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Human CCR7 APC-conjugated Antibody

Catalog #: FAB197A
24 Citations 1 Review Datasheet / COA / SDS
Clone 150503 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
FAB197A-025
FAB197A-100
Detection of CCR7 in Human Blood Lymphocytes by Flow Cytometry.
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Product Details
Citations (24)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CCR7 APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CCR7.
Source
Monoclonal Mouse IgG2A Clone # 150503
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human CCR7 transfectants
Met1-Pro378
Accession # AAA58615
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CCR7 antibody in Human Blood Lymphocytes antibody by Flow Cytometry. View Larger

Detection of CCR7 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB3791P) and either (A) Mouse Anti-Human CCR7 APC-conjugated Monoclonal Antibody (Catalog # FAB197A) or (B) Mouse IgG2AAllophycocyanin Isotype Control (Catalog # IC003A). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of CCR7 antibody in HEK293 Human Cell Line Transfected with Human CCR7 and eGFP antibody by Flow Cytometry. View Larger

Detection of CCR7 in HEK293 Human Cell Line Transfected with Human CCR7 and eGFP by Flow Cytometry. The specificity of Mouse Anti-Human CCR7 Clone 150503 was demonstrated by its ability to react with (A) HEK293 human embryonic kidney cell line transfected with human CCR7 and not react with (B) irrelevant HEK293 transfectants. Antibody binding was monitored using Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR7

CCR7 (Chemokine Receptor 7; also CD197) is a 7 transmembrane (7TM) G protein-coupled chemokine receptor for the homeostatic chemokines CCL19/MIP-3 beta and CCL21/6Ckine. CCL19 and CCL21 are constitutively expressed by high endothelial venule epithelial cells or fibroblastic reticular cells in secondary lymphoid organs. CCR7 is upregulated on dendritic cells, naïve and memory T cells, Treg, NK cells, and B cells following inflammatory stimulation. Its expression enables the function of immune cell trafficking to and retention in regional lymph nodes for expansion of the adaptive immune response. Human CCR7 shares 87% amino acid sequence identity with mouse CCR7.

Entrez Gene IDs
1236 (Human); 12775 (Mouse); 287673 (Rat)
Alternate Names
BLR2; BLR2C-C chemokine receptor type 7; C-C CKR-7; CC-CKR-7; CCR7; CCR-7; CD197 antigen; CD197; CDw197; CDw197CC chemokine receptor 7; chemokine (C-C motif) receptor 7; CMKBR7; CMKBR7chemokine (C-C) receptor 7; EBI1; EBI1EBV-induced G protein-coupled receptor 1; EBV-induced G-protein coupled receptor 1; Epstein-Barr virus induced gene 1; Epstein-Barr virus induced G-protein coupled receptor; Epstein-Barr virus-induced G-protein coupled receptor 1; EVI1; lymphocyte-specific G protein-coupled peptide receptor; MIP-3 beta receptor

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Citations for Human CCR7 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

24 Citations: Showing 1 - 10
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  1. Altered neutrophil responses to dengue virus serotype three: delayed apoptosis is regulated by stabilisation of Mcl-1
    Authors: Kamsom, C;Edwards, SW;Thaosing, J;Papalee, S;Pientong, C;Kurosu, T;Phanthanawiboon, S;
    Scientific reports
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Rab7b regulates dendritic cell migration by linking lysosomes to the actomyosin cytoskeleton
    Authors: K Vestre, I Persiconi, MB Distefano, N Mensali, NA Guadagno, M Bretou, S Wälchli, C Arnold-Sch, O Bakke, M Dalod, AM Lennon-Dum, C Progida
    Journal of Cell Science, 2021-09-24;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. A Versatile Toolkit for Semi-Automated Production of Fluorescent Chemokines to Study CCR7 Expression and Functions
    Authors: M Artinger, C Matti, OJ Gerken, CT Veldkamp, DF Legler
    International Journal of Molecular Sciences, 2021-04-16;22(8):.
    Species: Human
    Sample Types: Protein Lysate, Whole Cells
    Applications: Flow Cytometry, Western Blot
  4. Safety and feasibility of anti-CD19 CAR T cells with fully human binding domains in patients with B-cell lymphoma
    Authors: JN Brudno, N Lam, D Vanasse, YW Shen, JJ Rose, J Rossi, A Xue, A Bot, N Scholler, L Mikkilinen, M Roschewski, R Dean, R Cachau, P Youkhariba, R Patel, B Hansen, DF Stroncek, SA Rosenberg, RE Gress, JN Kochenderf
    Nat. Med., 2020-01-20;26(2):270-280.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Chemokine Receptor CCR7 Triggers an Endomembrane Signaling Complex for Spatial Rac Activation
    Authors: JM Laufer, MA Hauser, I Kindinger, V Purvanov, A Pauli, DF Legler
    Cell Rep, 2019-10-22;29(4):995-1009.e6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Biased Signaling of CCL21 and CCL19 Does Not Rely on N-Terminal Differences, but Markedly on the Chemokine Core Domains and Extracellular Loop 2 of CCR7
    Authors: Astrid S. Jørgensen, Olav Larsen, Edith Uetz-von Allmen, Michael Lückmann, Daniel F. Legler, Thomas M. Frimurer et al.
    Frontiers in Immunology
  7. Engineering of Nanobodies Recognizing the Human Chemokine Receptor CCR7
    Authors: BD Jakobs, L Spannagel, V Purvanov, E Uetz-von A, C Matti, DF Legler
    Int J Mol Sci, 2019-05-27;20(10):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. CCR7 Is Recruited to the Immunological Synapse, Acts as Co-stimulatory Molecule and Drives LFA-1 Clustering for Efficient T Cell Adhesion Through ZAP70
    Authors: Julia M. Laufer, Ilona Kindinger, Marc Artinger, Andreas Pauli, Daniel F. Legler
    Frontiers in Immunology
  9. Phosphatidylcholine and phosphatidylethanolamine plasmalogens in lipid loaded human macrophages
    Authors: S Wallner, E Orsó, M Grandl, T Konovalova, G Liebisch, G Schmitz
    PLoS ONE, 2018-10-11;13(10):e0205706.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Role of CXCL13 and CCL20 in the recruitment of B cells to inflammatory foci in chronic arthritis
    Authors: E Armas-Gonz, MJ Domínguez-, A Díaz-Martí, M Arce-Franc, J Castro-Her, G Danelon, V Hernández-, S Bustabad-R, A Cantabrana, M Uguccioni, F Díaz-Gonzá
    Arthritis Res. Ther., 2018-06-07;20(1):114.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. A unique signal sequence of the chemokine receptor CCR7 promotes package into COPII vesicles for efficient receptor trafficking
    Authors: E Uetz-von A, AV Rippl, H Farhan, DF Legler
    J. Leukoc. Biol., 2018-03-30;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Prospective evaluation of sequential treatment of sclerotic chronic graft versus host disease with rituximab and nilotinib
    Authors: L van der Wa, L Te Boome, M Schiffler, I Nijhof, M Schoordijk, S van Dorp, M van Dijk, R Raymakers, E Petersen, M de Witte, N de Jong, M Bellido, B Bär, E Meijer, J Kuball
    Bone Marrow Transplant., 2018-03-16;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Enhancement of cytokine-driven NK cell IFN-? production after vaccination of HCMV infected Africans
