Human CD109 Antibody

Name: Human CD109 Antibody
Brand: R&D Systems
SKU: AF4385
Rating: 4 (1 reviews)

Catalog #: AF4385
3 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4385
AF4385-SP

Detection of Human CD109 by Western Blot.

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All CD109 Antibodies

Product Details

Citations (3)

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Supplemental Products

Reviews (1)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD109 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD109 in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD109
Val22-Ser1268 (Tyr703Ser, Thr1241Met)
Accession # Q6YHK3

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

U‑87 MG human glioblastoma/astrocytoma cell line

Simple Western

20 µg/mL

U‑87 MG human glioblastoma/astrocytoma cell line

Flow Cytometry

2.5 µg/10⁶ cells

See below

Immunohistochemistry

5-15 µg/mL

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human CD109 by Western Blot. Western blot shows lysates of U‑87 MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human CD109 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4385) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for CD109 at approximately 161 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Flow Cytometry

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Detection of CD109 in A431 Human Cell Line by Flow Cytometry. A431 human epithelial carcinoma cell line was stained with Human CD109 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF4385, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010).

Immunohistochemistry

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CD109 in Human Squamous Cell Carcinoma. CD109 was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using 15 µg/mL Sheep Anti-Human CD109 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4385) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western

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Detection of Human CD109 by Simple Western^TM. Simple Western lane view shows lysates of U‑87 MG human glioblastoma/astrocytoma cell line, loaded at 0.2 mg/mL. A specific band was detected for CD109 at approximately 161 kDa (as indicated) using 20 µg/mL of Sheep Anti-Human CD109 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4385). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD109

CD109 is a GPI-anchored member of the alpha-2-macroglobulin (A2M) and complement family of proteins (1). Mature human CD109 contains a bait region with recognition sequences for multiple proteases, an internal thioester bond, and a domain similar to the receptor binding domain of A2M (2). Cleavage of A2M family proteins within the bait region activates the thioester bond to promote covalent bonding to nucleophilic groups in adjacent molecules (3, 4). Within the region included in this recombinant protein, human CD109 shares 71-73% amino acid (aa) sequence identity with mouse and rat CD109. It shares 27-33% aa sequence identity with A2M and complement factors C3, C4, and C5. Alternate splicing of human CD109 generates two isoforms with short deletions and one that is truncated within the bait region. CD109 is expressed on activated T cells and platelets, hematopoietic stem cells, megakaryocyte precursors, vascular endothelial cells, basal and myoepithelial cells of secretory glands, and squamous cell carcinomas (2, 5-9). It is produced as a 170-180 kDa glycoprotein that is autocatalytically processed to 150 kDa and 120 kDa forms (2, 6, 10). CD109 on keratinocytes binds TGF-beta and associates with TGF-beta RI and TGF-beta RII, resulting in inhibition of TGF-beta signaling (11). Polymorphisms of CD109 include the platelet-specific Gov antigen and the blood group ABH antigens (12, 13). Alloantibodies directed against these antigens result in unsuccessful platelet transfusions, neonatal alloimmune thrombocytopenia, and posttransfusion purpura (14).

References

Travis, J. and G.S. Salvesen (1983) Annu. Rev. Biochem. 52:655.
Lin, M. et al. (2002) Blood 99:1683.
Christensen, U. and L. Sottrup-Jensen (1984) Biochemistry 23:6619.
Wallis, R. et al. (2007) J. Biol. Chem. 282:7844.
Murray, L.J. et al. (1999) Exp. Hematol. 27:1282.
Haregewoin, A. et al. (1994) Cell. Immunol. 156:357.
Hasegawa, M. et al. (2007) Pathol. Int. 57:245.
Brashem-Stein, C. et al. (1988) J. Immunol. 140:2330.
Hashimoto, M. et al. (2004) Oncogene 23:3716.
Solomon, K.R. et al. (2004) Gene 327:171.
Finnson, K.W. et al. (2006) FASEB J. 20:1525.
Schuh, A.C. et al. (2002) Blood 99:1692.
Kelton, J.G. et al. (1998) J. Lab. Clin. Med. 132:142.
Rozman, P. (2002) Transpl. Immunol. 10:165.

Entrez Gene IDs

135228 (Human); 235505 (Mouse); 363104 (Rat)

Alternate Names

150 kDa TGF-beta-1-binding protein; activated T-cell marker CD109; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 7; CD109 antigen (Gov platelet alloantigens); CD109 antigen; CD109 molecule; CD109; CPAMD7; CPAMD7r150; DKFZp762L1111; FLJ38569; FLJ41966; Gov platelet alloantigens; p180; Platelet-specific Gov antigen; RP11-525G3.1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human CD109 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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An optimized isolation of biotinylated cell surface proteins reveals novel players in cancer metastasis.
Authors: Karhemo PR, Ravela S, Laakso M et al.
J Proteomics.
CD109-GP130 interaction drives glioblastoma stem cell plasticity and chemoresistance through STAT3 activity
Authors: P Filppu, JT Ramanathan, KJ Granberg, E Gucciardo, H Haapasalo, K Lehti, M Nykter, V Le Joncour, P Laakkonen
JCI Insight, 2021-05-10;6(9):.
Species: Human
Sample Types: Cell Lysates, Whole Cells, Whole Tissue
Applications: Co-Immunoprecipitation, ICC, IHC, Western Blot
CD109 Overexpression in Pancreatic Cancer Identified by Cell-Surface Glycoprotein Capture
Authors: Randy S Haun, Chun-Yang Fan, Samuel G Mackintosh, Hong Zhao, Alan J Tackett
Journal of Proteomics & Bioinformatics