Human CCR7 PerCP-conjugated Antibody

Name: Human CCR7 PerCP-conjugated Antibody
Brand: R&D Systems
SKU: FAB197C
Rating: 5 (1 reviews)

Catalog #: FAB197C
1 Citation 1 Review Datasheet / COA / SDS

Clone 150503 was used by HLDA to establish CD designation

Discontinued Product

FAB197C has been discontinued.

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Detection of CCR7 in Human Blood Lymphocytes by Flow Cytometry.

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Product Details

Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CCR7 PerCP-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CCR7.

Source

Monoclonal Mouse IgG_2A Clone # 150503

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human CCR7 transfectants
Met1-Pro378
Accession # AAA58615

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

PerCP (Peridinin-chlorophyll Protein Complex) (Excitation= 482 and 564 nm, Emission= 675 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CCR7 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD4 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB3791F) and either (A) Mouse Anti-Human CCR7 PerCP-conjugated Monoclonal Antibody (Catalog # FAB197C) or (B) Mouse IgG_2APerCP Isotype Control (Catalog # IC003C). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

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Detection of CCR7 in HEK293 Human Cell Line Transfected with Human CCR7 and eGFP by Flow Cytometry. The specificity of Mouse Anti-Human CCR7 Clone 150503 was demonstrated by its ability to react with (A) HEK293 human embryonic kidney cell line transfected with human CCR7 and not react with (B) irrelevant transfectants (HEK293 human embryonic kidney cell line transfected with human CCR7 and eGFP). Antibody binding was monitored using Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR7

CCR7 is a 7 transmembrane G protein coupled chemokine receptor. CCR7 is expressed on T cells and mature dendritic cells and transduces chemotactic signals in response to CCL19 and CCL21. Human CCR7 shares 87% amino acid sequence identity with mouse CCR7.

Entrez Gene IDs

1236 (Human); 12775 (Mouse); 287673 (Rat)

Alternate Names

BLR2; BLR2C-C chemokine receptor type 7; C-C CKR-7; CC-CKR-7; CCR7; CCR-7; CD197 antigen; CD197; CDw197; CDw197CC chemokine receptor 7; chemokine (C-C motif) receptor 7; CMKBR7; CMKBR7chemokine (C-C) receptor 7; EBI1; EBI1EBV-induced G protein-coupled receptor 1; EBV-induced G-protein coupled receptor 1; Epstein-Barr virus induced gene 1; Epstein-Barr virus induced G-protein coupled receptor; Epstein-Barr virus-induced G-protein coupled receptor 1; EVI1; lymphocyte-specific G protein-coupled peptide receptor; MIP-3 beta receptor

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human CCR7 PerCP-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Monocyte Differentiation towards Protumor Activity Does Not Correlate with M1 or M2 Phenotypes
Authors: G Karina Chimal-Ram
J Immunol Res, 2016-06-08;2016(0):6031486.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry