Human CCR9 PerCP-conjugated Antibody

Name: Human CCR9 PerCP-conjugated Antibody
Brand: R&D Systems
SKU: FAB179C
Price: 146 USD

Catalog #: FAB179C Datasheet / COA / SDS

Clone 112509 was used by HLDA to establish CD designation

Discontinued Product

FAB179C has been discontinued.

View all CCR9 products.

Detection of CCR9 in MOLT‑4 Human Cell Line by Flow Cytometry.

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Human CCR9 PerCP-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Specifically stains human CCR9 transfectants and MOLT-4 cells. It does not react with the parental BaF3 cells or human CCR7-transfected BaF3 cells.

Source

Monoclonal Mouse IgG_2A Clone # 112509

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

BaF3 mouse pro-B cell line transfected with human CCR9
Met1-Leu369
Accession # P51686

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

PerCP (Peridinin-chlorophyll Protein Complex) (Excitation= 482 and 564 nm, Emission= 675 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CCR9 in MOLT‑4 Human Cell Line by Flow Cytometry. MOLT-4 human acute lymphoblastic leukemia cell line was stained with Mouse Anti-Human CCR9 PerCP-conjugated Monoclonal Antibody (Catalog # FAB179C, filled histogram) or isotype control antibody (Catalog # IC003C, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR9

CCR9 is a G protein-linked seven transmembrane domain cytokine receptor. CCR9 serves as a receptor for CCL25/TECK. It is expressed on mature and immature thymocytes and some peripheral T and B cells.

Entrez Gene IDs

10803 (Human); 12769 (Mouse); 282832 (Rat)

Alternate Names

C-C chemokine receptor type 9; C-C CKR-9; CC-CKR-9; CCR9; CCR-9; CD199; CDw199 antigen; CDw199; chemokine (C-C motif) receptor 9; G protein-coupled receptor 28; GPR28; GPR-9-6G-protein coupled receptor 28