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Human CCR3 PE-conjugated Antibody

Catalog #: FAB155P
11 Citations Datasheet / COA / SDS
Clone 61828 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
FAB155P-025
FAB155P-100
Detection of CCR3 in Human Blood Granulocytes by Flow Cytometry.
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Product Details
Citations (11)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CCR3 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CCR3 transfectants in flow cytometry. In flow cytometry, no cross-reactivity with CCR1, CCR2, CCR5, and CCR6 transfectants or the parental cell line is observed. Detects CCR3 on peripheral blood eosinophils.
Source
Monoclonal Rat IgG2A Clone # 61828
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Y3 rat myeloid cell line transfected with human CCR3
Accession # P51677
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CCR3 antibody in Human Blood Granulocytes antibody by Flow Cytometry. View Larger

Detection of CCR3 in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Mouse Anti-Human Integrin a4/CD49d APC-conjugated Monoclonal Antibody (Catalog # FAB1354A) and either (A) Rat Anti-Human CCR3 PE-conjugated Monoclonal Antibody (Catalog # FAB155P) or (B) Rat IgG2APhycoerythrin Isotype Control (Catalog # IC006P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR3

CCR3 is a G protein-linked seven transmembrane chemokine receptor that preferentially binds eotaxin and eotaxin-2.

Entrez Gene IDs
1232 (Human); 12771 (Mouse); 117027 (Rat); 448802 (Canine)
Alternate Names
b-chemokine receptor; CC chemokine receptor 3; C-C chemokine receptor type 3; C-C CKR-3; CCR3; CCR-3; CD193 antigen; CD193; chemokine (C-C motif) receptor 3; CKR3CC-CKR-3CMKBR3CD193; eosinophil CC chemokine receptor 3; Eosinophil eotaxin receptor; MGC102841

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Citations for Human CCR3 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. A blocking monoclonal antibody to CCL24 alleviates liver fibrosis and inflammation in experimental models of liver damage
    Authors: Michal Segal-Salto, Neta Barashi, Avi Katav, Vicktoria Edelshtein, Arnon Aharon, Sharon Hashmueli et al.
    JHEP Reports
  2. Role of the atypical chemoattractant receptor CRAM in regulating CCL19 induced CCR7 responses in B-cell chronic lymphocytic leukemia
    Authors: Julie Catusse, Marion Leick, Mareike Groch, David J Clark, Maike V Buchner, Katja Zirlik et al.
    Molecular Cancer
  3. Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver.
    Authors: Oo YH, Weston CJ, Lalor PF, Curbishley SM, Withers DR, Reynolds GM, Shetty S, Harki J, Shaw JC, Eksteen B, Hubscher SG, Walker LS, Adams DH
    J. Immunol., 2010-02-17;184(6):2886-98.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. The Lupane-type Triterpene 30-Oxo-calenduladiol Is a CCR5 Antagonist with Anti-HIV-1 and Anti-chemotactic Activities.
    Authors: Barroso-Gonzalez J, El Jaber-Vazdekis N, Garcia-Exposito L, Machado JD, Zarate R, Ravelo AG, Estevez-Braun A, Valenzuela-Fernandez A
    J. Biol. Chem., 2009-04-22;284(24):16609-20.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Chemokine responsiveness of CD4+ CD25+ regulatory and CD4+ CD25- T cells from atopic and nonatopic donors.
    Authors: Ahern D, Lloyd CM, Robinson DS
    Allergy, 2009-02-07;64(8):1121-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases.
    Authors: Hartl D, Krauss-Etschmann S, Koller B, Hordijk PL, Kuijpers TW, Hoffmann F, Hector A, Eber E, Marcos V, Bittmann I, Eickelberg O, Griese M, Roos D
    J. Immunol., 2008-12-01;181(11):8053-67.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Chemokine production and chemokine receptor expression by human glioma cells: role of CXCL10 in tumour cell proliferation.
    Authors: Maru SV, Holloway KA, Flynn G, Lancashire CL, Loughlin AJ, Male DK, Romero IA
    J. Neuroimmunol., 2008-06-09;199(1):35-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Characterization of the human chemerin receptor--ChemR23/CMKLR1--as co-receptor for human and simian immunodeficiency virus infection, and identification of virus-binding receptor domains.
    Authors: Martensson UE, Fenyo EM, Olde B, Owman C
    Virology, 2006-08-10;355(1):6-17.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Chemokine receptors in head and neck cancer: association with metastatic spread and regulation during chemotherapy.
    Authors: Muller A, Sonkoly E, Eulert C, Gerber PA, Kubitza R, Schirlau K, Franken-Kunkel P, Poremba C, Snyderman C, Klotz LO, Ruzicka T, Bier H, Zlotnik A, Whiteside TL, Homey B, Hoffmann TK
    Int. J. Cancer, 2006-05-01;118(9):2147-57.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Chemokine receptor expression on human eosinophils from peripheral blood and bronchoalveolar lavage fluid after segmental antigen challenge.
    Authors: Liu LY, Jarjour NN, Busse WW, Kelly EA
    J. Allergy Clin. Immunol., 2003-09-01;112(3):556-62.
    Species: Human
    Sample Types: BALF
    Applications: Flow Cytometry
  11. Sequential involvement of CCR2 and CCR6 ligands for immature dendritic cell recruitment: possible role at inflamed epithelial surfaces.
    Authors: Vanbervliet B, Homey B, Durand I, Massacrier C, Ait-Yahia S, de Bouteiller O, Vicari A, Caux C
    Eur J Immunol, 2002-01-01;32(1):231-42.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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