inflammatory FADDosome Complex upon TRAIL Stimulation
    Authors: CM Henry, SJ Martin
    Mol. Cell, 2017-02-16;65(4):715-729.e5.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  20. Maternal and cord plasma cytokine and chemokine profile in pregnancies complicated by asthma.
    Authors: Osei-Kumah A, Smith R, Clifton VL
    Cytokine, 2008-07-17;43(2):187-93.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  21. Relation of circulating concentrations of chemokine receptor CCR5 ligands to C-peptide, proinsulin and HbA1c and disease progression in type 1 diabetes.
    Authors: Pfleger C, Kaas A, Hansen L, Alizadeh B, Hougaard P, Holl R, Kolb H, Roep BO, Mortensen HB, Schloot NC
    Clin. Immunol., 2008-04-22;128(1):57-65.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  22. Development and validation of sandwich ELISA microarrays with minimal assay interference.
    Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
    J. Proteome Res., 2008-04-19;7(6):2406-14.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Microarray Development
  23. Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
    Authors: Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB
    AIDS Res. Hum. Retroviruses, 2008-03-01;24(3):453-62.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  24. Expression of CCR5 receptors on Reed-Sternberg cells and Hodgkin lymphoma cell lines: involvement of CCL5/Rantes in tumor cell growth and microenvironmental interactions.
    Authors: Aldinucci D, Lorenzon D, Cattaruzza L, Pinto A, Gloghini A, Carbone A, Colombatti A
    Int. J. Cancer, 2008-02-15;122(4):769-76.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  25. Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines.
    Authors: Qui H, Ferrell EP, Nolan N, Phelps BH, Tabibiazar R, Whitney DH, Naelfski EA
    Clin. Chem., 2007-11-01;53(11):2010-2.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  26. Ultrasensitive flow-based immunoassays using single-molecule counting.
    Authors: Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, Goix P
    Clin. Chem., 2007-09-21;53(11):1990-5.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  27. Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
    Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
    Blood, 2007-02-08;109(10):4272-9.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  28. HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1.
    Authors: Grivel JC, Elliott J, Lisco A, Biancotto A, Condack C, Shattock RJ, McGowan I, Margolis L, Anton P
    AIDS, 2007;21(10):1263-72.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  29. Human Vgamma2Vdelta2 T cells contain cytoplasmic RANTES.
    Authors: Tikhonov I, Deetz CO, Paca R, Berg S, Lukyanenko V, Lim JK, Pauza CD
    Int. Immunol., 2006-06-01;18(8):1243-51.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Mass Spectometry
  30. Transforming growth factor beta 1(TGF-beta1) down-regulates TNFalpha-induced RANTES production in rheumatoid synovial fibroblasts through NF-kappaB-mediated transcriptional repression.
    Authors: Cho ML, Min SY, Chang SH, Kim KW, Heo SB, Lee SH, Park SH, Cho CS, Kim HY
    Immunol. Lett., 2006-03-03;105(2):159-66.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  31. Combination therapy: Synergistic suppression of virus-induced chemokines in airway epithelial cells.
    Authors: Edwards MR, Johnson MW, Johnston SL
    Am. J. Respir. Cell Mol. Biol., 2006-01-19;34(5):616-24.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  32. Changes in soluble factor-mediated CD8+ cell-derived antiviral activity in cynomolgus macaques infected with simian immunodeficiency virus SIVmac251: relationship to biological markers of progression.
    Authors: Dioszeghy V, Benlhassan-Chahour K, Delache B, Dereuddre-Bosquet N, Aubenque C, Gras G, Le Grand R, Vaslin B
    J. Virol., 2006-01-01;80(1):236-45.
    Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  33. Secretion of oncostatin M by infiltrating neutrophils: regulation of IL-6 and chemokine expression in human mesothelial cells.
    Authors: Hurst SM, McLoughlin RM, Monslow J, Owens S, Morgan L, Fuller GM, Topley N, Jones SA
    J. Immunol., 2002-11-01;169(9):5244-51.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  34. Selective induction of eotaxin release by interleukin-13 or interleukin-4 in human airway smooth muscle cells is synergistic with interleukin-1beta and is mediated by the interleukin-4 receptor alpha-chain.
    Authors: Hirst SJ, Hallsworth MP, Peng Q
    Am. J. Respir. Crit. Care Med., 2002-04-15;165(8):1161-71.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  35. Il-6 and its soluble receptor orchestrate a temporal switch in the pattern of leukocyte recruitment seen during acute inflammation.
    Authors: Hurst SM, Wilkinson TS, McLoughlin RM, Jones S, Horiuchi S, Yamamoto N, Rose-John S, Fuller GM, Topley N, Jones SA
    Immunity, 2001-06-01;14(6):705-14.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  36. Chemoattractant factors in breast milk from allergic and nonallergic mothers.
    Authors: Bottcher MF, Jenmalm MC, Bjorksten B, Garofalo RP
    Pediatr. Res., 2000-05-01;47(5):592-7.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  37. Pro-inflammatory mediators released by activated monocytes promote aortic valve fibrocalcific activity
    Authors: Peijian Zhang, Erlinda The, Zichao Luo, Yufeng Zhai, Qingzhou Yao, Lihua Ao et al.
    Molecular Medicine
  38. Human Bone Marrow Mesenchymal Stem Cells Induce Collagen Production and Tongue Cancer Invasion
    Authors: Sirpa Salo, Carolina Bitu, Kalle Merkku, Pia Nyberg, Ibrahim O Bello, Jussi Vuoristo et al.
    PLoS ONE

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Reviews for Human/Primate CCL5/RANTES Antibody

Average Rating: 4.5 (Based on 4 Reviews)

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Human/Primate CCL5/RANTES Antibody
By Katie Myers on 11/28/2022
Application: ELISA Sample Tested: Serum Species: Primate

Used as a capture in our NHP ELISA


Human/Primate CCL5/RANTES Antibody
By Anonymous on 07/24/2018
Application: ELISA Sample Tested: EDTA Plasma Species: Human

Human/Primate CCL5/RANTES Antibody
By Balaji Mahender on 12/06/2017
Application: ELISA Sample Tested: EDTA Plasma Species: Human

Human/Primate CCL5/RANTES Antibody
By Anonymous on 11/22/2017
Application: ELISA Sample Tested: Serum and Plasma Species: Human

The RANTES ELISA we made used MAG678 as the capture and BAF278 as the detection. Dilutional linearity of samples wasn't great but otherwise the assay worked very well.