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Human CCR3 APC-conjugated Antibody

Catalog #: FAB155A
4 Citations Datasheet / COA / SDS
Clone 61828 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
FAB155A-025
FAB155A-100
Detection of CCR3 in Human Blood Granulocytes by Flow Cytometry.
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Product Details
Citations (4)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CCR3 APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CCR3 transfectants in flow cytometry. In flow cytometry, no cross-reactivity with CCR1, CCR2, CCR5, and CCR6 transfectants or the parental cell line is observed. Detects CCR3 on peripheral blood eosinophils.
Source
Monoclonal Rat IgG2A Clone # 61828
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Y3 rat myeloid cell line transfected with human CCR3
Accession # P51677
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CCR3 antibody in Human Blood Granulocytes antibody by Flow Cytometry. View Larger

Detection of CCR3 in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Mouse Anti-Human Integrin a4/CD49d PE-conjugated Monoclonal Antibody (Catalog # FAB1354P) and either (A) Rat Anti-Human CCR3 APC-conjugated Monoclonal Antibody (Catalog # FAB155A) or (B) Rat IgG2AAllophycocyanin Isotype Control (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR3

CCR3 is a G protein-linked seven transmembrane chemokine receptor that preferentially binds eotaxin and eotaxin-2.

Entrez Gene IDs
1232 (Human); 12771 (Mouse); 117027 (Rat); 448802 (Canine)
Alternate Names
b-chemokine receptor; CC chemokine receptor 3; C-C chemokine receptor type 3; C-C CKR-3; CCR3; CCR-3; CD193 antigen; CD193; chemokine (C-C motif) receptor 3; CKR3CC-CKR-3CMKBR3CD193; eosinophil CC chemokine receptor 3; Eosinophil eotaxin receptor; MGC102841

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Citations for Human CCR3 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Late IL-3-induced phenotypic and functional alterations in human basophils require continuous IL-3 receptor signaling
    J Leukoc Biol, 2016-07-21;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. A role for CCL28-CCR3 in T-cell homing to the human upper airway mucosa.
    Authors: Danilova, E, Skrindo, I, Gran, E, Hales, B J, Smith, W A, Jahnsen, J, Johansen, F E, Jahnsen, F L, Baekkevold, E S
    Mucosal Immunol, 2014-06-11;8(1):107-14.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. IL-27 in human secondary lymphoid organs attracts myeloid dendritic cells and impairs HLA class I-restricted antigen presentation.
    Authors: Morandi, Fabio, Di Carlo, Emma, Ferrone, Soldano, Petretto, Andrea, Pistoia, Vito, Airoldi, Irma
    J Immunol, 2014-02-19;192(6):2634-42.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Expression and functional heterogeneity of chemokine receptors CXCR4 and CXCR7 in primary patient-derived glioblastoma cells.
    Authors: Liu, Che, Pham, Kien, Luo, Defang, Reynolds, Brent A, Hothi, Parvinde, Foltz, Gregory, Harrison, Jeffrey
    PLoS ONE, 2013-03-21;8(3):e59750.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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