Home / CCR7 / Human CCR7 Fluorescein-conjugated Antibody

Human CCR7 Fluorescein-conjugated Antibody

Catalog #: FAB197F
77 Citations 1 Review Datasheet / COA / SDS
Clone 150503 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
FAB197F-025
FAB197F-100
Detection of CCR7 in Human Blood Lymphocytes by Flow Cytometry.
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Product Details
Citations (77)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CCR7 Fluorescein-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CCR7.
Source
Monoclonal Mouse IgG2A Clone # 150503
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human CCR7 transfectants
Met1-Pro378
Accession # AAA58615
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CCR7 antibody in Human Blood Lymphocytes antibody by Flow Cytometry. View Larger

Detection of CCR7 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB3791A) and either (A) Mouse Anti-Human CCR7 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB197F) or (B) Mouse IgG2AFluorescein Isotype Control (Catalog # IC003F). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of CCR7 antibody in HEK293 Human Cell Line Transfected with Human CCR7 and eGFP antibody by Flow Cytometry. View Larger

Detection of CCR7 in HEK293 Human Cell Line Transfected with Human CCR7 and eGFP by Flow Cytometry. The specificity of Mouse Anti-Human CCR7 Clone 150503 was demonstrated by its ability to react with (A) HEK293 human embryonic kidney cell line transfected with human CCR7 and not react with (B) irrelevant HEK293 transfectants. Antibody binding was monitored using Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Flow Cytometry Detection of Human CCR7 by Flow Cytometry View Larger

Detection of Human CCR7 by Flow Cytometry Frequency of peripheral T lymphocytes in hip fracture patients with and without depressive symptoms versus healthy controls. (a) The percentage of CD3+ve T cells in healthy controls: n = 37; hip fracture patients without depression (HF): n = 38 and hip fracture patients with depressive symptoms (HF + D): n = 29.The solid bar represents the mean value. (b) The mean absolute number of T cells in healthy controls: n = 20; hip fracture patients without depression (HF): n = 19 and hip fracture patients with depressive symptoms (HF + D): n = 15. Data are mean ± SEM. *p < .05 and **p < .01. Representative flow cytometry plots and gating strategy of PBMCs from a young individual stained with anti-CD3 and (c) anti-CD4, and CD8, (d) and anti-CD45RA and -CCR7 (e and f). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25628751), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR7

CCR7 (Chemokine Receptor 7; also CD197) is a 7 transmembrane (7TM) G protein-coupled chemokine receptor for the homeostatic chemokines CCL19/MIP-3 beta and CCL21/6Ckine. CCL19 and CCL21 are constitutively expressed by high endothelial venule epithelial cells or fibroblastic reticular cells in secondary lymphoid organs. CCR7 is upregulated on dendritic cells, naïve and memory T cells, Treg, NK cells, and B cells following inflammatory stimulation. Its expression enables the function of immune cell trafficking to and retention in regional lymph nodes for expansion of the adaptive immune response. Human CCR7 shares 87% amino acid sequence identity with mouse CCR7.

Entrez Gene IDs
1236 (Human); 12775 (Mouse); 287673 (Rat)
Alternate Names
BLR2; BLR2C-C chemokine receptor type 7; C-C CKR-7; CC-CKR-7; CCR7; CCR-7; CD197 antigen; CD197; CDw197; CDw197CC chemokine receptor 7; chemokine (C-C motif) receptor 7; CMKBR7; CMKBR7chemokine (C-C) receptor 7; EBI1; EBI1EBV-induced G protein-coupled receptor 1; EBV-induced G-protein coupled receptor 1; Epstein-Barr virus induced gene 1; Epstein-Barr virus induced G-protein coupled receptor; Epstein-Barr virus-induced G-protein coupled receptor 1; EVI1; lymphocyte-specific G protein-coupled peptide receptor; MIP-3 beta receptor

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Citations for Human CCR7 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

77 Citations: Showing 1 - 10
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  1. Scaffold‐Mediated Static Transduction of T Cells for CAR‐T Cell Therapy
    Authors: Pritha Agarwalla, Edikan A. Ogunnaike, Sarah Ahn, Frances S. Ligler, Gianpietro Dotti, Yevgeny Brudno
    Advanced Healthcare Materials
  2. p66Shc Deficiency in Chronic Lymphocytic Leukemia Promotes Chemokine Receptor Expression Through the ROS-Dependent Inhibition of NF-?B
    Authors: Tatangelo V, Boncompagni G, Capitani N et al.
