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Human CCL5/RANTES Antibody

Catalog #: MAB278
14 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB278-SP
MAB278-500
MAB278-100
Chemotaxis Induced by CCL5/RANTES and Neutralization by Human CCL5/RANTES Antibody.
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Product Details
Citations (14)
FAQs
Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CCL5/RANTES Antibody Summary

Species Reactivity
Human
Specificity
Detects human CCL5/RANTES in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with other chemokines, including recombinant human CCL2, 3, 4,  recombinant mouse (rm) CCL3, and rmCCL4.
Source
Monoclonal Mouse IgG1 Clone # 21445
Purification
Protein A or G purified from ascites
Immunogen
E. coli-derived recombinant human CCL5/RANTES
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human CCL5/RANTES (Catalog # 278-RN)
Immunohistochemistry
8-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Human CCL5/RANTES transfected NS0 cells fixed with paraformaldehyde and permeabilized with saponin
Neutralization
Measured by its ability to neutralize CCL5/RANTES-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR5. The Neutralization Dose (ND50) is typically 0.1-0.4 µg/mL in the presence of 0.01 µg/mL Recombinant Human CCL5/RANTES.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by CCL5/RANTES and Neutralization by Human CCL5/RANTES Antibody. View Larger

Chemotaxis Induced by CCL5/RANTES and Neutralization by Human CCL5/RANTES Antibody. Recombinant Human CCL5/RANTES (Catalog # 278-RN) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL5/RANTES (0.01 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL5/RANTES Monoclonal Antibody (Catalog # MAB278). The ND50 is typically 0.1-0.4 µg/mL.

Immunohistochemistry CCL5/RANTES antibody in Mouse Small Intestine by Immunohistochemistry (IHC-Fr). View Larger

CCL5/RANTES in Mouse Small Intestine. CCL5/RANTES was detected in perfusion fixed frozen sections of mouse small intestine using Mouse Anti-Human CCL5/RANTES Monoclonal Antibody (Catalog # MAB278) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL5/RANTES

RANTES (Regulated upon Activation, Normal T cell Expressed and presumably Secreted) is a member of the beta (C-C) chemokine subfamily and is now designated CCL5. It binds and activates the chemokine receptors CCR1, 3 and 5.

Entrez Gene IDs
6352 (Human); 20304 (Mouse); 403522 (Canine); 493689 (Feline)
Alternate Names
CCL5; chemokine (C-C motif) ligand 5; D17S136Enormally T-expressed, and presumably secreted; EoCP; Eosinophil chemotactic cytokine; RANTES; SISd; SIS-delta; small inducible cytokine A5 (RANTES); small inducible cytokine subfamily A (Cys-Cys), member 5; Small-inducible cytokine A5; T cell-specific protein P228; T-cell specific protein p288; TCP228T-cell-specific protein RANTES

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Citations for Human CCL5/RANTES Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. Preterm Birth Is Associated With Immune Dysregulation Which Persists in Infants Exposed to Histologic Chorioamnionitis
    Authors: Gemma Sullivan, Paola Galdi, Nis Borbye-Lorenzen, David Q. Stoye, Gillian J. Lamb, Margaret J. Evans et al.
    Frontiers in Immunology
  2. A Novel Highly Potent Therapeutic Antibody Neutralizes Multiple Human Chemokines and Mimics Viral Immune Modulation
    Authors: Michelle L. Scalley-Kim, Bruce W. Hess, Ryan L. Kelly, Anne-Rachel F. Krostag, Kurt H. Lustig, John S. Marken et al.
    PLoS ONE
  3. Downregulation of alpha ‐ l ‐fucosidase 1 suppresses glioma progression by enhancing autophagy and inhibiting macrophage infiltration
    Authors: Lixia Xu, Zhenwei Li, Sirong Song, Qian Chen, Lidong Mo, Chen Wang et al.
    Cancer Science
  4. Chemokine positioning determines mutually exclusive roles for their receptors in extravasation of pathogenic human T cells
    Authors: F Parween, SP Singh, HH Zhang, N Kathuria, FA Otaizo-Car, A Shamsaddin, PJ Gardina, S Ganesan, J Kabat, HA Lorenzi, TG Myers, JM Farber
    bioRxiv : the preprint server for biology, 2023-02-13;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. DNA hypomethylation promotes transposable element expression and activation of immune signaling in renal cell cancer
    Authors: AA de Cubas, W Dunker, A Zaninovich, RA Hongo, A Bhatia, A Panda, KE Beckermann, G Bhanot, S Ganesan, J Karijolich, WK Rathmell
    JCI Insight, 2020-06-04;5(11):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Biological and Clinical Significance of the CCR5/CCL5 Axis in Hepatocellular Carcinoma
    Authors: SK Singh, MK Mishra, BM Rivers, JB Gordetsky, S Bae, R Singh
    Cancers (Basel), 2020-04-05;12(4):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  7. Pathological study of archival lung tissues from five fatal cases of avian H5N1 influenza in Vietnam.
    Authors: Nakajima N, Van Tin N, Sato Y, Thach H, Katano H, Diep P, Kumasaka T, Thuy N, Hasegawa H, San L, Kawachi S, Liem N, Suzuki K, Sata T
    Mod Pathol, 2012-11-23;26(3):357-69.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  8. Up-regulation of platelet activation in hemophilia A.
    Authors: van Bladel ER, Roest M
    Haematologica, 2011-03-21;0(0):.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  9. Pre-eclampsia is associated with dendritic cell recruitment into the uterine decidua.
    Authors: Huang SJ, Chen CP, Schatz F, Rahman M, Abrahams VM, Lockwood CJ
    J. Pathol., 2008-02-01;214(3):328-36.
    Species: Human
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: ELISA Development, IHC-Fr
  10. Endothelial damage from cytomegalovirus-specific host immune response can be prevented by targeted disruption of fractalkine-CX3CR1 interaction.
    Authors: Bolovan-Fritts CA, Spector SA
    Blood, 2007-09-25;111(1):175-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  11. Chemokine and chemokine receptor expression in paired peripheral blood mononuclear cells and synovial tissue of patients with rheumatoid arthritis, osteoarthritis, and reactive arthritis.
    Authors: Haringman JJ, Smeets TJ, Reinders-Blankert P
    Ann. Rheum. Dis., 2005-08-17;65(3):294-300.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  12. Rapid chemokine secretion from endothelial cells originates from 2 distinct compartments.
    Authors: Oynebraten I, Bakke O, Brandtzaeg P, Johansen FE, Haraldsen G
    Blood, 2004-03-25;104(2):314-20.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  13. Virally stimulated plasmacytoid dendritic cells produce chemokines and induce migration of T and NK cells.
    Authors: Megjugorac NJ, Young HA, Amrute SB, Olshalsky SL, Fitzgerald-Bocarsly P
    J. Leukoc. Biol., 2004-01-23;75(3):504-14.
    Species: Human
    Sample Types:
    Applications: Flow Cytometry
  14. Senescent Tumor Cells Build a Cytokine Shield in Colorectal Cancer
    Authors: Choi YW, Kim YH, Oh SY et al.
    Advanced Science

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Reviews for Human CCL5/RANTES Antibody

Average Rating: 4.5 (Based on 2 Reviews)

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Human CCL5/RANTES Antibody
By Anonymous on 09/18/2018
Application: ELISA Sample Tested: EDTA Plasma Species: Human
ELISA Human CCL5/RANTES Antibody MAB278
Human CCL5/RANTES Antibody
By Balaji Mahender on 12/06/2017
Application: ELISA Sample Tested: EDTA Plasma Species: Human