    Authors: A Darboe, E Danso, E Clarke, A Umesi, E Touray, R Wegmuller, SE Moore, EM Riley, MR Goodier
    Eur. J. Immunol., 2017-04-24;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. A high migratory capacity of donor T-cells in response to the lymph node homing receptor CCR7 increases the incidence and severity of GvHD
    Authors: I Portero-Sa, V G¢mez-Garc, C Cuesta-Mat, C Fern ndez-, L Vega-Piris, M Royg, B Colom-Fern, A Marcos-Jim, B Somovilla-, A Ram¡rez-Me, V L¢pez-Huet, A de Rosendo, A Kreutzman, C Mu¤oz-Call
    Bone Marrow Transplant, 2017-01-23;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. Codelivery of Envelope Protein in Alum with MVA Vaccine Induces CXCR3-Biased CXCR5+ and CXCR5- CD4 T Cell Responses in Rhesus Macaques.
    Authors: Iyer S, Gangadhara S, Victor B, Gomez R, Basu R, Hong J, Labranche C, Montefiori D, Villinger F, Moss B, Amara R
    J Immunol, 2015-06-26;195(3):994-1005.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Lymphocyte maintenance during healthy aging requires no substantial alterations in cellular turnover.
    Authors: Westera L, van Hoeven V, Drylewicz J, Spierenburg G, van Velzen J, de Boer R, Tesselaar K, Borghans J
    Aging Cell, 2015-01-28;14(2):219-27.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. Intramuscular delivery of heterodimeric IL-15 DNA in macaques produces systemic levels of bioactive cytokine inducing proliferation of NK and T cells.
    Authors: Bergamaschi C, Kulkarni V, Rosati M, Alicea C, Jalah R, Chen S, Bear J, Sardesai N, Valentin A, Felber B, Pavlakis G
    Gene Ther, 2014-10-02;22(1):76-86.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  18. Chemokines as novel and versatile reagents for flow cytometry and cell sorting.
    Authors: Le Brocq M, Fraser A, Cotton G, Woznica K, McCulloch C, Hewit K, McKimmie C, Nibbs R, Campbell J, Graham G
    J Immunol, 2014-05-21;192(12):6120-30.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  19. IFNgamma/IL-10 co-producing cells dominate the CD4 response to malaria in highly exposed children.
    Authors: Jagannathan P, Eccles-James I, Bowen K, Nankya F, Auma A, Wamala S, Ebusu C, Muhindo M, Arinaitwe E, Briggs J, Greenhouse B, Tappero J, Kamya M, Dorsey G, Feeney M
    PLoS Pathog, 2014-01-09;10(1):e1003864.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  20. Heterologous vaccination against human tuberculosis modulates antigen-specific CD4+ T-cell function.
    Authors: Dintwe O, Day C, Smit E, Nemes E, Gray C, Tameris M, McShane H, Mahomed H, Hanekom W, Scriba T
    Eur J Immunol, 2013-07-08;43(9):2409-20.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  21. Shaping of iNKT cell repertoire after unrelated cord blood transplantation.
    Authors: Beziat V, Nguyen S, Exley M, Achour A, Simon T, Chevallier P, Sirvent A, Vigouroux S, Debre P, Rio B, Vieillard V, Lubrano-Berthelier C, Alatrakchi N
    Clin. Immunol., 2010-02-13;135(3):364-73.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  22. Human dendritic cells acquire a semimature phenotype and lymph node homing potential through interaction with CD4+CD25+ regulatory T cells.
    Authors: Bayry J, Triebel F, Kaveri SV, Tough DF
    J. Immunol., 2007-04-01;178(7):4184-93.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  23. Trapping and apoptosis of novel subsets of memory T lymphocytes expressing CCR6 in the spleen of HIV-infected patients.
    Authors: Lecureuil C, Combadiere B, Mazoyer E, Bonduelle O, Samri A, Autran B, Debre P, Combadiere C
    Blood, 2006-12-29;109(9):3649-57.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  24. IL-7Ralpha expression on CD4+ T lymphocytes decreases with HIV disease progression and inversely correlates with immune activation.
    Authors: Koesters SA, Alimonti JB, Wachihi C, Matu L, Anzala O, Kimani J, Embree JE, Plummer FA, Fowke KR
    Eur. J. Immunol., 2006-02-01;36(2):336-44.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Human CCR7 APC-conjugated Antibody
By Anonymous on 12/11/2017
Application: Flow Sample Tested: Blood mononuclear cells (PBMCs) Species: Human