    Frontiers in Oncology
  3. Type 1-programmed dendritic cells drive antigen-specific latency reversal and immune elimination of persistent HIV-1
    Authors: J Kristoff, ML Palma, TM Garcia-Bat, C Shen, N Sluis-Crem, P Gupta, CR Rinaldo, RB Mailliard
    EBioMedicine, 2019-04-02;0(0):.
  4. Leukocyte Imbalances in Mucopolysaccharidoses Patients
    Authors: Lopes, N;Maia, ML;Pereira, CS;Mondragão-Rodrigues, I;Martins, E;Ribeiro, R;Gaspar, A;Aguiar, P;Garcia, P;Cardoso, MT;Rodrigues, E;Leão-Teles, E;Giugliani, R;Coutinho, MF;Alves, S;Macedo, MF;
    Biomedicines
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. p66Shc Deficiency in Chronic Lymphocytic Leukemia Promotes Chemokine Receptor Expression Through the ROS-Dependent Inhibition of NF-?B
    Authors: Tatangelo V, Boncompagni G, Capitani N et al.
    Frontiers in Oncology
  6. Reconstitution of EBV-directed T cell immunity by adoptive transfer of peptide-stimulated T cells in a patient after allogeneic stem cell transplantation for AITL
    Authors: MF Lammoglia, J Ritter, R Gary, V Seitz, J Mautner, M Aigner, S Völkl, S Schaffer, S Moi, A Seegebarth, H Bruns, W Rösler, K Amann, M Büttner-He, S Hennig, A Mackensen, M Hummel, A Moosmann, A Gerbitz
    PloS Pathogens, 2022-04-22;18(4):e1010206.
    Species: Human
    Sample Types:
    Applications: Flow Cytometry
  7. Bioinstructive implantable scaffolds for rapid in vivo manufacture and release of CAR-T cells
    Authors: P Agarwalla, EA Ogunnaike, S Ahn, KA Froehlich, A Jansson, FS Ligler, G Dotti, Y Brudno
    Nature Biotechnology, 2022-03-24;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. TGF-beta 1 potentiates V gamma 9Vδ2 T cell adoptive immunotherapy of cancer
    Authors: Richard E. Beatson, Ana C. Parente-Pereira, Leena Halim, Domenico Cozzetto, Caroline Hull, Lynsey M. Whilding et al.
    Cell Reports Medicine
  9. TGF-beta 1 potentiates V gamma 9Vδ2 T cell adoptive immunotherapy of cancer
    Authors: Richard E. Beatson, Ana C. Parente-Pereira, Leena Halim, Domenico Cozzetto, Caroline Hull, Lynsey M. Whilding et al.
    Cell Reports Medicine
  10. BRAF and MEK Inhibitors Affect Dendritic-Cell Maturation and T-Cell Stimulation
    Authors: S Hoyer, V Eberlein, G Schuler, C Berking, L Heinzerlin, N Schaft, J Dörrie
    Oncogene, 2021-11-04;22(21):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. PD-1 is imprinted on cytomegalovirus-specific CD4+ T cells and attenuates Th1 cytokine production whilst maintaining cytotoxicity
    Authors: HM Parry, AC Dowell, J Zuo, K Verma, FAM Kinsella, J Begum, W Croft, A Sharma-Oat, G Pratt, P Moss
    PloS Pathogens, 2021-03-04;17(3):e1009349.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. SARS-CoV-2 induces human plasmacytoid pre-dendritic cell diversification via UNC93B and IRAK4
    Authors: F Onodi, L Bonnet-Mad, L Meertens, L Karpf, J Poirot, SY Zhang, C Picard, A Puel, E Jouanguy, Q Zhang, J Le Goff, JM Molina, C Delaugerre, JL Casanova, A Amara, V Soumelis
    bioRxiv : the preprint server for biology, 2021-01-08;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. IL-18 Responsiveness Defines Limitations in Immune Help for Specialized FcR&gamma- NK Cells
    Authors: RR Anderko, CR Rinaldo, RB Mailliard
    J Immunol, 2020-11-13;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Insight into the mechanism of cytotoxicity of membrane-permeant psoralenic Kv1.3 channel inhibitors by chemical dissection of a novel member of the family
    Authors: R Peruzzo, A Mattarei, M Azzolini, KA Becker-Fle, M Romio, G Rigoni, A Carrer, L Biasutto, S Parrasia, S Kadow, A Managò, A Urbani, A Rossa, G Semenzato, ME Soriano, L Trentin, S Ahmad, M Edwards, E Gulbins, C Paradisi, M Zoratti, L Leanza, I Szabò
    Redox Biol, 2020-09-06;37(0):101705.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  15. Chemokine Receptor CCR7 Triggers an Endomembrane Signaling Complex for Spatial Rac Activation
    Authors: JM Laufer, MA Hauser, I Kindinger, V Purvanov, A Pauli, DF Legler
    Cell Rep, 2019-10-22;29(4):995-1009.e6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. TCR activation mimics CD127lowPD-1high phenotype and functional alterations of T lymphocytes from septic shock patients
    Authors: J Mouillaux, C Allam, M Gossez, T Uberti, B Delwarde, J Hayman, T Rimmelé, J Textoris, G Monneret, E Peronnet, F Venet
    Crit Care, 2019-04-17;23(1):131.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. Cancer immunotherapy with T cells carrying bispecific receptors that mimic antibodies
    Authors: S Ahn, J Li, C Sun, K Gao, K Hirabayash, H Li, B Savoldo, R Liu, G Dotti
    Cancer Immunol Res, 2019-03-06;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: CAR-T (Flow Cytometry)
  18. CD226 opposes TIGIT to disrupt Tregs in melanoma
    Authors: J Fourcade, Z Sun, JM Chauvin, M Ka, D Davar, O Pagliano, H Wang, S Saada, C Menna, R Amin, C Sander, JM Kirkwood, AJ Korman, HM Zarour
    JCI Insight, 2018-07-26;3(14):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  19. Innate and adaptive immune dysregulation in critically ill ICU patients
    Authors: NA Duggal, C Snelson, U Shaheen, V Pearce, JM Lord
    Sci Rep, 2018-07-05;8(1):10186.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  20. Age-related differences in humoral and cellular immune responses after primary immunisation: indications for stratified vaccination schedules
    Authors: A Wagner, E Garner-Spi, J Jasinska, H Kollaritsc, K Stiasny, M Kundi, U Wiedermann
    Sci Rep, 2018-06-29;8(1):9825.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  21. Clinical-grade generation of peptide-stimulated CMV/EBV-specific T cells from G-CSF mobilized stem cell grafts
    Authors: R Gary, M Aigner, S Moi, S Schaffer, A Gottmann, S Maas, R Zimmermann, J Zingsem, J Strobel, A Mackensen, J Mautner, A Moosmann, A Gerbitz
    J Transl Med, 2018-05-09;16(1):124.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  22. PD-1 and PD-L1 Up-regulation Promotes T-cell Apoptosis in Gastric Adenocarcinoma
    Authors: YM Chiu, CL Tsai, JT Kao, CT Hsieh, DC Shieh, YJ Lee, GJ Tsay, KS Cheng, YY Wu
    Anticancer Res., 2018-04-01;38(4):2069-2078.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  23. Novel mechanisms to inhibit HIV reservoir seeding using Jak inhibitors
    Authors: C Gavegnano, JH Brehm, FP Dupuy, A Talla, SP Ribeiro, DA Kulpa, C Cameron, S Santos, SJ Hurwitz, VC Marconi, JP Routy, L Sabbagh, RF Schinazi, RP Sékaly
    PLoS Pathog., 2017-12-21;13(12):e1006740.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  24. PD-1 status in CD8(+) T cells associates with survival and anti-PD-1 therapeutic outcomes in head and neck cancer
    Authors: BA Kansy, F Concha-Ben, RM Srivastava, HB Jie, G Shayan, Y Lei, J Moskovitz, J Moy, J Li, S Brandau, S Lang, NC Schmitt, GJ Freeman, WE Gooding, DA Clump, RL Ferris
    Cancer Res., 2017-09-13;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  25. Prehospital immune responses and development of multiple organ dysfunction syndrome following traumatic injury: A prospective cohort study
    Authors: J Hazeldine, DN Naumann, E Toman, D Davies, JRB Bishop, Z Su, P Hampson, RJ Dinsdale, N Crombie, NA Duggal, P Harrison, A Belli, JM Lord
    PLoS Med., 2017-07-18;14(7):e1002338.
    Species: Human
    Sample Types: Whole Blood
    Applications: Flow Cytometry
  26. Regeneration of long-distance peripheral nerve defects after delayed reconstruction in healthy and diabetic rats is supported by immunomodulatory chitosan nerve guides
    Authors: L Stenberg, M Stö beta el, G Ronchi, S Geuna, Y Yin, S Mommert, L Mårtensson, J Metzen, C Grothe, LB Dahlin, K Haastert-T
    BMC Neurosci, 2017-07-18;18(1):53.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  27. pERK-dependent defective TCR-mediated activation of CD4(+) T cells in end-stage renal disease patients
    Authors: L Huang, NHR Litjens, NM Kannegiete, M Klepper, CC Baan, MGH Betjes
    Immun Ageing, 2017-06-19;14(0):14.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  28. Natural killer cell counts are associated with molecular relapse-free survival after imatinib discontinuation in chronic myeloid leukemia: the IMMUNOSTIM study
    Authors: D Rea, G Henry, Z Khaznadar, G Etienne, F Guilhot, F Nicolini, J Guilhot, P Rousselot, F Huguet, L Legros, M Gardembas, V Dubruille, A Guerci-Bre, A Charbonnie, F Maloisel, JC Ianotto, B Villemagne, FX Mahon, H Moins-Teis, N Dulphy, A Toubert
    Haematologica, 2017-05-18;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  29. NKG2D ligand targeted Bispecific T Cell Engagers lead to robust antitumor activity against diverse human tumors
    Authors: C Godbersen, TA Coupet, AM Huehls, T Zhang, MB Battles, JL Fisher, MS Ernstoff, CL Sentman
    Mol. Cancer Ther., 2017-05-12;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  30. Glatiramer Acetate, Dimethyl Fumarate, and Monomethyl Fumarate Upregulate the Expression of CCR10 on the Surface of Natural Killer Cells and Enhance Their Chemotaxis and Cytotoxicity
    Authors: Zaidoon Al-Jaderi
    Front Immunol, 2016-10-19;7(0):437.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  31. Tumor-infiltrating Tim-3(+) T cells proliferate avidly except when PD-1 is co-expressed: Evidence for intracellular cross talk
    Authors: Robert L Ferris
    Oncoimmunology, 2016-09-22;5(10):e1200778.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  32. Glycolysis determines dichotomous regulation of T cell subsets in hypoxia
    Authors: Yang Xu
    J Clin Invest, 2016-06-13;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  33. Effective Cytotoxic T Lymphocyte Targeting of Persistent HIV-1 during Antiretroviral Therapy Requires Priming of Naive CD8+ T Cells
    Authors: Kellie N Smith
    MBio, 2016-05-31;7(3):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  34. Envelope residue 375 substitutions in simian-human immunodeficiency viruses enhance CD4 binding and replication in rhesus macaques
    Authors: Hui Li
    Proc Natl Acad Sci USA, 2016-05-31;113(24):E3413-22.
    Species: Human, Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  35. Human liver-resident CD56(bright)/CD16(neg) NK cells are retained within hepatic sinusoids via the engagement of CCR5 and CXCR6 pathways.
    Authors: Hudspeth K, Donadon M, Cimino M, Pontarini E, Tentorio P, Preti M, Hong M, Bertoletti A, Bicciato S, Invernizzi P, Lugli E, Torzilli G, Gershwin M, Mavilio D
    J Autoimmun, 2015-08-30;66(0):40-50.
    Species: Human
    Sample Types:
    Applications: Flow Cytometry
  36. Human Non-neutralizing HIV-1 Envelope Monoclonal Antibodies Limit the Number of Founder Viruses during SHIV Mucosal Infection in Rhesus Macaques.
    Authors: Santra S, Tomaras G, Warrier R, Nicely N, Liao H, Pollara J, Liu P, Alam S, Zhang R, Cocklin S, Shen X, Duffy R, Xia S, Schutte R, Pemble Iv C, Dennison S, Li H, Chao A, Vidnovic K, Evans A, Klein K, Kumar A, Robinson J, Landucci G, Forthal D, Montefiori D, Kaewkungwal J, Nitayaphan S, Pitisuttithum P, Rerks-Ngarm S, Robb M, Michael N, Kim J, Soderberg K, Giorgi E, Blair L, Korber B, Moog C, Shattock R, Letvin N, Schmitz J, Moody M, Gao F, Ferrari G, Shaw G, Haynes B
    PLoS Pathog, 2015-08-03;11(8):e1005042.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  37. Evaluating Human T-Cell Therapy of Cytomegalovirus Organ Disease in HLA-Transgenic Mice.
    Authors: Thomas S, Klobuch S, Podlech J, Plachter B, Hoffmann P, Renzaho A, Theobald M, Reddehase M, Herr W, Lemmermann N
    PLoS Pathog, 2015-07-16;11(7):e1005049.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  38. Cytomegalovirus-Induced Expression of CD244 after Liver Transplantation Is Associated with CD8+ T Cell Hyporesponsiveness to Alloantigen.
    Authors: de Mare-Bredemeijer E, Shi X, Mancham S, van Gent R, van der Heide-Mulder M, de Boer R, Heemskerk M, de Jonge J, van der Laan L, Metselaar H, Kwekkeboom J
    J Immunol, 2015-07-13;195(4):1838-48.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  39. STAT3 is a critical cell-intrinsic regulator of human unconventional T cell numbers and function.
    Authors: Wilson R, Ives M, Rao G, Lau A, Payne K, Kobayashi M, Arkwright P, Peake J, Wong M, Adelstein S, Smart J, French M, Fulcher D, Picard C, Bustamante J, Boisson-Dupuis S, Gray P, Stepensky P, Warnatz K, Freeman A, Rossjohn J, McCluskey J, Holland S, Casanova J, Uzel G, Ma C, Tangye S, Deenick E
    J Exp Med, 2015-05-04;212(6):855-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  40. Lymphocyte gene expression signatures from patients and mouse models of hereditary hemochromatosis reveal a function of HFE as a negative regulator of CD8+ T-lymphocyte activation and differentiation in vivo.
    Authors: Costa M, Cruz E, Oliveira S, Benes V, Ivacevic T, Silva M, Vieira I, Dias F, Fonseca S, Goncalves M, Lima M, Leitao C, Muckenthaler M, Pinto J, Porto G
    PLoS ONE, 2015-04-16;10(4):e0124246.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  41. CD40L induces functional tunneling nanotube networks exclusively in dendritic cells programmed by mediators of type 1 immunity.
    Authors: Zaccard C, Watkins S, Kalinski P, Fecek R, Yates A, Salter R, Ayyavoo V, Rinaldo C, Mailliard R
    J Immunol, 2014-12-29;194(3):1047-56.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  42. Concurrent interaction of DCs with CD4(+) and CD8(+) T cells improves secondary CTL expansion: It takes three to tango.
    Authors: Hoyer S, Prommersberger S, Pfeiffer I, Schuler-Thurner B, Schuler G, Dorrie J, Schaft N
    Eur J Immunol, 2014-10-27;44(12):3543-59.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  43. Tetramer enrichment reveals the presence of phenotypically diverse hepatitis C virus-specific CD8+ T cells in chronic infection.
    Authors: Nitschke K, Flecken T, Schmidt J, Gostick E, Marget M, Neumann-Haefelin C, Blum H, Price D, Thimme R
    J Virol, 2014-10-15;89(1):25-34.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  44. Exhaustion of bacteria-specific CD4 T cells and microbial translocation in common variable immunodeficiency disorders.
    Authors: Perreau M, Vigano S, Bellanger F, Pellaton C, Buss G, Comte D, Roger T, Lacabaratz C, Bart P, Levy Y, Pantaleo G
    J Exp Med, 2014-09-15;211(10):2033-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  45. Tracking in vivo dynamics of NK cells transferred in patients undergoing stem cell transplantation.
    Authors: Killig M, Friedrichs B, Meisig J, Gentilini C, Bluthgen N, Loddenkemper C, Labopin M, Basara N, Pfrepper C, Niederwieser D, Uharek L, Romagnani C
    Eur J Immunol, 2014-06-30;44(9):2822-34.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  46. Cytomegalovirus drives Vdelta2neg gammadelta T cell inflation in many healthy virus carriers with increasing age.
    Authors: Alejenef A, Pachnio A, Halawi M, Christmas S, Moss P, Khan N
    Clin Exp Immunol, 2014-06-01;176(3):418-28.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  47. TCR affinity associated with functional differences between dominant and subdominant SIV epitope-specific CD8+ T cells in Mamu-A*01+ rhesus monkeys.
    Authors: Osuna C, Gonzalez A, Chang H, Hung A, Ehlinger E, Anasti K, Alam S, Letvin N
    PLoS Pathog, 2014-04-17;10(4):e1004069.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  48. MART-1 peptide vaccination plus IMP321 (LAG-3Ig fusion protein) in patients receiving autologous PBMCs after lymphodepletion: results of a Phase I trial.
    Authors: Romano E, Michielin O, Voelter V, Laurent J, Bichat H, Stravodimou A, Romero P, Speiser D, Triebel F, Leyvraz S, Harari A
    J Transl Med, 2014-04-12;12(0):97.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  49. Severe acute respiratory syndrome-coronavirus infection in aged nonhuman primates is associated with modulated pulmonary and systemic immune responses.
    Authors: Clay C, Donart N, Fomukong N, Knight J, Overheim K, Tipper J, Van Westrienen J, Hahn F, Harrod K
    Immun Ageing, 2014-03-19;11(1):4.
    Species: Primate - Chlorocebus pygerythrus (Vervet Monkey)
    Sample Types: Tissue Homogenates
    Applications: Flow Cytometry
  50. IL-27 in human secondary lymphoid organs attracts myeloid dendritic cells and impairs HLA class I-restricted antigen presentation.
    Authors: Morandi, Fabio, Di Carlo, Emma, Ferrone, Soldano, Petretto, Andrea, Pistoia, Vito, Airoldi, Irma
    J Immunol, 2014-02-19;192(6):2634-42.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  51. IL-12 directs further maturation of ex vivo differentiated NK cells with improved therapeutic potential.
    Authors: Lehmann, Dorit, Spanholtz, Jan, Sturtzel, Caterina, Tordoir, Marleen, Schlechta, Bernhard, Groenewegen, Dirk, Hofer, Erhard
    PLoS ONE, 2014-01-31;9(1):e87131.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  52. Peripheral blood CD56(bright) NK cells respond to stem cell factor and adhere to its membrane-bound form after upregulation of c-kit.
    Authors: Pradier A, Tabone-Eglinger S, Huber V, Bosshard C, Rigal E, Wehrle-Haller B, Roosnek E
    Eur J Immunol, 2013-11-20;44(2):511-20.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  53. Both HLA-B*57 and plasma HIV RNA levels contribute to the HIV-specific CD8+ T cell response in HIV controllers.
    Authors: Lecuroux C, Saez-Cirion A, Girault I, Versmisse P, Boufassa F, Avettand-Fenoel V, Rouzioux C, Meyer L, Pancino G, Lambotte O, Sinet M, Venet A
    J Virol, 2013-10-16;88(1):176-87.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  54. IL-18-based combinatorial adjuvants promote the intranodal production of CCL19 by NK cells and dendritic cells of cancer patients.
    Authors: Wong J, Muthuswamy R, Bartlett D, Kalinski P
    Oncoimmunology, 2013-09-12;2(9):e26245.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  55. Bone marrow T cells from the femur are similar to iliac crest derived cells in old age and represent a useful tool for studying the aged immune system.
    Authors: Pritz T, Landgraf-Rauf K, Herndler-Brandstetter D, Rauf R, Lair J, Gassner R, Weinberger B, Krismer M, Grubeck-Loebenstein B
    Immun Ageing, 2013-05-04;10(1):17.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  56. CCR5 is a receptor for Staphylococcus aureus leukotoxin ED.
    Authors: Alonzo, Francis, Kozhaya, Lina, Rawlings, Stephen, Reyes-Robles, Tamara, DuMont, Ashley L, Myszka, David G, Landau, Nathanie, Unutmaz, Derya, Torres, Victor J
    Nature, 2012-12-12;493(7430):51-5.
    Species: Human
    Sample Types: Whole Cells
  57. The CD46-Jagged1 interaction is critical for human TH1 immunity.
    Authors: Le Friec G, Sheppard D, Whiteman P, Karsten C, Shamoun S, Laing A, Bugeon L, Dallman M, Melchionna T, Chillakuri C, Smith R, Drouet C, Couzi L, Fremeaux-Bacchi V, Kohl J, Waddington S, McDonnell J, Baker A, Handford P, Lea S, Kemper C
    Nat Immunol, 2012-10-21;13(12):1213-21.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  58. CCR2+CCR5+ T Cells Produce Matrix Metalloproteinase-9 and Osteopontin in the Pathogenesis of Multiple Sclerosis.
    Authors: Sato W, Tomita A, Ichikawa D, Lin Y, Kishida H, Miyake S, Ogawa M, Okamoto T, Murata M, Kuroiwa Y, Aranami T, Yamamura T
    J Immunol, 2012-10-15;189(10):5057-65.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  59. Biomarkers on melanoma patient T cells associated with ipilimumab treatment.
    Authors: Wang W, Yu D, Sarnaik A, Yu B, Hall M, Morelli D, Zhang Y, Zhao X, Weber J
    J Transl Med, 2012-07-12;10(0):146.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  60. Targeting of macrophage galactose-type C-type lectin (MGL) induces DC signaling and activation.
    Authors: Napoletano C, Zizzari IG, Rughetti A, Rahimi H, Irimura T, Clausen H, Wandall HH, Belleudi F, Bellati F, Pierelli L, Frati L, Nuti M
    Eur. J. Immunol., 2012-04-01;42(4):936-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  61. Identification of human NK17/NK1 cells.
    Authors: Pandya AD, Al-Jaderi Z, Hoglund RA, Holmoy T, Harbo HF, Norgauer J, Maghazachi AA
    PLoS ONE, 2011-10-21;6(10):e26780.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  62. Primary B-cell deficiencies reveal a link between human IL-17-producing CD4 T-cell homeostasis and B-cell differentiation.
    Authors: Barbosa RR, Silva SP, Silva SL, Melo AC, Pedro E, Barbosa MP, Pereira-Santos MC, Victorino RM, Sousa AE
    PLoS ONE, 2011-08-03;6(8):e22848.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  63. Hypoxia modulates the gene expression profile of immunoregulatory receptors in human mature dendritic cells: identification of TREM-1 as a novel hypoxic marker in vitro and in vivo.
    Authors: Bosco MC, Pierobon D, Blengio F, Raggi F, Vanni C, Gattorno M, Eva A, Novelli F, Cappello P, Giovarelli M, Varesio L
    Blood, 2010-12-10;117(9):2625-39.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  64. CCR2 identifies a stable population of human effector memory CD4+ T cells equipped for rapid recall response.
    Authors: Zhang HH, Song K, Rabin RL, Hill BJ, Perfetto SP, Roederer M, Douek DC, Siegel RM, Farber JM
    J. Immunol., 2010-10-27;185(11):6646-63.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  65. Loss of CD4+ T cell IL-6R expression during inflammation underlines a role for IL-6 trans signaling in the local maintenance of Th17 cells.
    Authors: Jones GW, McLoughlin RM, Hammond VJ, Parker CR, Williams JD, Malhotra R, Scheller J, Williams AS, Rose-John S, Topley N, Jones SA
    J. Immunol., 2010-01-18;184(4):2130-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  66. Expression of soluble vascular endothelial growth factor receptor-1 in human monocyte-derived mature dendritic cells contributes to their antiangiogenic property.
    Authors: Kishuku M, Nishioka Y, Abe S, Kishi J, Ogino H, Aono Y, Azuma M, Kinoshita K, Batmunkh R, Rentsenhand B, Makino H, Ranjan P, Minakuchi K, Sone S
    J. Immunol., 2009-12-15;183(12):8176-85.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  67. CCL21 overexpressed on lymphatic vessels drives thymic hyperplasia in myasthenia.
    Authors: Berrih-Aknin S, Ruhlmann N, Bismuth J, Cizeron-Clairac G, Zelman E, Shachar I, Dartevelle P, de Rosbo NK, Le Panse R
    Ann. Neurol., 2009-10-01;66(4):521-31.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  68. Changes in chemokines and chemokine receptor expression on tonsillar B cells upon Epstein-Barr virus infection.
    Authors: Ehlin-Henriksson B, Liang W, Cagigi A, Mowafi F, Klein G, Nilsson A
    Immunology, 2009-08-01;127(4):549-57.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  69. Phenotypic and functional markers for 1alpha,25-dihydroxyvitamin D(3)-modified regulatory dendritic cells.
    Authors: Pedersen AW, Holmstrom K, Jensen SS, Fuchs D, Rasmussen S, Kvistborg P, Claesson MH, Zocca MB
    Clin. Exp. Immunol., 2009-07-01;157(1):48-59.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  70. An unusual CD56(bright) CD16(low) NK cell subset dominates the early posttransplant period following HLA-matched hematopoietic stem cell transplantation.
    Authors: Dulphy N, Haas P, Busson M, Belhadj S, Peffault de Latour R, Robin M, Carmagnat M, Loiseau P, Tamouza R, Scieux C, Rabian C, Di Santo JP, Charron D, Janin A, Socie G, Toubert A
    J. Immunol., 2008-08-01;181(3):2227-37.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  71. Intravenous immunoglobulins suppress T-cell priming by modulating the bidirectional interaction between dendritic cells and natural killer cells.
    Authors: Tha-In T, Metselaar HJ, Tilanus HW, Groothuismink ZM, Kuipers EJ, De Man RA, Kwekkeboom J
    Blood, 2007-08-02;110(9):3253-62.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  72. Alpha1beta1 integrin is crucial for accumulation of epidermal T cells and the development of psoriasis.
    Authors: Conrad C, Boyman O, Tonel G, Tun-Kyi A, Laggner U, de Fougerolles A, Kotelianski V, Gardner H, Nestle FO
    Nat. Med., 2007-07-01;13(7):836-42.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  73. Potential role for IL-7 in Fas-mediated T cell apoptosis during HIV infection.
    Authors: Fluur C, De Milito A, Fry TJ, Vivar N, Eidsmo L, Atlas A, Federici C, Matarrese P, Logozzi M, Rajnavolgyi E, Mackall CL, Fais S, Chiodi F, Rethi B
    J. Immunol., 2007-04-15;178(8):5340-50.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  74. Aberration of CCR7 CD8 memory T cells from patients with systemic lupus erythematosus: an inducer of T helper type 2 bias of CD4 T cells.
    Authors: Sen Y, Chunsong H, Baojun H, Linjie Z, Qun L, San J, Qiuping Z, Junyan L, Zhang X, Jinquan T
    Immunology, 2004-06-01;112(2):274-89.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  75. Glycolysis controls the induction of human regulatory T cells by modulating the expression of FOXP3 exon 2 splicing variants
    Authors: Veronica De Rosa, Mario Galgani, Antonio Porcellini, Alessandra Colamatteo, Marianna Santopaolo, Candida Zuchegna et al.
    Nature Immunology
  76. A bioengineered probiotic for the oral delivery of a peptide Kv1.3 channel blocker to treat rheumatoid arthritis
    Authors: Yuqing Wang, Duolong Zhu, Laura C. Ortiz-Velez, Jacob L. Perry, Michael W. Pennington, Joseph M. Hyser et al.
    Proceedings of the National Academy of Sciences
  77. Targeting of Aberrant alpha v beta 6 Integrin Expression in Solid Tumors Using Chimeric Antigen Receptor-Engineered T Cells
    Authors: Lynsey M. Whilding, Ana C. Parente-Pereira, Tomasz Zabinski, David M. Davies, Roseanna M.G. Petrovic, Y. Vincent Kao et al.
    Molecular Therapy

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Human CCR7 Fluorescein-conjugated Antibody
By samuel chiang on 07/14/2016
Application: Flow Sample Tested: Peripheral blood mononuclear cells (PBMCs) Species: Human

Cryopreserved PBMC were thawed and aliquoted to 0.5 million cells per well. Cells were stained with anti-CD3 APC and differing amounts of CCR7-FITC in each well (amount used as seen in figure). Cells were fixed in BD fix, and acquired on a BDFortessa.

Flow Cytometry Human CCR7 Fluorescein-conjugated Antibody FAB197F